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Citations for Bio-Rad :
401 - 450 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... The qRT-PCR was performed in a 2-step amplification with 40 cycles of 95 °C for 30 s and 55 °C for 30s using a Bio-Rad IQ thermocycler (Bio-Rad Laboratories Inc. Hercules, CA). Each reaction was loaded on a 384-wells standard qPCR plate and ran in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
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bioRxiv - Immunology 2021Quote: ... Settings for analysis using a C-1000 CFX96 machine (Bio-Rad) were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... overnight at 37 °C in a G1000TM Thermal cycler (BIO-RAD) to digest trehalose to produce free glucose ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then to 97°C for 8 min in a thermocycler (BioRad). We centrifuged the samples and froze the supernatant for further use.
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:500), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Microbiology 2019Quote: ... 72°C,20"]x45 using SSO Advanced SYBR Green Supermix (BioRad), and 300 nM Tox9 and 11 primers listed in Table S1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200 g for 2 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200g for 2 minutes.
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bioRxiv - Physiology 2023Quote: ... and transferred (100V, 1h, 4°C) onto PVDF membranes (Bio-Rad). Blots were blocked in buffer containing 5% BSA (Cat#A9418 ...
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bioRxiv - Biochemistry 2023Quote: ... 59 and 61°C in an S1000 thermal cycler (Bio-Rad). For CETSA with dose-response ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed using EBC buffer (Bio-Rad Cat. # C-1410) supplemented with protease EDTA-free (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... and 72°C 15 s using a PCR machine (T100, Biorad). PCR primer pairs used were 5’-TCCCAGCCTGAAAGCCACAG-3’ and 5’-ATGCTGCTGGGATTCTCTCCC-3’ for Pet1 ...
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bioRxiv - Genetics 2023Quote: ... Membranes were incubated overnight at 4°C with STAR5B Streptavidin:HRP (BioRad) at a 1:15,000 dilution in 1x PBST ...
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bioRxiv - Neuroscience 2024Quote: ... the iScript™C gDNA Clear cDNA Synthesis Kit (BIO-RAD) was employed ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 25 μL of double distilled water (boiled at 95 °C for 5 minutes) containing loading buffer (4X; Bio-Rad Laboratories, Mississauga, ON, Canada) and reducing agent (20X ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Immunology 2022Quote: ... The samples were heated with Laemmli sample buffer with 2-mercaptoethanol at 95°C for 20 min and separated on SDS-PAGE using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA).
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bioRxiv - Neuroscience 2022Quote: Tissue sections stained by mRNA FISH and fixed primary neurons were washed with 1 x PBS and blocked in 1 x PBS supplemented with 5 % (vol/vol) goat serum (Bio-Rad), 1 % (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... 5 µl of protein samples and standards (Protein Standards I and II, Bio-Rad) were added to 25 µl reagent A’ and mixed in the wells of a 96 well plate (Corning Costar) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated along with 5 µl Precision Plus Protein All Blue Prestained Protein Standards (Bio-Rad, 1610373) at 80-120 V in SDS-PAGE running buffer containing 25 mM Tris ...
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bioRxiv - Physiology 2023Quote: Plasma cytokines and chemokines were assessed using a magnetic bead panel (Bio-Rad, Cat. #171K1001M). Plasma samples were diluted 1:4 in assay buffer prior to running the assay according to manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cytokine/chemokine levels were measured in supernatant of lung homogenate by Bioplex Assay (Bio-rad).
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bioRxiv - Microbiology 2023Quote: ... following manufacturer’s instructions measuring levels of 30 cytokines and chemokines using the BioPlex reader (Biorad). An additional 2-fold dilution was performed on the supplied standards to extend its lower detection limit.
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bioRxiv - Plant Biology 2019Quote: ... 16.000 x g) the protein concentration of the clear supernatant was determined by the Bradford protein assay (Bio-Rad). After SDS-PAGE (7.5%) ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates diluted in 4 X Laemmli Sample Buffer (Bio-Rad#161-0747) were loaded onto Bio-Rad 4-20% precast gels ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates diluted in 4 X Laemmli Sample Buffer (Bio-Rad#161–0747) were loaded onto Bio-Rad 4–20% precast gels ...
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bioRxiv - Microbiology 2019Quote: ... 98°C for 10 min) with C1000 Touch thermal cycler (Bio-Rad). Fluorescent signal was detected using QX200 Droplet Reader (Bio-Rad).