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Citations for Bio-Rad :
401 - 450 of 8520 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2019Quote: ... 25 mg ml−1 Bio-Beads SM-2 (Bio-Rad) was added and incubated at 4°C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad) containing 100 mM Dithiothreitol (CAS No ...
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bioRxiv - Microbiology 2022Quote: ... Capacitors were 1-cm Tygon tubing (2 mm ID, BioRad), having Luer connectors on each extremity ...
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bioRxiv - Microbiology 2023Quote: ... and β-tubulin (clone YL1/2, Bio-Rad; 1:5000) antibodies at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Microbiology 2020Quote: ... Data were quantified using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and analyzed with GraphPad Prism 4 software.
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bioRxiv - Molecular Biology 2023Quote: ... The intensity of the protein bands was quantified by Quantity One 1-D Analysis Software (Bio-Rad). Background subtraction was performed for each band ...
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bioRxiv - Molecular Biology 2024Quote: ... The levels of proteins bound to chromatin were quantified using Quantity One 1-D Analysis Software (BioRad) and normalized with their corresponding Histone H2B values ...
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bioRxiv - Molecular Biology 2023Quote: ... and individual band densities were measured and compared using Quantity One 1-D analysis software (Biorad, USA). The resulting values were normalized separately for each set of time points.
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bioRxiv - Cancer Biology 2023Quote: ... Blots were visualized using the VersaDoc Imaging System with Quantity One 1-D Analysis software (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... and the band intensities were quantified and analyzed using Quantity One 1-D software (Bio-Rad Laboratories).
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated 5 min at 95°C and separated on 4-20% Mini-PROTEAN® TGX Stain-Free™ Precast gels (Bio-Rad) or on home-made 7% polyacrylamide gel for OPA1 immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... CST #2118) in 5% bovine serum albumin (total protein antibodies) or 5% dry milk (phospho-specific antibodies) Tris buffered saline (Bio-Rad #1706435) with 0.1% Tween-20 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.5 µL of each forward and reverse primer (10 µM) and 5 µL of SsoADV Universal SYBR® Green Supermix (Bio-Rad) were used ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed again with TBST buffer for 5 times X 5 min and developed using Clarity Western ECL substrate (Bio-Rad, 1004384863). Images were captured on a Luminescent Image Analyzer (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’)/ 806R (5’-GGACTACHVGGGTWTCTAAT-3’) by using a T100 Thermal Cycler PCR thermocycler (BIO-RAD, USA). The PCR reaction mixture (20 µL ...
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bioRxiv - Cancer Biology 2023Quote: ... for 7 minutes and separated with 4-20% Mini-PROTEAN TGX protein Gel (Bio-Rad, Cat # 5671094), transferred to nitrocellulose membranes according to standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: The WB samples were loaded on a 4-20% or 7% Midi CriterionTM TGXTM Precast gel (BioRad) and ran at 180V for 45min in 1x running buffer (190mM glycine ...
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bioRxiv - Neuroscience 2021Quote: ... with primers listed in Table 4-1 on a CFX96 system (BioRad). Thermocycler conditions were 95°C for 3 min followed by 40 cycles of 95°C for 10 sec and 55°C for 30 sec ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to a 1:4 mixture of Laemmli Sample Buffer (BioRad) and 2- Mercaptoethanol (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% non-fat milk (BioRad) in 1X PBS with 0.1% of Tween 20 (PBS-T ...
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bioRxiv - Molecular Biology 2019Quote: ... gel purified on a 5% polyacrylamide TBE gel (BioRad) and sequenced on an Illumina HiSeq (Edinburgh Genomics) ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 µl of TEMED (cat#: 1610800, Bio-Rad). The stacking gel (2.5 ml ...
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bioRxiv - Bioengineering 2019Quote: ... 5 μl SYBR Green Supermix (Bio-Rad; 170-8886), and an additional 1.75 μL ddH2O to 3 μL cDNA (30× or 100× diluted) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 50μL of a 5% chelex solution (Bio-Rad laboratories), and 5μL of proteinase K (20 mg ml-1 ...
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bioRxiv - Immunology 2021Quote: ... containing 5% Blotting-Grade Blocker (Bio-Rad, # 170-6404) for 1□h ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 5 µL 2X SSOAdvanced SYBR Green PCR mix (BioRad), and 10 µM each of primer ( total volume ...
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bioRxiv - Cancer Biology 2021Quote: ... After blocking in 5% non-fat milk (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... 5% nonfat milk (Nestle) in TBST buffer (Bio-rad) was used to block the membrane for 1 hour in room temperature ...