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Citations for Bio-Rad :
401 - 450 of 4148 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in 5% milk (Bio-Rad, Hercules, CA, USA) in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then blocked with 5% NFDM (Bio-Rad Laboratories, 1706404) at rt for 1 h and blotted with the following primary antibodies at 4°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... using the Precision Plus Dual Color protein standard (BioRad, 5 µl/lane), run for 50 min at 120V ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked in 5% non-fat dry milk (Biorad; cat. #1706404) diluted in 1x TBS-T (137 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked with 5% (v/v) blotting-grade blocker (Bio-Rad) in TBS (10 mM Tris-HCl and 150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with 5% w/v non-fat milk (Bio-Rad) in Tris-buffered saline (TBS ...
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bioRxiv - Genomics 2024Quote: ... with CHEF DNA 8-48 kb and CHEF DNA 5 kb (BioRad) size standards ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% non-fat milk (Bio-Rad,1706404) in TBS containing 0.1% Tween20 (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% nonfat dry milk (NFDM, BIO-RAD) TBS with Tween-20 (TBST ...
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bioRxiv - Immunology 2023Quote: ... The membrane was blocked for 5 minutes with EveryBlot Blocking Buffer (BioRad) or 5% BSA in TBS-T ...
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bioRxiv - Genomics 2024Quote: ... Each reaction contains 5 μl of iTaq universal Sybr green supermix (Biorad), 1.25 μl of primers (500 nM) ...
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bioRxiv - Microbiology 2020Quote: ... 0.25 U Taq DNA polymerase and 1 µL of DNA template (5-10 ng/µL) on a T100 TM Thermal Cycler (Bio-Rad). Thermal cycling conditions for bacteria and archaea were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Molecular Biology 2022Quote: ... at a 1:5 w/w ratio for 2 hours before addition of 100 mg mL-1 Bio-Beads SM-2 resin (Bio-Rad) and then incubated overnight at 4 °C with gentle agitation to remove detergent ...
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bioRxiv - Bioengineering 2022Quote: ... All the eluted fractions were tested using a Bradford reagent assay (diluted 1:5 with ddH2O) (190 μL Bradford, 10 μL sample) (Bio-rad). Positive fractions were desalted using 100 kDa amicons (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of SsoAdvanced Universal SYBR Green Supermix and 1 μl of the following DNA templates (Prime PCR Assay, Bio-Rad): mt-Co2 (mitochondrial ...
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bioRxiv - Neuroscience 2021Quote: ... ChAT) for 5 minutes or ECL Standard (β -actin) for 1 minute and then imaged using the ChemiDocMP Imaging System (BioRad). One sample from the Vehicle group was excluded from analysis for the BDNF western blot due to a transfer bubble over the band of interest for that sample ...
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bioRxiv - Genetics 2022Quote: ... Blots with HRP-linked secondary antibodies were additionally incubated for 1-5 minutes with Clarity or Clarity Max ECL Western Blotting substrates (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Genetics 2020Quote: ... membranes were washed in × 1 Tris-buffered saline-Tween (TSBT) and then blocked for ~10 min in 5% blocking buffer (Bio-Rad) in 1 × TBST ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA samples were diluted 1:10 and 5 μL was used as template with ddPCR Supermix for Probes (No dUTP) (Bio-Rad) and TaqMan Gene Expression Assays (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
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bioRxiv - Microbiology 2020Quote: Two hundred µL of overnight bacterial cultures were transferred 5 mL of a 1:10 dilution of TSB medium (Bio-Rad, USA) supplemented with 1 mg mL-1 L-tryptophan and incubated at 30°C overnight (Gordon & Weber 1951) ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... 5%β-mercaptoethanol) and migrated on 4-15% Tris-glycine gradient gels (BioRad).
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bioRxiv - Molecular Biology 2021Quote: ... and blocked in PBS/0.1%Tween20/5% Blotting-Grade Blocker (BioRad; Cat #: 1706404) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404, BioRad) powder in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713) and solidifying 15-30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713) and solidifying 15-30 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 5 µL of iTaqTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, California) and a primer pair with a concentration of 0.22 µM each ...
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bioRxiv - Developmental Biology 2019Quote: ... The blot was blocked with 5% Blotting Grade Non Fat Dry Milk (BioRad) and developed with 1:20,000 anti-PDI (Sigma P7496 ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μL of cDNA was mixed with SsoAdvanced SYBR Green Supermix (Bio-Rad) and indicated primers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked for 5 minutes using undiluted EveryBlot Blocking Buffer (Bio-Rad) and then incubated with primary antibodies (specified below ...
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bioRxiv - Physiology 2021Quote: ... then 85°C for 5 min on a C1000 thermal cycler (Bio-Rad), then stored at -20°C.