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Citations for Bio-Rad :
401 - 450 of 744 citations for 6 cyclopropylpyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... washed 3 × 10 min with TBST and exposed with a ECL kit (Bio-rad, cat # 1705061) on X-ray films (Kodak ...
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bioRxiv - Microbiology 2024Quote: ... 10% glycerol) then boiled with 1/3 volume of 4x Laemmli sample buffer (Bio-Rad, 1610747) for 10 mins ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resuspended in a small amount of buffer (6 M urea, 50 mM Tris–Cl pH 7.5) and quantified by the BCA quantification method (Bio-Rad, Hercules, CA). Urea concentration was reduced to 4 M and the pH was adjusted to 8.8 with 25 mM Tris–HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting on the basis of the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration of the resultant solution containing labelled αSyn was adjusted to 2 mg/mL and excess unbound dye was removed using Bio-Spin 6 size exclusion spin columns (Bio-Rad Laboratories). ATT565-αSyn was added to LUHMES cells on day six in vitro at a final concentration of 2 µM ...
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
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bioRxiv - Immunology 2021Quote: ... Quantification of band intensities in 3 Western blot replicates was performed using Image Lab software (Bio-Rad). PALS-5 band intensities for each sample were normalized to the ratio of the tubulin expression levels between N2 DMSO control and a given sample.
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Microbiology 2019Quote: ... A 3 μl volume of this master mix was loaded into a 384 well plate (BioRad HSP3805) using a multichannel pipette according to the layouts in Figure 2 ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-gels (3% stacking gel, 12% separation gel) were run in a Mini PROTEAN® System (BioRad) at 100 V for 10 min and 200 V till end of the separation ...
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bioRxiv - Systems Biology 2020Quote: Plates were incubated for 2-3 days at 30 °C and imaged using a ChemiDoc MP (BioRad). Individual colonies from the plates generated to enable high second stress survival quantitation were counted using in-house developed MATLAB scripts ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times and imaged by chemiluminescence (Pierce) by using a Chemidoc imaging system (BioRad). The images were further analyzed for band intensities using ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates from 3×106 cells were separated on 4-20 % gradient Tris-glycine polyacrylamide gels (BioRad 4568094), electroblotted onto a PVDF membrane (Pierce 88518) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 M NaCl) for 30 minutes and the DNA was transferred to a Zetaprobe membrane (Bio-Rad) in 20X SSC ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified product was run on 3% certified™ low range ultra agarose (Bio-Rad Laboratories Ltd) in TBE buffer and a size-selection was performed to ensure that the cDNA used for sequencing primarily contains miRNAs rather than other RNA contaminants ...
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bioRxiv - Immunology 2020Quote: ... and detergent removed by adsorption to polystyrene BioBeads SM-2 (3 mg; Bio-Rad; overnight, 4°C). Proteoliposomes were collected by ultracentrifugation (180,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... the blot was rinsed 3 times in TBS-T and incubated with StrepTactin-HRP conjugate (Bio-Rad) and HRP-conjugated secondary antibody for 1 hour at 22°C ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Immunology 2022Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Molecular Biology 2022Quote: ... and added to 7 cm ReadyStrip IPG Strips with a linear pH range of 3-10 (BioRad) for passive rehydration overnight at 20 °C in a Protean IEF Cell equipment (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Isoelectric focusing was performed in immobilized pH gradient strips (17 cm, pH 3–10 NL; Bio-Rad). The focused strips were stored at −80°C until second-dimension electrophoresis was performed ...
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bioRxiv - Plant Biology 2023Quote: ... Leaves from 3 dai (days after infiltration) were used for GFP detection by ChecmiDoc MP machine (BioRad), and ImageJ Fiji was used for quantification of GFP intensity ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (Bio-Rad) after activation with ECL substrate solution.
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bioRxiv - Molecular Biology 2023Quote: ... ∼3 million cells were harvested and proteins were extracted by boiling in 2x Laemmli sample buffer (BioRad). Protein levels were measured by Western blotting with different antibodies (Rabbit anti- BRWD3 ...
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bioRxiv - Systems Biology 2023Quote: ... Time points were measured every 2-3 days by flow cytometry analysis of >10,000 cells (BioRad ZE5). For experiment with BRM014 (MedChemExpress #HY-119374) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were resolved in 2.5-3% agarose gel and imaged using Gel Doc EZ (Bio-Rad). Exposure time was adjusted to ensure band intensities were not saturated ...
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bioRxiv - Neuroscience 2023Quote: ... culture were then rinsed three times with PBS and treated with 3% (w/v) hydrogen peroxide (BioRad) in PBS for 5 min to minimize endogenous peroxidase activity ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Systems Biology 2024Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...