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Citations for Bio-Rad :
401 - 450 of 9152 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from total RNA (1-2 μg) with iScript Advanced cDNA Synthesis Kit (Bio-Rad; 1725038). Quantitative RT-PCR (qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... detergent was removed by addition of 0.8 grams ml-1 reaction buffer of Bio-Beads SM-2 (Biorad) prewashed in storage buffer and incubation on a roller for 2 hours at room temperature (RT) ...
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bioRxiv - Biophysics 2024Quote: ... The hC1q sample was mixed at a 1:2 ratio with 2x Laemeli Sample Buffer from Bio-Rad. For the reducing conditions the Laemeli sample buffer also contained 2-β-mercaptoethanol ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ // Reverse: 5’-CGA AGG TGT GAC TTC CAT G-3’) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated (4°C, overnight) with 5 % milk powder (BioRad) in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... 5-20μg protein were loaded onto 4-20% SDS-PAGE gels (Biorad) and run at 100V ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin was quenched with 12.5 µL formic acid (ULC-MS grade) and the beads were removed by filtration using a Bio-Spin column (BioRad, 400 g, 5 min), collecting the flow-through in a new 2 mL tube ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: ... each lysate was diluted at a 1:1 ratio with a solution of 2× Laemmli sample buffer (#1610737EDU, Bio-Rad, Hercules, CA, USA), containing 5% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 8% of 2% w/v bis-acrylamide (Bio-Rad, #1610142), 0.5% of 10% ammonium persulfate (APS ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were boiled with 2× Laemmli sample buffer (Bio-Rad) containing 2.5% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... for 2 min and detected by ChemiDoc imaging system (Biorad). For equal loading analysis ...
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bioRxiv - Biophysics 2020Quote: ... and 2% bis-acrylamide (#161-0142, Bio-Rad, Munich, Germany) in PBS that are stored at 4°C for a maximum time of 2 months ...
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bioRxiv - Cancer Biology 2021Quote: ... using 2× iQ Custom Sybr Green Supermix (Bio-Rad Laboratories). Values were normalized on mRNA expression of human β-actin and HPRT (reference genes) ...
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bioRxiv - Biochemistry 2021Quote: ... 200 mg Bio-Beads™ SM-2 Resin (Bio-Rad) was added and incubated at 4°C overnight while shaking at 650 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... 2 x iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) was used for the Reverse Transcription-Polymerase Chain Reaction (RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μl 2 x EVA-Green master mix (Bio-Rad), 1 μl HindIII-HF (NEB) ...
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bioRxiv - Genomics 2022Quote: ... The lysate was added to 2 chromatography columns (7321010, BioRad) packed with 25 mL each of chitin resin (S6651S ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were diluted with 2 × Laemmli sample buffer (Bio-Rad) and β-mercaptoethanol (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were diluted with 2□×□Laemmli sample buffer (Bio-Rad) and β-mercaptoethanol (Sigma) ...
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bioRxiv - Systems Biology 2024Quote: ... buffer + 2% (v/v) Sodium dodecyl sulfate (SDS, Bio-Rad). Proteins were denatured at 95 °C and 600 rpm for 10 min before adding a final concentration of 2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Detergent was removed using adsorbing SM-2 Biobeads (Bio-Rad): the protein-lipid mix was incubated twice with fresh Biobeads at 10:1 (w:w ...
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bioRxiv - Molecular Biology 2022Quote: ... goat anti-human IgG F(ab’)2 (STAR126, Bio-Rad), anti-M13-pVIII-HRP (Cytiva 27-9421-01 ...
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bioRxiv - Neuroscience 2023Quote: ... including 7.5 μl of 2× iQSYBR Green Mix (Bio-Rad) and 100–300 nM forward and reverse primers ...
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bioRxiv - Neuroscience 2023Quote: ... 60 mg of Bio-Beads SM-2 (#1523920, Bio-Rad) was added ...
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bioRxiv - Immunology 2024Quote: ... 4X Laemmli Sample Buffer containing 10% 2-Mercaptoethanol (Bio-Rad) was added to each sample to a final concentration of 1X ...
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bioRxiv - Immunology 2024Quote: ... We placed 2 g/L Chelex 100 resin (Bio-Rad) in the dialysis buffer to chelate divalent metal ions ...
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bioRxiv - Biophysics 2024Quote: ... then detergent was removed with BioBeads SM-2 (Bio-Rad) at 80 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... and 2 % bis-acrylamide solutions (594 µL, #1610142, Bio-rad) were formulated in distilled water (2736 µL ...
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bioRxiv - Bioengineering 2024Quote: ... and 30 % v/v bis-acrylamide 2 % (Bio-rad: 1610142) were added to produce the gels and mixed with 2 % v/v fluorescent carboxylated 200 nm beads (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and 8 % v/v bis-acrylamide 2 % (Bio-rad: 1610142) were added to produce the gels and mixed with 2 % v/v fluorescent carboxylated 200 nm beads (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ - GAT GGA GAA AGC TCT GAG CAT C -3’ Reverse: 5’ - TTG CTC CAC AGA TGG AGT TG -3’ RHAMM PrimePCR primers were obtained from BioRad (Cat#: 10025636)
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bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Cell Biology 2020Quote: ... 5%β-mercaptoethanol) and migrated on 4-15% Tris-glycine gradient gels (BioRad).
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bioRxiv - Neuroscience 2021Quote: ... 5-10µg protein were loaded on SDS-PAGE gels (4-15% acrylamide, Biorad). After 1h of electrophoresis at 150V ...
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Homemade 4% stacking and 6% running gels were run at 60-80V in Tris/Glycine/SDS buffer (BioRad). Transfer was done overnight in 10% methanol in Tris/Glycine buffer (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...