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Citations for Bio-Rad :
4301 - 4350 of 4847 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Polysome profiling was carried out using a Piston Gradient Fractionator (Biocomp) with continuous monitoring at 254 nm using a Model EM-1 Econo UV detector (BioRad). Monosome and polysome fractions of approximately 400 μl were collected with a fraction collector (Gilson FC203B).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody cocktail against CD68 (rat monoclonal; 1:250; catalog no. MCA1957; Bio-rad), GFAP (Chicken polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... wet blotting (at 200 mA per gel for 1 hour) was performed to transfer proteins onto 0.2 µm nitrocellulose membranes (Bio-Rad). Total protein staining was performed according to the manufacturer’s protocol (Revert™ Total Protein Stains kit ...
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bioRxiv - Systems Biology 2023Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). For gene expression assays ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Neuroscience 2023Quote: Four fragments of the tln-1 genomic DNA (gDNA) (pBG-1088) were amplified from N2 genomic DNA using iProof High-Fidelity DNA polymerase (BioRad) or Q5® High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 4.3 µL of cDNA was mixed with 5 µL iTaq Universal SYBR Green Supermix (BioRad 1725124) and 0.7 µL of 5 µM forward and reverse qPCR primers targeting the gene of interest (333 nM final primer concentration ...
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bioRxiv - Developmental Biology 2023Quote: ... in blocking solution for 1 hour at room temperature, then visualized by addition of ECL substrate (Amersham, GE) and imaged using a ChemiDoc (BioRad). Western blot quantification was performed in ImageLab (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: Shoot apices of reporter lines were dissected and fixed in 4% paraformaldehyde for 1 hour and embedded in 7% ultra low range agarose gel (BioRad). Fifty µm sections were prepared using a vibratome DTK-1000 (Dosaka ...
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary horseradish peroxidase linked antibody was incubated for 1 hour with gentle agitation at room temperature and developed using chemiluminescence blotting substrate (1705061, BioRad or 34095 ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated secondary antibodies used were anti-rabbit and anti-mouse at a dilution of 1:2000 and immunoblots were developed using Chemidoc imaging system (ChemiDoc, BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed 3X in PBST (10 minutes) and incubated for 1 hour with horseradish peroxidase conjugated anti-rabbit secondary antibody (BioRad 170-6515 ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... blots were incubated in 1-shot ECL solution (Kindle Biosciences LLC) and imaged with a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The HRP-coupled secondary antibodies were used at a 1:10,000 dilution followed by development using ECL substrate from Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR was carried out using the PerfeCTa SYBR Green Fastmix (Quantabio, 95071) and gene-specific primers (Table 1) on CFX96 system (Biorad). Relative gene expression was determined using the ΔΔCt method.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were stained with Ponceau S to visualize protein loading and VAChT was revealed with rabbit anti-VAChT (1:1000, SYSY, 139103) by ECL procedure (Clarity Western ECL, Biorad) on a chemiluminescence imager (ChemiDoc ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with secondary antibody diluted 1:7500 in blocking buffer (anti-rabbit, anti-mouse HRP, Bio-Rad). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher 32106) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were electrotransferred onto nitrocellulose membranes using the trans-blot turbo transfer system (constant voltage: 25V, maximum amperage: 1 A; 30 min, Biorad) and membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were used at 1:5000 dilutions and chemiluminescence signal was produced using the Clarity western ECL substrate (BioRad) and detected using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA samples were obtained from 1 µg RNA using the iScriptTM cDNA synthesis kit (Bio-Rad, Cat no. 170-8891) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of proteins at 10 µM mixed in a 3:1 ratio with 4X Laemmli Buffer Sample (Biorad) provided with 10% DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.2–2.2 Mb, S. cerevisiae Ladder: Bio-Rad, 1703605; CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder: Bio-Rad, 1703667) and PFGE was performed using the CHEF Mapper XA System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... we used 1/20th volume of a cDNA reaction to program a PCR reaction using iQ SYBR Green Supermix (Bio-Rad). PCRs were amplified on a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... The product yield for each condition was estimated by resolving 10% of the reaction on a 1% EtBr-agarose gel and quantifying the band intensity of the resulting amplicon with ImageLab software (Bio-Rad). The 1.6-kb amplicon was gel-extracted using the QiaQuick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with 1 μg total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad 170-8841). cDNA equivalent to 20 ng of total RNA was then amplified in duplicate using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 172-5274 ...
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bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
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bioRxiv - Developmental Biology 2021Quote: ... and OPN) and control (HPRT) transcripts were amplified (Supplemental Table 1) using SsoAdvanced Universal SYBR Green Supermix (Biorad, Hercules, CA, USA). Established TaqMan assays were used to determine the expression of Lgr6 and TBP intra-well controls ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad, 1610772).
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Microbiology 2019Quote: ... The copy number of a host gene RPP30 was determined using 1 μL of HEX-labeled PrimePCR RPP30 (Bio-Rad, 10031243) in the reaction to calibrate the amount of DNA input ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Genomics 2020Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using Kapa Illumina Quant kit.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...