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Citations for Bio-Rad :
4201 - 4250 of 4986 citations for Olfactomedin 4 OLFM4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The tertiary antibody solution contained DyLight 550 mouse anti-V5 (1:500, Bio-Rad, #MCA1360D550GA).
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bioRxiv - Biochemistry 2024Quote: ... Non-phospho-specific antibodies were diluted in TBST/ 5% non-fat dry milk powder (BioRad). Phospho-Rab10 levels were determined by the site-specific rabbit monoclonal antibody anti-pRAB10(pT73 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated in anti-rabbit HPR conjugate antibody (1:5000, 170-6515, Bio-Rad) in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies for Westering blotting were goat anti-mouse IgG HRP (Bio-Rad; 1:3000) and polyclonal goat anti-rabbit IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... blots were exposed to goat anti-mouse or goat anti-rabbit secondary antibodies (BIO-RAD) for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit IgG goat antibody (Bio-Rad Laboratories, Hercules, CA, USA) was added to detect bound anti-alpaca IgG rabbit antibody ...
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bioRxiv - Cell Biology 2023Quote: ... and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP) (BioRad, 1:5000). The detection of HRP was accomplished using the PierceTM ECL Western Blotting Substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... detection was performed using horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagent (Bio-Rad). Primary and secondary antibodies used are given in Suppl ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-conjugated secondary antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA).
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bioRxiv - Cell Biology 2023Quote: ... and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP) (BioRad, 1:4000) were used ...
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bioRxiv - Microbiology 2023Quote: ... goat anti-rabbit IgG HRP-conjugated secondary antibody (1: 3,000 dilution, BIO-RAD 170-6515). Proteins were detected with Chemi-Lumi One (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG HRP-conjugated secondary antibody (Bio-Rad) and developed using ECL Western Blotting Substrate (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was immunoprecipitated with anti-CATSPER1 antibody and Protein A magnetic beads (BIO-RAD) with the same method for recombinant protein co-IP ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1 hour at room temperature with HRP-conjugated secondary antibodies (BioRad). Three washing steps of 5 minutes in PBS–Tween20 (0.1–0.3% ...
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bioRxiv - Biochemistry 2023Quote: ... Blots were incubated with corresponding secondary antibodies at concentrations suggested by the manufacturer (Bio-Rad), were developed using the Clarity Western ECL kit (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... followed by overnight staining with pig specific CD45 antibody (clone K252.1E4, Bio-Rad, 1:200). After secondary ab and DAPI applications ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD45-APC (clone UM16-6, LYNX Rapid APC Antibody Conjugation Kit, both Bio-rad) and thrombocyte marker K1-PE (LYNX Rapid RPE Antibody Conjugation Kit ...
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bioRxiv - Microbiology 2023Quote: ... incubated for one hour with horse radish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse: Biorad, 172- 1011 ...
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bioRxiv - Biochemistry 2024Quote: ... secondary antibodies were used and western blots were imaged on a ChemiDoc system (Bio-Rad). Replicate experiments were conducted in parallel and independently.
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bioRxiv - Immunology 2023Quote: ... and then anti-mouse IgG secondary antibody conjugated to HRP (172-1011, Bio-Rad Laboratories), diluted 1:5000 in blocking buffer as per manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... the membrane was incubated with the goat-anti-rabbit secondary antibody (1:10,000, Bio-Rad). After incubation ...
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bioRxiv - Biophysics 2024Quote: ... Alexa Fluor 488-labeled mouse anti-human CD81 antibody (MCA1847A488) was purchased from Bio-Rad. Alexa Fluor 555-labeled mouse anti-human EpCAM antibody (5488S ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad #1706516), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Bio-Rad #1706515) ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:3000 dilution and a secondary goat α-rabbit-HRP antibody (Bio-Rad) at a 1:10000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... and detected after incubation with specific antibodies using ECL Clarity Western blotting Substrate (Bio-Rad). Mouse anti-actin (R5441 ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence include a rat monoclonal anti-Tubulin (BioRad #MCA77G, 1:100 dilution), rabbit polyclonal anti-Senseless (gift from Perry lab ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were utilized: Rat Anti-Myelin Basic Protein (MBP; 1:500, Biorad) and Rabbit Anti-Oligodendrocyte transcription factor 2 (Olig2 ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Sample preparations were run on a 15% separating gel with a 4% stacking gel under reducing conditions using Tris-glycine running buffer (mini-PROTEAN system, Bio-Rad Laboratories, Hercules, CA, USA). Silver staining using sodium thiosulphate sensitisation and silver nitrate was performed according to established methods (22).
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... pH 9.5). Quantification of Western blot images (SFig. 1-4) was carried out using ChemiDoc™ XRS+ Image Lab™ Software (Bio-Rad Laboratories AB, Sweden); statistical analysis was performed with GraphPad Prism software (version 5.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed using the primer pairs listed in Table 4 and iTaq Universal SYBR Green Master Mix on a CFX384™ Real-Time PCR detection system (Bio-Rad Laboratories, Hercules, CA). Fold changes in gene expression were calculated by the ΔΔCt method using the weighted average of Ct values for the housekeeping genes Pol2ra ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Immunology 2023Quote: Plasma from control and infected mice was used at a 1:2 and 1:4 dilution with the Bio-Plex Pro Mouse Cytokine 23 Plex Immunoassay (Bio-Rad Laboratories, Inc., CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...