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Citations for Bio-Rad :
4201 - 4250 of 4293 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2E3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Plant Biology 2024Quote: ... Diluted cDNA (1:50) was used as a template for quantitative PCRs using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Real-time detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of Dicer-Like 2 (DCL2) (Glyma.09g025300) and Argonaute 1 (AGO1) (Glyma.09g167100) was assessed using iTaq Universal SYBR Green Supermix (BioRad, Hercules, CA, USA). To quantify F ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eggs were subjected to experimental electroporation parameters (voltage = 50 V; pulse = 25 ms; number of pulses = 1) using the Gene Pulser Xcell Eukaryotic System (Bio-Rad, cat. 1652661). Immediately ...
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bioRxiv - Cell Biology 2024Quote: ... 60 μl 1x Laemmli buffer (diluted by mixing same volume of 1 x PBS with 2x Laemmli buffer, Bio-Rad, Catalog number: #1610737EDU) was added to the beads and incubated for 10 min at 70°C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg RNA was synthesized to cDNA by reverse-transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA; item # 1708891). Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A temperature gradient of 25 to 70°C (heating rate 1°C per min) was applied using a MJ minicycler (Bio-Rad, Hercules, CA, USA). All experiments were performed triplicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... touchdowns from 56 °C to 50°C for 1 min and 50 cycles with 95 °C for 30 s and 50 °C for 1 min in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Physiology 2019Quote: ... The remaining supernatant was diluted to 1.5 µg × µl−1^ total protein in lysis buffer and 4X Laemmli sample buffer (Bio-Rad Laboratories AB, Oslo Norway) containing 2-Mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: Colonies from TAP agar plates were scraped and suspended in 600 μL liquid TAP media and adjusted to an optical density of 1 at 600 nm using a visible light spectrophotometer (SmartSpec 3000, Bio-Rad, Hercules, CA, USA). The Chlamydomonas suspension was used to inoculate TAP liquid media at a 1:250 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman ...
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bioRxiv - Microbiology 2020Quote: ... was performed to detect viral RNA using the CDC standard N2-based SARS-CoV-2 detection assay [35] that was validated for use with the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, MA, USA) on a CFX96 real-time thermocycler (Bio-Rad, Hercules, CA, USA) using a 20 minute RT step and 45 cycle qPCR in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Biochemistry 2022Quote: ... the membrane visualization was facilitated by 1:1 chemiluminescence luminol enhancer solution and peroxide solution using the Bio-Rad imaging system (Molecular Imager® Chemidoc XRS and Bio-Rad imaging system).
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bioRxiv - Immunology 2022Quote: ... Amplification reactions contained a target specific fraction of cDNA and 1 μM forward and reverse primers in iQ™ SYBR® Green Supermix (Bio-Rad, 1708882). Fluorescence was monitored and analyzed in a CFX connect real-time PCR system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... separated by 8% SDS-PAGE gels at 100V for 1 hour and then transferred to a 0.45 μm pore size nitrocellulose membrane (Bio-Rad, catalog number: 162-0115) at 0.2 A for 1.5 hours in an ice bath ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a 0.2 μM cellulose acetate membrane (Whatman ...
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bioRxiv - Plant Biology 2021Quote: ... was performed with gene-specific primers (Supplementary Table 1) using EF1α as internal reference using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) as previously described in (Zandalinas and Mittler ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad, Richmond, CA, USA). Data were normalized using GAPDH as internal control.
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bioRxiv - Epidemiology 2019Quote: ... All amplified fragments were analysed on 1% agarose gels using Gel Doc EQ quantification analysis software (Bio-Rad, Image Lab™ Software Version 4.1).
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and combined with 50 μM of forward and reverse primers (for primer sequences, please see Supplementary Table 1) as well as iQ™ SYBR® Green Supermix (Bio-Rad Laboratories). Expression values were normalized to the internal control Beta-actin (Actb ...
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bioRxiv - Genetics 2020Quote: Cell viability was measured after collecting conditioned medium from BLRR cells by adding 1/10 volume of alamarBlue reagent (Bio-Rad, Hercules, California, USA) to samples followed by incubation at 37°C with 5% CO2 for 1 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Microbiology 2021Quote: ... The suspensions were then distributed into 0.2 mL tubes at approximately 1 × 108 CFU/ml and incubated in a C1000 Thermal Cycler (Bio-Rad Laboratories, Foster City, CA) at 50
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... Universal Probes Supermix, Foster City, CA, USA), 1 µL primers and probe mixture (Stro18S-1530F, Stro18S-1630R and Stro18S-1586T FAM) (Bio-Rad Laboratories, CA, USA), 4 µL deionised H2O ...
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bioRxiv - Genomics 2020Quote: ... and 250 nM TaqMan probe (Ref-HEX and assay specific-FAM dye labeled) in a 1× Bio-Rad Supermix (Bio-Rad, Hercules, CA, USA). The 20μl PCR reaction was mixed with Bio-Rad droplet generator oil and partitioned into 15,00020,000 droplets using the Bio-Rad QX-100 droplet generator (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... The cellular localization of EqPD-1-EGFP and EqPD-L1-EGFP was then confirmed using the ZOE Fluorescent Cell Imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Genomics 2021Quote: ... was run with a 1% agarose TBE gel over 24 hours (Initial switch 1 sec, final switch 25 sec, 6 volt/cm, 120° included angle, Chef III, Bio-Rad, Hercules, CA, USA) with Midrange PFG and Lambda PFG markers (N0342S ...
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bioRxiv - Microbiology 2022Quote: A duplexed droplet digital PCR was performed to measure the levels of cellular or supernatant HSV-1 genomes on the QX100 droplet digital PCR system (Bio-Rad Laboratories, Hercules, CA) using a primer/probe set specific to HSV-1 gB ...
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... a 1:1000 dilution of α-FLAG M2 monoclonal antibodies and/or 1:10,000 dilution of HRP-labeled α-rabbit secondary antibodies (BioRAD Catalog #170-6515) on nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was then amplified by qPCR using specific primers listed in supplementary Table 1 and the SYBR Green Master Mix (ref 1725274, Bio-Rad, Hercules, California, USA). qPCR was performed using the CFX connect real-time PCR system (Bio-Rad ...