Labshake search
Citations for Bio-Rad :
4101 - 4150 of 4927 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) from extracted RNA was obtained using the iScript™ cDNA Synthesis Kit (Bio-rad, CAT No.:1708890) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... DNAase treatment and cDNA synthesis were done with the iSript gDNA clear cDNA synthesis kit (BIO-RAD, Hercules, CA, USA). Quantitative reverse transcription-polymerase chain reaction (RT-PCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Feldkirchen, Germany). Real-time PCR was performed using Platinum SYBR Green qPCR Super Mix-UDG (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR was performed using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, Cat No.: 172-5150) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were washed three times with TBST and were developed using a chemiluminescent ECL kit (Pierce, Cat # 32106) and images were acquired using a ChemiDocTM imaging system (BioRad).
-
bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... A standardized amount of RNA was transcribed into cDNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad; 170-8843), and cDNA was then amplified using the primers ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
-
bioRxiv - Cancer Biology 2022Quote: ... The blots were treated with an enhanced chemiluminescence kit according to the user manual and developed using a ChemiDoc MP Imaging System (BioRad).
-
bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1ug RNA was in a 20ul reaction volume to generate cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, 1708891). Typically ...
-
bioRxiv - Microbiology 2022Quote: ... we synthesized first-strand cDNA from cellular RNA isolated (100ng) from each sorted sample using iScriptTM cDNA synthesis kit (BioRad). Each rtPCR reaction included 25°C for 5 mins ...
-
bioRxiv - Neuroscience 2022Quote: ... an equal amount (500 ng) of RNA was reverse transcribed to cDNA using iScript reverse-transcription kit (Bio-rad, 1708841). Quantitative real time PCR (45 cycles – LightCycler480 software ...
-
bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
-
bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
-
bioRxiv - Cell Biology 2022Quote: ... following the manufactures instructions and cDNA was synthesized from 1μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad). QPCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
-
bioRxiv - Genomics 2022Quote: ... The blots were developed with an enhanced chemiluminescence kit (Thermo Fischer Scientific) and signals acquired by a ChemiDoc instrument (BioRad). All primary histone variant-specific and H3 marks-specific antibodies were used at 1 µg/ml dilution ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2020Quote: Viral RNA was quantified via a one-step qRT-PCR using the iTaq universal probes one-step kit (Bio-Rad) per the manufacturer’s instructions for a 20µL reaction ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo) and imaged using the ChemiDoc MP Imaging System (BioRad).
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantification of RNA sample quality and concentration was performed using the Experion ‘StdSens’ kit (Bio-Rad Laboratories, Inc., Hercules, CA). Stranded RNA-Seq libraries were created and quantified by qPCR (2×76 bp or 2×151 bp ...
-
bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
-
bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
-
bioRxiv - Biochemistry 2021Quote: ... 500 ng of DNaseI-treated RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative RT-PCR was performed using SsoAdvanced SYBR green supermix (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
-
bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... About 500 ng of total RNA was used for cDNA synthesis (iScript cDNA synthesis kit, Bio-Rad, Hercules, California, USA). cDNA was diluted 1:5 before use in PCR reactions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 500ng for males per sample) was subsequently reverse-transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.)
-
bioRxiv - Molecular Biology 2019Quote: ... The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad, USA). Protein bands were visualized using Gel DocTM XR+ System (Bio-Rad ...
-
bioRxiv - Neuroscience 2019Quote: ... Reverse transcription was performed on 0.5-1μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:10 in nuclease-free water ...
-
bioRxiv - Developmental Biology 2019Quote: ... the samples were run in a 12% SDS-polyacrylamide gel and Western blots were developed using an ECL Western blotting kit (Biorad). Rabbit anti-countin antibodies were used at 1 ...
-
bioRxiv - Genomics 2019Quote: Two hundred clonal adult male worms were used to prepare high molecular weight genomic DNA using CHEF Genomic DNA Plug Kits (BioRad). The DNA was quantified on a FEMTO Pulse® ...
-
bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
-
bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
-
bioRxiv - Physiology 2021Quote: ... the concentration of protein in the whole homogenates was determined with the DC protein assay kit (Bio-Rad, Hercules, CA). Equal amounts of protein from each sample were dissolved in Laemmli buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... Complementary DNA (cDNA) was made by converting at least 100 ng RNA with the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
-
bioRxiv - Cancer Biology 2021Quote: Small pieces of dissected frozen pancreatic tumor tissues were collected and lysed (Bio-Plex Cell Lysis Kit, BioRad, USA; 171304011), followed by a repeated procedure of vortexing ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized on 1μg of isolated RNA by reverse transcription with random hexamer primers using the iScript Select cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the gels were washed in ddH2O and then silver stained following the manufacturers instruction (Silver Stain Plus Kit, 1610449, Biorad).
-
bioRxiv - Microbiology 2021Quote: After the protein concentration of cell lysates and homogenates had been determined using the DC Protein Assay Kit (Bio-Rad) 20 µg of each sample were dissolved in NuPAGE LDS Sample buffer (including 2% β-mercaptoethanol) ...