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Citations for Bio-Rad :
4101 - 4150 of 7745 citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of nuclear extract in SDS loading buffer were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Biochemistry 2024Quote: ... the beads were mixed with 1.5 × loading buffer and then loaded on a 4-15% pre-casted protein gels (BioRad; #456-1084) prior to transfer on PVDF membrane for western blotting using anti-Flag ...
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bioRxiv - Biophysics 2024Quote: SDS-Page was performed with a 4%-12% gradient acrylamide Bis-tris gel with XT MES running buffer (Bio-Rad Laboratories). Both constructs nsp7-11C and nsp7-11N were mixed at 36 µM with 14 µM Mpro and incubated at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... and DNA fragment (amplification products containing homologous DNA fragments) in a 4-mm Gene Pulser and electrotransformed by Gene Pulser Xcell system (Bio-Rad). Transgenic parasites were selected with pyrimidine (1μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of diluted cDNAs were added to each PCR reaction containing the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725271) and primers at a final concentration of 300 nM each ...
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bioRxiv - Neuroscience 2023Quote: ... Ten µg of total proteins quantified by PierceTM BCA protein assay kit (ThermoScientific) were loaded on 4-15% gradient SDS–PAGE gels and transferred to nitrocellulose membranes (Trans-Blot turbo, Bio-Rad). Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved on 4–20% FastPAGE precast gels (Tsingke Biotechnology) and transferred to nitrocellulose membranes in a Trans-Blot Turbo machine (Bio-Rad). Membranes were blocked with 5% nonfat milk dissolved in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of extracts (quantified using Lowry assay) were subjected to SDS-PAGE analysis on 4-15% Criterion TX gels (Bio-Rad), transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... This was accomplished using electroporation (260 mV for 30 ms; 4 mm-gap cuvette) via a Gene Pulser XCell system (Bio-Rad). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µg of each sample was prepared and run by SDS-PAGE on Bio-Rad Criterion 4-15% 26-well gradient gel (Bio-Rad). For some samples (U2OS CT26 ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 μg of protein extracts were run on a 4%–15% polyacrylamide gels (Mini Protein TGX Stain-Free gels, Bio-Rad) and transferred to PVDF (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: Cell lysates from ITFG1-Myc-expressing MDA-MB-231 and non-expressing control MDA-MB-231 cells were separated in a 4-20% SDS-PAGE gel (Bio-Rad). The gels were stained with Imperial Protein Stain (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 5-10μg of total proteins were separated on bis-tris polyacrylamide gradient gels (NuPAGE Novex 4-12% Bis-Tris Gel, Life tech, #NW04120BOX) or TGX stain-free gels (BioRad, #4568126) and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2023Quote: ... 10% ß-mercaptoethanol) was used as the starting material for separation on 4–20 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). The purified virus was loaded into the sample well and was run on the gel placed in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell for 30 min at 200 V ...
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bioRxiv - Microbiology 2023Quote: ... Purified CreS controls and equivalent OD600 nm units of cleared cell lysates by centrifugation were resolved on a 4-20% SDS-polyacrylamide gel (Bio-Rad). In this step ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were blocked with 4% bovine serum albumin (BSA) (Gemini Bio, 700-109P) dissolved in Tris-buffered saline (Bio-Rad #1706435) supplemented with 0.025% Triton X-100 (TBST) ...
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bioRxiv - Biochemistry 2023Quote: ... The total volume of samples was applied to precast gel (Mini-Protean TGX Gels, 4-15% gel, Bio-Rad, CA, USA). SDS-PAGE was carried out following manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the prepared samples were loaded onto the lanes of a 4-15% Mini-PROTEAN Tris PAGE gel (Bio-rad #4561086). Electrophoresis was carried out at 100 V for about 1.5-2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... 30μg of total protein from each sample was placed into Mini-PROTEAN (R symbol) TGX (™) 4-20% gels (Bio-Rad) and subjected to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
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bioRxiv - Microbiology 2023Quote: ... The serial dilutions and supernatants of auxin- and vehicle-treated parasites of a single strain were loaded onto the same precast 4–15% gel (Bio-Rad). Subsequent immunoblotting steps were performed as described in “Immunoblotting” ...
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins ≤ 250 kDa were separated by SDS-PAGE electrophoresis using 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and transferred to Immobilon-P PVDF Membrane (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was quantified by Qubit and 15ug protein was run on a 4-20% Mini-PROTEAN(R) TGX Stain-Free Protein gel (Bio-Rad). Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie Blue.
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie blue dye.
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bioRxiv - Biochemistry 2023Quote: ... the clear supernatant was loaded on a 4–20% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories, Inc., Hercules, USA) including PageRuler™ Plus Prestained Protein Ladder (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg (for FLN-DD experiment) of protein lysates for each treatment condition was fractionated in 4-15% Mini-Protean TGX protein gels (Bio-Rad) and transferred to nitrocellulose membrane using Bio-Rad Trans-Blot Turbo Transfer System with “Turbo Mixed MW” settings (constant 2.5A for 7 minutes) ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Plant Biology 2023Quote: ... and 10 (V/V) % beta-mercaptoethanol for 5 minutes before being loaded into a 4-20% gradient SDS-PAGE gel (Biorad: 4561096) and run according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Mini-Protean TGX gradient gels (4561094, Bio-RAD) and stained with Coomassie Blue ...
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bioRxiv - Plant Biology 2023Quote: ... during 35 minutes at 200 V (Mini-PROTEAN® Tetra Cell, 4-Gel System, Bio-Rad laboratories Inc, Hercules, CA, USA) and were electroblotted onto 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Systems Biology 2023Quote: ... Whole-cell lysates were separated on an SDS-PAGE gradient gel (4–15%) and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). p21 and Cdk2 were detected in 15 μg of whole-cell lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2022Quote: ... pulldown samples collected as described above were loaded on a 4-12% gradient Criterion XT Bis-Tris precast gel (Bio-Rad). The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Biochemistry 2023Quote: 15µg was loaded on a 4-20% acrylamide gradient gel with 18 or 26-wells (Bio-Rad #5671094 & Bio-Rad #5671095) and separated for 20min at 100v followed by 150V for 1 hour ...