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Citations for Bio-Rad :
4051 - 4100 of 5185 citations for Mouse CD252 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Purified RNA (1 μg) was used for reverse transcription using the iScript™ cDNA Synthesis Kit (BIO-RAD). RT-qPCR was performed using SYBR green (ThermoScientific ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were visualized using the Bio-Rad Clarity™ Western ECL kit and the ChemiDoc Imaging System (Biorad).
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bioRxiv - Microbiology 2023Quote: ... All reactions were conducted using the iTaq Universal Probes One-Step RT-qPCR Kit (BioRad, Cat. No. 1725141) on a CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Neuroscience 2023Quote: Total protein concentration for each sample was determined using the DC Protein Assay kit (BioRad, Inc.; Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a LPF PVDF membrane using the Trans-Blot Turbo RTA Transfer kit (Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to ethanol-activated 0.2μm PVDF membranes using the Trans-Blot Turbo RTA Mini Transfer kit (#1704272) from BioRad. Each blot was blocked using 5%/0.1% BSA/Tween in TBS and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quantification of mRNA levels was conducted via an iTaq Universal SYBR Green One-Step Kit (Bio-Rad). The assay was performed on an ABI StepOnePlus PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Immunology 2024Quote: ... We assessed library quantities using digital droplet PCR (ddPCR) with the Library Quantification Kit for Illumina TruSeq (BioRad). We diluted libraries to 2nM and performed paired-end sequencing using a HiSeq 2500 (Illumina).
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted using the OMEGA total RNA kit and reverse transcribed using iScript reverse transcriptase (Bio-Rad). qPCR was performed using TB Green Premix Ex Taq (Takara) ...
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bioRxiv - Genomics 2024Quote: ... chr10:28,469,205-28,662,312 in EquCab3.0) were extracted from 10 ml bacterial cultures with the Quantum Prep Plasmid miniprep kit (BioRad), according to supplier instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Signal detection and quantification was achieved using the Clarity Western ECL substrate kit (Bio-Rad, Catalogue No.: 1705061), a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA was reverse transcribed from 500ng of RNA using iScript IV VILO cDNA synthesis kit (Bio-Rad), with reactions incubated at 25°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... They were loaded in 10% acrylamide gels (TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%, Biorad), with protein ladder (NZYColour Protein Marker II ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcriptase-PCRs (RT-PCR) were carried out using the iScript RT Kit for cDNA synthesis (BioRad, 1708841). Where the final mix include 1000ng of RNA sample and 5µL of iScript RT-PCR mix with reverse transcriptase ...
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bioRxiv - Pathology 2024Quote: ... Total RNA was transcribed reversely to cDNA using iScript™ cDNA Synthesis kits (Cat # 170-8891; Bio-Rad). Quantitative PCR was performed to quantify Lrp2 mRNA abundance in the kidney using SsoFast™ EvaGreen® Supermix kits (Cat # 172-5204 ...
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bioRxiv - Molecular Biology 2024Quote: ... HMGA2 fibroid (HMGA2F) expression was determined by RT-qPCR using the iScript Advanced cDNA Synthesis Kit (BioRad, #1725037) for the reverse transcription and HMGA2 PrimePCR primer (BioRad ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 1000 ng of total purified RNA following iScript cDNA synthesis kit (1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA was used in generation of cDNA using a reverse transcription kit (Bio-Rad, Hercules, CA) with gene-specific anti-sense primers ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 µl sample lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was assessed using the BCA Protein Assay Kit (Pierce) or the RC DC Protein Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... Molarity of the pool was quantified by qPCR using KAPA Library Quantification Kit and the CFX96 qPCR system (Biorad) before sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant were transferred to fresh tube and solubilized protein was measured using a DC protein assay kit (Bio-Rad). For immunoblotting an appropriate volume of 1 x Laemmli (Bio-rad ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
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bioRxiv - Immunology 2021Quote: ... Approximately 400 pg of RNA was subjected to reverse transcription using the iScript RT kit (BioRad, Inc., Hercules, CA). Resulting cDNA products were included with 50nM VH1-VH6 specific primers and 250nM Ca ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 0.5 to 1μg RNA using SensiFast cDNA synthesis kit and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Biorad) (refer Purushothaman et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNAs were extracted by the Aurum ™ Total RNA mini kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of total RNA was reverse transcribed with random primers using an iScript gClear cDNA synthesis kit (BioRad) and including a no-RT and controls for each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs was prepared using RevertAid First Strand cDNA Synthesis Kit (Thermoscientific) according to the manufacturer’s recommendation to perform qRT-PCR (BioRad) (Sup Table 1) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were quantified for protein concentrations using the Bio-Rad DC™ Protein Assay kit (Cat.5000112, BioRad) and loaded to a 10% SDS-PAGE gel ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The detection of the proteins on the membrane was performed using the Clarity Western ECL Substrate kit (Bio-rad). The signals were captured using the Alliance Mini (UVITEC Cambridge ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from REF subclones 2c and 11b using the Aurum Total RNA Mini Kit (Bio-rad) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The reverse transcription is carried out with 0.5 μg of RNA with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the DC Protein assay Kit (Bio-Rad). 25 µg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was then extracted using the Aurum Total RNA Mini Kit for Fatty and Fibrous Tissue (Bio-Rad). Reverse transcription reactions were performed using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse Transcription cDNA synthesis reactions were performed on 0.2 μg −2 μg total RNA with iScript cDNA synthesis kit (BioRad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using the Bradford method with the Quick Start™ Bradford Protein Assay Kit (#5000202, BIORAD) according to the manufacturer’s instruction34 ...
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bioRxiv - Microbiology 2019Quote: ... each gene segment was PCR amplified (100μl total reaction) using a gene specific primer set with iProof High Fidelity PCR kit (BioRad), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized by reverse transcription from isolated RNA using the iScript cDNA synthesis kit (Bio-Rad, 170–8891). Each 20 µl reaction followed the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was done using random primers for circRNA experiments from iScript™ Select cDNA Synthesis Kit (Bio-Rad). Fast Start Universal SYBR Green Master Mix (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad, 170-4270). After blocking with 5 % fat-free milk in PBS-Tween 20 ...
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bioRxiv - Genetics 2021Quote: ... Chemiluminescence detection was performed according to the manufacturer’s instructions (Amersham ECL Western Blotting Detection Reagent kit) followed by exposure using Chemidoc gel-documentation system (BioRad). For imaging using Li-Cor ...
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bioRxiv - Genetics 2021Quote: ... normalized to 10ng/μL and then reverse transcribed into cDNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The cDNA was used as template for the droplet digital PCR (ddPCR ...