Labshake search
Citations for Bio-Rad :
3951 - 4000 of 4847 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA by using an iScript kit (#1708891, Bio-Rad) on a Mastercycler (Eppendorf) ...
-
bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
-
bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation using the Gene Pulser Xcell (1700 V, 25 µF, 200 Ω, 1 pulse, Biorad) in C2989 5alpha electrocompetent bacteria (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were run on a 1% agarose gel and imaged using a Gel Doc XR+ (Bio-Rad).
-
bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 µg of RNA per sample using the iScript cDNA Synthesis Kit (Bio-Rad) or iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
-
bioRxiv - Immunology 2023Quote: ... by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) on a real time thermal cycler CFX96 (Biorad).
-
bioRxiv - Neuroscience 2023Quote: ... and 1 μg of RNA was reverse-transcribed (RT) to cDNA using iScript cDNA synthesis kit (Bio-Rad) with oligo-dT primers for poly-A mRNA selection ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
-
bioRxiv - Microbiology 2023Quote: ... diluted 1:5000 in TBE for 30 minutes while shaking and imaged on Gel Doc XR+(Bio-Rad). Full-length bands for at each time point were calculated by densitometry using Image Lab software (Bio-Rad) ...
-
bioRxiv - Plant Biology 2024Quote: ... Purified RNA (1 μg) was used for reverse transcription using the iScript™ cDNA Synthesis Kit (BIO-RAD). RT-qPCR was performed using SYBR green (ThermoScientific ...
-
bioRxiv - Microbiology 2024Quote: ... Screens were analyzed using a Molecular Imager FX and the Quantity One 1-D Analysis Software (Bio-Rad).
-
bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reverse transcribed to cDNA ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
-
bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
-
bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reversibly transcribed to cDNA through reverse transcriptase PCR.
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
-
bioRxiv - Biochemistry 2024Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on the 4-20% Criterion TGX Stain-Free Precast gels (BioRad) in Tris/Glycine/SDS running buffer (BioRad ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
-
bioRxiv - Plant Biology 2020Quote: ... Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1) and SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR system (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
-
bioRxiv - Cell Biology 2020Quote: All Rab34 used in steady-state assays was treated with AG 1-X8 anion exchange resin (Bio-Rad, 1401441) immediately before use to remove bound GDP ...
-
bioRxiv - Genetics 2021Quote: ... Hydrolysis probe ddPCRs (20 μl volumes) consisted of 1 x ddPCR Supermix for probes without dUTP (Bio-Rad Laboratories), virus-specific primers (HHV-6B POL F and HHV-6B POL R ...
-
bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
-
bioRxiv - Genetics 2022Quote: ... then 1-5ng added to a PCR mix containing 11 μl 2x ddPCR Supermix for Probe (no dUTP, BioRad), 1.1 μl 20x DMBT1 probe assay (FAM-labelled) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Plugs were loaded on 0.8% agarose gel in 1×TAE buffer (Certified Megabase agarose, Bio-Rad, ref# 161-3109) and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... Transformation was done chemically with heat shock at 42 °C for 30 s or by electroporation with 0.1 cm gap cuvettes at 1.8 kV for 1 s (Gene Pulser, Bio-Rad), depending on the competent cells required ...
-
bioRxiv - Microbiology 2019Quote: ... The integrity of the purified DNA template was assessed by conventional agarose gel (1% [wt/vol] electrophoresis (Bio-Rad), and the purified DNA samples were stored at 4 °C until further use.
-
bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
-
bioRxiv - Microbiology 2019Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in approximately 10 μl M9 medium and spotted onto 1% low-fluorescence agarose pads (Bio-Rad) prepared in M9 medium and covered with a no1.5 glass coverslip ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Reverse transcription reactions were performed from 1 μg RNA in 20 μL reactions using iScript reverse transcriptase (Bio-Rad). qPCR reactions were prepared in triplicate in a final volume of 10 μL using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
-
bioRxiv - Immunology 2019Quote: Samples were diluted 1 in 3 in HBSPE and run through Bio-Gel® P-30 (Bio-Rad, UK) polyacrylamide gel spin columns to minimise non-specific binding ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Biorad) was applied for 1 h at RT and blots were developed with Carity™ Western ECL Substrate (Biorad).
-
bioRxiv - Developmental Biology 2020Quote: Antibodies used in this study are specific to sheep GFP (1:500; AbD Serotec, Bio-Rad, Hercules, CA, USA), chick GFP (1:500 ...
-
bioRxiv - Genomics 2021Quote: ... PFGE was performed using 1% agarose gel in 0.5X TBE (Thermo-Fisher) in a CHEF Master XA 170-3670 system (BioRad) with the following parameters ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1 mM DTT) and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Biorad, 1704150EDU). Non-specific binding sites were blocked for 1 hour with 5% dry milk or BSA in TBS-Tween 0.1% while the primary antibody (1/1000 in 1% BSA TBS-Tween 0.1% ...
-
bioRxiv - Cell Biology 2021Quote: ... RNA (1 μg) was reverse transcribed into cDNA using iScript Reverse Transcription Supermix Kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared to the control gene 18S were determined by quantitative real-time PCR using SYBER Green PCR Supermix (Bio-Rad ...
-
bioRxiv - Bioengineering 2021Quote: Equivalent number of T cells (1×10e6) were lysed in Laemmli Sample Buffer with β-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore ...
-
bioRxiv - Bioengineering 2021Quote: ... Amplifications were performed in a 20 μL reaction containing 1 × ddPCR Supermix for Probes (No dUTP),(Bio-Rad, 1863024), 250 nM of probes and 900 nM of primers and 8 μL EV DNA template ...
-
bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
-
bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and exposed to X-ray film (Kodak) or the ChemiDoc Imaging system (Biorad) after incubation with Thermo Scientific Pierce ECL or Clarity ECL (Biorad).
-
bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
-
bioRxiv - Biochemistry 2021Quote: Reaction products were analysed by 20% denaturing polyacrylamide gel electrophoresis (made from 40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...