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Citations for Bio-Rad :
351 - 400 of 8843 citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of the mixed cell suspension was chilled on ice for 5-10 minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Immunology 2024Quote: ... Proteins were denatured at 94°C for 5 min before separation on SDS-PAGE (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels were used (Bio-Rad Laboratories)) ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... and boiled for 5 min before loading into a 12% or 4-20% acrylamide pre-cast SDS- PAGE gels (Bio-Rad, 4561045 or 4568094) for electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 hours followed by 20 μM Nigericin for 3 hours (ICT9146, Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... mixed 3:1 with 4x Laemmli buffer (Biorad, cat. no. 1610747) containing β-mercaptoethanol and heated at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... 30μg total protein from tissue samples with 4X Laemmli buffer was heat denatured at 99°C for 5 minutes and ran on resolving SDS PAGE gel (Biorad 4-20% precast gel #4561096). Sperm protein samples were prepared using a low volume of lysis buffer (∼100) ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Neuroscience 2021Quote: ... All Blue Prestained Protein Standards (1-3 µl; BioRad Laboratories, Hercules, CA) were used to identify band molecular weights ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-Drosophila AMPK1/2 (BioRad, 1:1000), rabbit anti-diphosphorylated ERK (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... ERK1/2 (1:1000, Bio-Rad Cat# MCA4695T), horseradish peroxidase-conjugated anti-rabbit (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1% v/v 2-mercaptoethanol (Bio-Rad) 48 hours after treatment (tetracycline-induced overexpression or doxycycline-induced knockdown) ...
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... 4 μl of the eluate was analyzed by 4-20% SDS PAGE (Biorad) and silver staining ...
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bioRxiv - Plant Biology 2024Quote: ... 4× Laemmli buffer and 4-20% SDS-PAGE gels were purchased from BioRad.
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bioRxiv - Genetics 2024Quote: ... crosses with gRNA-expressing males (GuideA.1-2; GuideB.1-2) was extracted using an adapted Chelex 100 Resin (Bio-Rad) protocol ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated together at RT for 5 min in the presence and absence of 5 mM 2-OG prior to loading on the analytical ENrich 650 column (BioRad Laboratories, Inc., Hecules, USA). The column was equilibrated with 50 mM Tris/HCl and 150 mM NaCl and supplemented with 5 mM 2-oxoglutarate when the complexes were preincubated in the presence of 5 mM 2-oxoglutarate and the applied proteins isocratic eluted with the respective buffer with a flow rate of 1 ml min-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were diluted 1:4 with Laemmli sample buffer (Bio-Rad), incubated for 95°C (5min) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were diluted 1:1 with 2× Laemmli buffer (Bio-Rad) supplemented with 54 mg ml-1 DTT ...
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bioRxiv - Bioengineering 2024Quote: ... and interleukin 4 (IL-4)) cytokines run on a Bio-Plex 200 reader (BioRad). Data was collected from only two donors for RANTES ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-tubulin YL1/2 (dilution 1:50; Biorad) and guinea-pig anti-Ana1 (dilution 1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... 4–20% gel (Bio-Rad) using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 0.8 × 4 cm (Bio-Rad). The column was washed with 10 column volumes (c.v. ...
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bioRxiv - Cell Biology 2020Quote: ... 4-20% TGX gels (BioRad) were used ...
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bioRxiv - Cell Biology 2021Quote: ... 4-20% gradient (Bio-Rad,); and then proteins were transferred to PVDF membranes ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-15% (Bio-Rad #4561085), with 1X Tris/Glycine/SDS Buffer (Bio-Rad #1610732) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-12% acrylamide gels (BioRad) were loaded with protein samples ...
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Cell Biology 2022Quote: ... Ampholytes (BioLytes pH 3–10, BioRad, Germany) were added to a total volume of 350 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.2% ampholytes pH 3-10 (BioRad) and added to 7 cm ReadyStrip IPG Strips with a linear pH range of 3-10 (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: FLICA™ 660 Caspase-3/7 (BIORAD) was used to measure apoptosis according to manufacturers.
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bioRxiv - Neuroscience 2024Quote: ... and embedded in 3% agar (Bio-Rad). Sections were cut at 60 µm on a vibratome and mounted on slides ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were run on 4–15% or 4-20% Mini-protean TGX gels (Bio-Rad) and transferred onto ImmobilonTM membranes (MilliporeSigma ...