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Citations for Bio-Rad :
351 - 400 of 3491 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-DYKDDDDK tag primary antibodies (Wako, 1E6, 1:5,000 dilution) combined with anti-mouse-HRP secondary antibodies (Bio-Rad, #1706516, 1:5,000 dilution). SigA proteins were detected using anti-SigA85 (1:10,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-CD8 (Biorad:MCA1226GA) 10 µg ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-Mouse HRP (BioRad) was used as a secondary detection antibody and immunoblots were developed with Forte HRP substrate (Millipore ...
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bioRxiv - Immunology 2019Quote: ... and mouse serum (BioRad). Blocking and subsequent surface staining was performed using PBS containing 2 mM EDTA ...
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bioRxiv - Immunology 2020Quote: ... and mouse serum (BioRad). Blocking and subsequent surface staining was performed using PBS containing 2 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... Mouse (Biorad, 10025637, qMmuCED0044924).
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bioRxiv - Cell Biology 2023Quote: ... WIPI2 (BioRad, MCA5780GA, mouse), AMBRA1 (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary α-mouse (BioRad) and α-rabbit (BioRad ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Cell Biology 2019Quote: ... blots were incubated with 1: 5000 dilution of HRP-conjugated anti-mouse/ rabbit IgG secondary antibodies (Bio-Rad) in 1% milk-TBST for another hour ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
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bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).
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bioRxiv - Genetics 2019Quote: ... followed by an anti-mouse (Abliance; 1/1,000) secondary antibody and then the Clarity Western ECL reagent (BioRad). Densitometric analysis was performed on non-saturated signals using the Image Lab™ software (BioRad).
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... The membrane was incubated with a primary mouse anti-pig IL-18 antibody (diluted 1:500, Bio-Rad) or anti-β-actin antibody (diluted 1:500 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated with Goat Anti-Mouse IgG (Heavy + Light)-HRP conjugate (170-6516, 1:1,000; Bio-Rad) overnight and following PBS-T washing detected using the LAS-500 Imaging System (General Electric).
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Neuroscience 2022Quote: Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6516,) and Goat anti-rabbit Igg (H+L ...
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bioRxiv - Immunology 2022Quote: ... supernatants were diluted at 1:4 and measured by Bio-Plex Pro mouse IFN-γ immunoassay (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with 1:10,000 dilution of anti-mousehorseradish peroxidase (anti-mouse-HRP) antibody (Bio-Rad) at room temperature for 1 h and washed again three times in TBST ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
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bioRxiv - Immunology 2023Quote: Plasma from control and infected mice was used at a 1:2 and 1:4 dilution with the Bio-Plex Pro Mouse Cytokine 23 Plex Immunoassay (Bio-Rad Laboratories, Inc., CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Mouse plasma was analysed using the mouse 23-plex cytokine panel (Bio-Rad) as specified by the manufacturer and contained the following targets ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Glucagon-like peptide 1 (GLP-1) and Adiponectin were measured using quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) and Bio-Plex Pro Mouse Diabetes Adiponectin assay #171F7002M (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was then washed thrice for 5 mins in PBS-Tween 20 before incubation with an HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the membrane was incubated with HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at 25°C before washing it six more times ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by incubation with a secondary HRP-conjugated anti-rabbit or anti-mouse antibody (1:2000, BioRad), depending on the origin of the primary antibody ...
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bioRxiv - Genetics 2021Quote: ... Membranes were washed and probed with secondary antibody (1:5000, anti-Rabbit-HRP, 1706515; anti-Mouse-HRP 1706516, BioRad) in blocking solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: rabbit anti-mouse collagen-IV (Bio-Rad Company, cat.# 2150-1470, 1:300), rat anti-mouse CD45 (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were the following: HRP-conjugated goat anti-mouse IgG (1:10000, Bio-Rad, 170-6516); HRP-conjugated goat anti-rabbit IgG (1:10000 ...
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bioRxiv - Physiology 2023Quote: After washing with TBS-T 0.1% membranes were incubated with goat anti-mouse (1:1000, Bio-Rad, Madrid, Spain), goat anti-rabbit (1:1000 ...
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:10,000, 52200336 and 52200341, Kirkegaard & Perry Laboratories) and detected by enhanced chemiluminescence using a Chemidoc (BioRad) gel imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with the primary antibodies and developed with a horseradish peroxidase-conjugated goat anti-mouse (BioRad, 1706516, 1:5,000), anti-rabbit (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... acidified to pH3–4 by adding appropriate amount of TFA and subjected to HPLC purification using a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA). The S-sulfonated FAM237A was eluted from the C4 reverse-phase column by an acidic acetonitrile gradient composed of the acidic solvent A (0.1% aqueous TFA ...
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bioRxiv - Biochemistry 2023Quote: ... and the S-sulfonated FAM237B was eluted from a C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad, Hercules, CA, USA) by an acidic acetonitrile gradient ...
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bioRxiv - Microbiology 2024Quote: ... and HopAM1 and C-terminal Flag-tagged IpaH4 were generated by PCR amplification using primers containing Flag sequences and flanking SacII / PacI off pIB/V5-His templates using iProof DNA polymerase (Bio-Rad; Table S4) and cloned into the pDGOpIE2 vector ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-mouse HRP (Biorad). For immunocytochemistry ...