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Citations for Bio-Rad :
351 - 400 of 3233 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The PEI pellet was dissolved in 0.4 M ammonium sulfate buffer and applied onto a column packed with Macro-Prep High Q resin (Biorad). The eluted peak was then precipitated with 4 M ammonium sulfate and the re-dissolved pellet applied onto an 8WG16 antibody column ...
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bioRxiv - Plant Biology 2022Quote: ... The synthesized crTCA cycle genes in pUC57 were used as template DNA with iProof High-Fidelity DNA polymerase (BioRad). The PCR products were gel purified ...
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bioRxiv - Microbiology 2022Quote: ... Acetate was measured by high performance liquid chromatography (HPLC) with an Aminex HPX-87H column (Bio-Rad, Hercules, CA), coupled to an RI and UV detectors (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Acetate was measured by high performance liquid chromatography (HPLC) with an Aminex HPX-87H column (Bio-Rad, Hercules, CA), coupled to RI and UV detectors (Waters ...
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bioRxiv - Genomics 2023Quote: ... to ensure high integrity and later analyzed with pulse-field gel electrophoresis on CHEF DR-II (BioRad, CA, USA) to ensure the size range between 20 kb to 100 kb ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... for high-sensitivity chemiluminescent protein detection with Uvitec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...
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bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Genetics 2022Quote: ... Proteins were separated by SDS-PAGE with 2-mercaptoethanol as a reducing agent using criterion sized 4-15% gradient tris-glycine for broad range of molecular weights greater than 30 kDa or 16.5% tris-tricine for high resolution of smaller proteins (Bio-Rad). Proteins were transferred to nitrocellulose membranes and probed with antibodies [commercially obtained except for ATF6 [63] ...
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bioRxiv - Genomics 2019Quote: Two hundred clonal adult male worms were used to prepare high molecular weight genomic DNA using CHEF Genomic DNA Plug Kits (BioRad). The DNA was quantified on a FEMTO Pulse® ...
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bioRxiv - Synthetic Biology 2020Quote: ... Resulting images were adjusted uniformly (‘High’ set to 40,000) to improve visibility of growth (Bio-Rad Image Lab™ Software). Figures utilizing these images (Supplementary Figs ...
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bioRxiv - Genetics 2020Quote: ... Glycated haemoglobin (HbA1c) was estimated by high-performance liquid chromatography using a Variant™ machine (Bio-Rad, Hercules, CA, USA). Serum insulin concentration was estimated using an enzyme-linked immunosorbent assay (Dako ...
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bioRxiv - Genomics 2021Quote: ... We designed and purchased the hygro-AI cassette at Genescript and further amplified it by PCR using Iproof high fidelity DNA polymerase (BioRad). The 5’-LTR-gagpol and the 3’-LTR segments were obtained by PCR amplification from the pOY1 template [40] ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were hybridised with an appropriate DNA probe— as indicated in figure legends and Table S2—radiolabelled with P32 prepared via random priming using the High Prime kit (BioRad). Pre-hybridisation solution was discarded ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA retro-transcription was performed using a High-Capacity cDNA Reverse Transcription Kit on a MyCycler (Bio-Rad, Hampton, NH). Next ...
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bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
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bioRxiv - Microbiology 2023Quote: ... high-performance liquid chromatograph (HPLC) using an Aminex HPX-87H analytical column (part no. 1250140, Bio-Rad, Hercules, CA, USA) protected by ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-12 (10 ng/mL)] or transferred to a high-binding 96-well plate pre-coated with indicated amounts of control or TAG72 antigen (BioRad). Reagents and buffers for flow cytometry processing were pre-chilled on ice unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad). Membranes were blocked with 10% skim milk in TBS-T for 2h with gentle agitation at RT ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The other half of the SDS-PAGE gel with the high-MW bands was stained with Bio-Safe™ Coomassie Stain (Biorad). The intensity of Western-blot bands was quantified by the ImageJ software (50) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was produced using the High Capacity RNA-to-cDNA Kit (Applied Biosystem) and the cDNA was pre-amplified with SsoAdvanced PreAmp Supermix (Bio-Rad) using primers from Integrated DNA Technologies or Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... Phusion high fidelity polymerase chain reaction (PCR) was used to amplify the gene with a T100 Thermal Cycler (BioRad, Hercules, CA). Each PCR reaction contained in a final volume of 50 µl with 1 x Phusion Master Mix containing HF buffer (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... MenD mutants were generated using the pYUB28b-menD construct and oligonucleotide primers (Supplementary Table 2, Integrated DNA Technologies) with iProofTM high-fidelity DNA polymerase (Bio-Rad). The PCR products were then treated with DpnI and ligated using T4 DNA ligase (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... ∼500bp fragments of sRNA factors were amplified from cDNA of ISE6 cells using primers listed in Table S1 using iProof High- Fidelity Master Mix (Bio-Rad) and the amplicons were treated with XhoI to insert them in the XhoI site of pLitmus28i (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Plant Biology 2021Quote: ... Quality and quantity of RNA samples were assessed using the Bio-Rad Experion analyser and Experion RNA high-sense analysis kit (Bio-Rad). Total RNA from each biological replicate was amplified using the MessageAmp II aRNA amplification kit (Ambion ...
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bioRxiv - Bioengineering 2021Quote: ... and were scanned and monitored using high-performance liquid chromatography (HPLC) (Waters, 2707, NL) equipped with an Aminex HPX-87H column (BioRad, USA) column ...
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bioRxiv - Plant Biology 2019Quote: ... subsequently the powder was dissolved and desalted as above then incubated with Macro-Prep High S cation exchange media (Bio-Rad). The supernatant and five water washes were again collected and stored ...
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bioRxiv - Microbiology 2019Quote: ... SCFA concentrations were measured with a CBM-20A high performance liquid chromatography (HPLC) system equipped with an Aminex HPX-87P column (300 x 7.8 mm, BioRad, California, USA), maintained at 60 °C ...
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bioRxiv - Microbiology 2020Quote: Acetate was measured with a high-performance liquid chromatograph (HPLC) (Waters, 2707, NL) equipped with an Aminex HPX-87H column (BioRad, USA). Samples were eluted using H3PO4 (1.5 mmol L-1 ...
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bioRxiv - Bioengineering 2022Quote: ... acetate concentrations were measured using a high-performance liquid chromatograph (HPLC) equipped with an Aminex HPX-87H column (BioRad, United States) maintained at 59°C and coupled to an ultraviolet detector at 210 nm (Waters United States) ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a nitrocellulose membrane using the High Molecular Weight protocol on the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% milk in 0.05% Tween 20 in PBS for 1h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blotted on nitrocellulose membranes and transfer was carried out by using the “high molecular weight” program of the Trans-Blot Turbo System (Bio-Rad). The membranes were blocked in a blocking solution (5% skim milk ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from promastigotes or lesion-derived amastigotes and converted into cDNA using a high-capacity reverse transcription kit (Bio-Rad), followed by qPCR using primers targeting EPCT or α-tubulin coding sequences ...
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bioRxiv - Neuroscience 2023Quote: ... F0 fish were screened by high resolution melt (HRM) analysis using gene-specific primers (Table 2) with Precision melt supermix (Biorad 1725112) according to the manufacturer’s protocol in a BioRad CFX RT-PCR thermocycler ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µL of the bisulfite converted DNA was combined with 10 µL of Precision Melt Supermix for High Resolution Melt Analysis (BioRad 1725112), 2 µL of Forward and Reverse primers (2 µM stock ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing ADDoCoV were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: Concentrations of acetate were measured from the bulk liquid phases using a high-performance liquid chromatograph (HPLC) equipped with UV and RI detectors (BioRad, USA), after sample filtration on 0.2 μm filters ...
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bioRxiv - Bioengineering 2023Quote: ... followed by an additional wash and resuspension in Assay Buffer for readout by high-throughput cytometry (Bio-Rad ZE5 cell analyzer). Results were decoded using Nomic software ...