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Citations for Bio-Rad :
351 - 400 of 3005 citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... This was then run for 35 min at 200 V on a 4-20% polyacrylamide gel (MiniPROTEAN TGX, BioRad) in 1x SDS running buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Bioengineering 2019Quote: ... The anti-SSTR2 mAb was purified using our two-step antibody purification protocol by the NGC system (Bio-Rad, Hercules, CA) equipped with Protein A column and ion exchange column.(34 ...
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bioRxiv - Molecular Biology 2019Quote: The SPR studies to determine conformation binding of the human chimeric 1A8 and 7C8 mAbs were carried out with the ProteOn XPR36 protein interaction array system (Bio-Rad) based on SPR technology ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B, BioRad).
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bioRxiv - Physiology 2023Quote: ... and goat anti-rat PE (50x stock concentration not reported, STAR73, Bio-Rad) diluted in PTxwH buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and the volumes corresponding to 35 μg were loaded onto TGX Stain-Free FastCast 12% polyacrylamide gels (Bio-Rad, USA) for electrophoretic separation ...
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was carried out in triplicates and was performed at a 2-step reaction with 95°C denaturation and 56°C annealing and extension for 35 cycles on a CFX96 Real-Time System (BIORAD). Relative quantification of target genes was determined using Bio-Rad CFX manager 3.1 software ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... Ten microliters of each amplicon were separated on 2% agarose gel in 1X TAE buffer for 35 min at 135 volts and the amplified fragment detected using a GelDoc imager (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and mouse monoclonal anti-β-actin (Cat # VMA00048, Bio-Rad, Hercules, CA, USA). Next ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Physiology 2021Quote: ... adipose tissues were stained with rat anti-F4/80 primary antibody (Bio-Rad, #MCA497B) and goat anti-rat HRP polymer (Cell IDX ...
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bioRxiv - Plant Biology 2021Quote: ... were coated with 2 μg of plasmid DNA and introduced into plants laid on a 35-mm polystyrene dish using a helium gun device (PDS-1000/He, Bio-Rad) according to the manufacturer’s instructions (vacuum ...
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bioRxiv - Immunology 2020Quote: ... Measurements and analyses of secreted cytokines from a murine 35-plex kit were performed using a multiplex bead-based technology (Luminex) assay with a Bio-Plex 200 instrument (Bio-Rad) after 2-fold dilution of supernatants.
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bioRxiv - Cancer Biology 2021Quote: ... MA) and 35 μg of protein were loaded and size-fractionated by SDS-polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, CA). Gels were then transferred to PVDF membranes ...
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bioRxiv - Microbiology 2023Quote: ... and the gel was run at 180 V for about 35 minutes (Mini-PROTEAN electrophoresis cell and PowerPac power supply, Bio-rad). Coomassie brilliant blue (Bio-Safe Coomassie stain ...
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bioRxiv - Plant Biology 2023Quote: ... during 35 minutes at 200 V (Mini-PROTEAN® Tetra Cell, 4-Gel System, Bio-Rad laboratories Inc, Hercules, CA, USA) and were electroblotted onto 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred to a 0.22 µm PVDF membrane by blotting at 24 V for 35 min using a Trans-Blot TurboTM system (BioRad, Feldkirchen, Germany). The membrane containing the proteins was blocked in 5% BSA in TBST at RT for 1 h to avoid unspecific binding of the antibodies ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Fragments amplified using the FastGene Optima HotStart Ready Mix (Nippon Genetics) over 35 cycles in a C1000 Thermal Cycler (Bio-Rad) were subjected to agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD11b (Clone OX-42) mouse monoclonal antibody (Cat. No.: MCA275G; BioRad (formerly serotec)) ...
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bioRxiv - Immunology 2022Quote: ... The HN protein was detected by a mouse monoclonal antibody 8H2 (MCA2822, Bio-Rad). The membranes were then washed with PBST on a shaker 3 times (10 min at RT each time ...
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bioRxiv - Bioengineering 2020Quote: ... Anti-adalimumab/TNFα monoclonal antibody (HCA207) and anti-infliximab (HCA213) were obtained from BioRad. Anti-SARS-COV-2 antibodies 47D11 and 49F1 were produced in HEK-293T cells and purified using Protein-A affinity chromatography as described previously44 ...
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bioRxiv - Neuroscience 2019Quote: ... and run at 240 V for 35 minutes prior to semi-dry transfer to a 0.45 µm thick nitrocellulose membrane (Bio-Rad, Cat#: 1620115) at 16 V for 40 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CD8 (2 µg/rat dissolved in saline solution, clone OX-8, Bio-Rad #MCA48G) or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution ...
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bioRxiv - Microbiology 2020Quote: ... while the HN protein was detected by a mouse monoclonal antibody 8H2 (MCA2822, Bio-Rad). The membranes were then washed with PBST on a shaker 3 times (10 min at RT each time ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 20,000 x g for 20 min and the cleared lysate was passed through a 0.45-µm filter and loaded onto a XK-26/20 column (Cytiva) with 30-35 ml ceramic hydroxyapatite (Bio-Rad, cat no. 1582000). PlyCBWT was eluted from the column with three column volumes of 1 M sodium phosphate buffer (pH 7.2) ...
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bioRxiv - Cancer Biology 2024Quote: ... Dharmacon, Cambridge, UK) or siRNAs targeting MYC (D-003282-14 & D-003282-35, Dharmacon) using siLentFect transfection reagent (1703362, Biorad, Hercules, CA, USA) (see Table S4 for siRNA sequences) ...
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bioRxiv - Cell Biology 2019Quote: Sections of mice peritoneum were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), rabbit anti-mouse CD38 antibody (bs-0979R ...
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bioRxiv - Bioengineering 2023Quote: ... Gene analysis of TREM2 (PrimePCR™ SYBR® Green Assay: TREM2 for Rat; BioRad, CA, USA) was performed following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and stained with rat anti-mouse CD68 primary antibody (clone FA-11; Bio-Rad, Hercules, CA) followed by biotinylated rabbit anti-rat IgG secondary antibody (Vector Laboratories ...
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bioRxiv - Biochemistry 2021Quote: The purified SdGA was analyzed by SDS-PAGE using 12% (w/v) gels as described by Laemmli [35] with a Bio-Rad Mini Protean system (Bio-Rad, Hercules CA, USA). Gels were stained by Coomassie Blue and/or electro-blotted onto nitrocellulose membranes (HybondTM-ECLTM ...
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bioRxiv - Neuroscience 2022Quote: ... was used for quantitative real time PCR with commercially available rat Magel2 and Gapdh primers (Bio-Rad, USA ...
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bioRxiv - Neuroscience 2024Quote: ... For analysis the Bio-Flex Pro™ Rat Cytokines Assay (Bio-Rad, 24-plex panel #171-K1001M) was used on a Bio-Plex® MAGPRIX™ multiplex reader and analysed with Bio-Plex Analysis software (Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... AP-conjugated anti-DIG monoclonal antibody was used in combination with Immun-Star substrates (Bio-Rad Laboratories). Signals were captured by ChemiDoc (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2020Quote: 10 μm sections of mouse tumors were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Physiology 2021Quote: Quantitative PCR for rat FXR and reference gene GAPDH was performed using SsoAdvanced Unviersal SYBR Green Supermix (BioRad) in the ViiA 7 Real-Team PCR System (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression levels of 85 genes were assessed using the PrimePCR Antibacterial Response (SAB Target List) R384 rat plate (Catalog #10047065; Bio-Rad) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...