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Citations for Bio-Rad :
351 - 400 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The purified RNA was separated on a 1.7% agarose–formaldehyde denaturing gel and was then transferred to a nylon membrane (Hybond-N+, Cytiva) in 10× SSC buffer using a model 785 vacuum blotter (Bio-Rad). RNA was cross-linked to the membrane using an ultraviolet (UV ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... at which point the crushed gel could be transferred from the tube lid to a DNA spin column (Freeze ‘N’ Squeeze, Bio-Rad) with tweezers ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Cancer Biology 2021Quote: ... XP2OS cells were transfected with 200 ng of plasmid containing the XPA VUS of interest or wild-type XPA as well as the FM-HCR reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad Laboratories #165-2670). Plate electroporation was performed at 260 V ...
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bioRxiv - Molecular Biology 2023Quote: ... Individuals with a positive HIV-1 RNA test and a negative HIV-1 ELISA test (at the time of first RNA positive test) and an evolving Western blot (GS HIV Type 1 Western blot, Bio-Rad, Redmond, WA, USA) pattern indicative of a recent HIV-1 infection were enrolled ...
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bioRxiv - Neuroscience 2024Quote: ... the expected sizes of bands (254 bp for wild type and 433 bp for mutant) were visualized with ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μL 5 N NaOH solution was added to and the absorbance was read at 405 nm with a reference filter of 620 nm by iMark Microplate Reader S/N 20255 (Bio-Rad, USA).
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bioRxiv - Synthetic Biology 2020Quote: ... Target bands were excised on a blue light transilluminator and purified with a Quantum Prep® Freeze ‘N Squeeze spin column (Bio-Rad). The eluate was further purified with a magnetic bead clean-up (Agencourt® AMPure® XP ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Physiology 2023Quote: ... in duplicates in 384-qPCR plates (n = 5 plates) and qPCR were performed with a Biorad instrument (CFX-384, Biorad, Hercules, USA). We used Recombination Activating Gene 1 (RAG1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... and human 18S genes using the iQ™ SYBER® Green Supermix (Bio-Rad, #1708880) with the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... For cytokine quantification Bio-Plex Pro Human Cytokine Screening Panel 48-plex (BioRad, USA; 12007283), Bio-Plex Pro Human Cytokine ICAM-1 (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... Alexa Fluor 488-labeled mouse anti-human CD81 antibody (MCA1847A488) was purchased from Bio-Rad. Alexa Fluor 555-labeled mouse anti-human EpCAM antibody (5488S ...
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bioRxiv - Microbiology 2024Quote: ... or with 0.1 µg/well of mouse anti-human IgG (CH2 domain) clone R10Z8E9 (BioRad) for Fc capture in carbonate buffer ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were then incubated with Human TruStain FcX and Purified mouse IgG (BioRad, PN PMP01X) for 10 minutes at room temperature and washed with Wash Buffer (1% FBS in PBS Solution) ...
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bioRxiv - Cell Biology 2024Quote: ... Relative expression of target gene levels were calculated according to the ΔΔCT method normalized to mGapdh levels and then wild-type mouse cohort using Bio-Rad CXF Maestro 2.3 software (Bio-Rad Laboratories Inc., Hercules, CA, U.S.).
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bioRxiv - Microbiology 2023Quote: ... was collected from each sample and transferred to 96-well ELISA plates for the measurement of the absorbance at 595 nm by using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Alfred Nobel Drive. Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
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bioRxiv - Molecular Biology 2024Quote: ... separated by gel electrophoresis and the band at ∼220bp excised using Freeze ’N Squeeze™ DNA Gel Extraction Spin Columns (Biorad cat#7326166). The DNA was further concentrated using Zymo DNA clean and concentrate columns (Zymo cat#D4014 ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Immunology 2020Quote: Supernatants were thawed and immediately analyzed with Bio-plex human cytokine assays (Bio-Rad Laboratories, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into K562s using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2024Quote: ... and human primers (listed below) using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The primers used are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: Luminex analysis was performed using the Bio-Plex Pro Human Cytokine 27-plex Assay (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The plasmid curing vector was introduced into the wild-type strains by electroporation (1.8 kV, 25 µFar, 200 Ω) using Gene Pulser Xcell™ electroporation system (Bio-Rad Laboratories Inc., California, USA) as described before15 ...
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bioRxiv - Systems Biology 2021Quote: ... which contained 200 nM of TSWV-N or thrips internal reference Actin gene primers in the SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Samples with missing and/or high (>35 ...
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bioRxiv - Microbiology 2023Quote: ... ddPCRs for each sample (n=50) were prepared in an initial volume of 72 µl comprising 37.5 µl EvaGreen Supermix (QX200; Bio-Rad, South Granville, NSW, Australia), 31.5 µl sterile water ...
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bioRxiv - Plant Biology 2024Quote: RNA samples resolved in denaturing polyacrylamide gels were transferred to positively charged nylon membranes (Cytiva, Hybond-N+, catalog no. 45-000-850) using a semi-dry Trans-Blot Transfer System (Bio-Rad, catalog No. 1703940) in 0.5X TBE at constant 20 V for 45 min ...
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bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...
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bioRxiv - Pathology 2024Quote: ... and cytokines were detected using the Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, #M5000031YV), including IL-1β ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Immunology 2024Quote: ... Approximately 600 RUs of anti-human IgG (Cytiva) were immobilized on a GLC Sensor Chip (Bio-Rad) using amine-coupling ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Supernatant cytokines were assayed using the Bio-Plex Human Cytokine 17-plex assay system (Biorad, Hercules CA) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The decorated DNA origami products were purified using agarose gel electrophoresis and Freeze N’ Squeeze DNA Gel Extraction Spin Columns (#7326165; Bio-Rad Laboratories, Hercules, CA, USA), immediately followed by dialysis with Folding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...