Labshake search
Citations for Bio-Rad :
3901 - 3950 of 4080 citations for 7 Trifluoromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were prepared at a concentration of 1 μg/μL and 15 μL of sample was loaded onto 4-15% gradient SDS-polyacrylamide gels (Bio-Rad Laboratories). Following the electrotransfer of proteins onto PVDF membranes ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
-
bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
-
bioRxiv - Plant Biology 2024Quote: ... and 1 µl of cDNA was used as a template for qRT-PCR using iQTM SYBR® Green Supermix (BIO-RAD, USA) on a CFX96TM Real-Time PCR machine (BIO-RAD ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 0.5 μL of forward and reverse primers (see Supplementary Table 1 for primer sequences) for the genes of interest were combined in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a CFX384 Real Time PCR Detection System using the following program ...
-
bioRxiv - Developmental Biology 2024Quote: ... see Supplementary Table 1 for primer sequences) and diluted to 20 μL with nuclease free water in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a Bio-Rad CFX384 Real Time PCR Detection System with the following program ...
-
bioRxiv - Immunology 2024Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
-
bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were incubated with fresh serum-free medium containing 10% CCK-8 for 1 hour at 37°C and then the absorbances at 450 nm were measured using a microplate reader (Bio-Rad, Hercules, USA).
-
bioRxiv - Cancer Biology 2021Quote: ... 1 µg DNA from each sample was transferred to a nitrocellulose membrane using the Bio-Dot SF microfiltration apparatus (Bio-Rad, #170-6542). The membrane was blocked in 5% non-fat milk in TBS with 0.1% Tween-20 ...
-
bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
-
bioRxiv - Evolutionary Biology 2020Quote: ... 0.08 of each M13 dye and 1 μL of the DNA template using a Bio-Rad thermocycler T100 (Bio-Rad, Pleasanton, CA, USA). PCR products were analyzed on an ABI 3500 genetic analyzer and sized against the LIZ500 internal standard (Applied Biosystems ...
-
bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
-
bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
-
bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
-
bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
-
bioRxiv - Biophysics 2019Quote: ... The relative amount of present HRP was detected by adding 60 µL/well DPBS + 1 mM CaCl2 and 20 µl/well HRP substrate (Bio-Rad, 170-5060). The plate was incubated for 10 minutes at room temperature before recording of luminescence on an EnVision plate reader (Perkin Elmer).
-
bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
-
bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and then subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent loading of total protein ...
-
bioRxiv - Immunology 2019Quote: ... cDNAs were synthesized from 1 μg total RNAs and qPCR were performed by using the iScript™ cDNA Synthesis Kit (BioRad, the Netherlands) and iQ™ SYBR Green Supermix (BioRad ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
-
bioRxiv - Immunology 2021Quote: ... Transcript expression was measured by qRT-PCR with the appropriate primers (Supplemental Table 1) using a Bio-Rad MyiQ™ Real-Time PCR Detection System (Bio-Rad Laboratories) to measure SYBR Green (IQ™ SYBR® Green Supermix ...
-
bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... cys free or AAA SNAREs were reconstituted at a 1:400 protein:lipid ratio of the same lipid compositions by dialysis in the presence of Bio-Beads (Bio-Rad Laboratories, Hercules, CA) into metal-free buffer ...
-
bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
-
bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription (RT) was performed with 1 μg of total RNA using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 172-5272). cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems ...
-
bioRxiv - Cell Biology 2021Quote: ... After three washes (5, 10, 15 minutes) the blot was incubated for 60 minutes with secondary Goat Anti-Rabbit-HRP (Bio-Rad, 1:7000) and Alexa 647 Goat-Anti-Mouse antibodies (Molecular Probes ...
-
bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% skimmed milk in TBST for 30 min at room temperature then incubated with primary antibodies overnight at 4°C and secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at room temperature before visualization by ECL Plus Western blotting detection reagent (Bio-Rad, Hercules, CA). Semi-quantification of the bands were performed using NIH Image J software.
-
bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
-
bioRxiv - Immunology 2022Quote: ... The affinity matrix was resuspended in 150 μl PB for the unbound and full plasma fraction and in 40 μl PB with a 1% blocking buffer background (Bio-Rad, The Netherlands) for the bound fractions ...
-
bioRxiv - Microbiology 2022Quote: ... were used as secondary antibodies for 1 hour at the room temperature and revealed with the addition of Clarity Western ECL reagent (Bio-Rad Laboratories Ltd). ChemiDoc MP Imaging System (Bio-Rad Laboratories Ltd ...
-
bioRxiv - Plant Biology 2021Quote: ... A second polyclonal antibody conjugated with alkaline phosphatase was used for immunodetection (1:1000) at wavelength 405 nm (BioRad Microplate Reader model 680) via the substrate p-nitrophenyl-phosphate ...
-
bioRxiv - Neuroscience 2020Quote: ... plates were coated with 100 μl of either 1 μg/ml of rh-TfR1-ECD (purified in-house) or liver purified ferritin (Bio-Rad, 4420-4804) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
-
bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... and α-TUBULIN (1:4000) were detected using SuperSignal™ West Pico or West Femto Chemiluminescent Substrate chemiluminescence in ChemiDoc MP imaging system (BioRad, Berkeley, USA). The densitometry analyses were performed using Bio-Lab Software ...
-
bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
-
bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
-
bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
-
bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
-
bioRxiv - Genomics 2019Quote: ... the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion™ automated electrophoresis system (Biorad Laboratories, Australia).
-
bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNAs were subjected to PCR analyses using specific primers (The primer sequences are listed in Table 1) on an amplification detection system (Bio-Rad, Hercules, USA). β-actin served as a housekeeping gene ...
-
bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).