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Citations for Bio-Rad :
3851 - 3900 of 4172 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and total RNAs were obtained according to the manufacturer’s protocol and then quantified and synthesized into cDNA using a iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA) for SALL4 expression or using a High-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... we synthetized 100ng of cDNA from immunoprecipitated and isolated polyribosome-associated RNA (see above for detailed procedure) as well as non-precipitated RNA (input) using the iScript cDNA synthesis kit (Bio-Rad, USA). cDNA was then preamplified using TaqMan assay following kit instructions46 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were reverse transcribed from 500 ng of total RNA in a 20 µL volume using iScript cDNA synthesis kit (Bio-Rad Laboratories). Real-time qPCR was carried out on a C1000™ Thermal Cycler (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of each RNA sample was converted into single-strand cDNA with random hexamers using an iScript™ Select cDNA Synthesis Kit (Bio-Rad). To validate fusion transcripts ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of RNA was used to generate cDNA with the iSCRIPT Clear kit (Bio-Rad, Hercules, CA, cat# 170-8891). For the qRT-PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative cDNA detection was performed via quantitative real-time PCR (RT-qPCR) with the same gene-specific primers using the iQ™ SYBR® Green Supermix Kit (BIORAD). Serially diluted genomic DNA was used as a quantification standard.
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Cell Biology 2021Quote: ... in accordance with the manufacturer’s protocol then performing qPCR using a Sangon Biotech microRNA qPCR kit with SYBR Green (B532461) in a Bio-Rad CFX96™ real-time PCR detection system (Bio-Rad) using the following thermocycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral or control antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: Bio-Plex assays for cytokines and chemokines were carried out in Bio-Plex 200 system using Bio-Plex Pro Mouse Cytokine Grp I Panel 23-Plex assay kit (Bio-Rad, M60009RDPD) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was subjected to SYBR Green qPCR using iTaq™ Universal SYBR Green Supermix kit from Bio-Rad (Hercules, CA, US) and primers specific to the 5’-end of tRFs and RNA linker ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram was reverse transcribed to generate first-strand complementary DNA (cDNA) using IScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR experiments were performed with 2.5 μl of a 1:20 dilution of first-strand cDNA and LightCycler 480 SYBR Green I Master mix ...
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bioRxiv - Plant Biology 2021Quote: ... and treated aliquots amounting to 10 ng RNA were used for cDNA synthesis (iScript™ cDNA Synthesis Kit, BioRad, Hercules, CA, USA). Quantitative real-time PCR was carried out using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from freshly isolated or cultured cells using the Aurum Total RNA Mini Kit (732-6820, Bio-Rad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with reagents included in the Efficient Chemiluminescence Kit (Vigorous, Beijing, China) and ChemiDoc XRS (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Aliquots of the samples were organized in a 96well PCR plate and reverse transcribed to cDNA using the iScriptTM Reverse Transcription Supermix kit and protocol (BioRad; Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal amounts of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane by Trans-Blot® TurboTM RTA Mini PVDF Transfer Kit (Biorad, USA). Later ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad) and stored at −20 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using 1 μl of total RNA from melanocytes or iridophores with iScript™ Advanced cDNA Synthesis Kit (#1725038, Bio-Rad) following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScript™ One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were quantified using the Kapa SYBR FAST Universal qPCR kit for Illumina sequencing on the CFX384 Real-Time PCR Detection System (Bio-Rad Laboratories). The libraries were diluted to 4 nM stocks and pooled equitably for sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was performed using 250 ng of DNase-treated RNA using the iScript cDNA synthesis kit (Bio-Rad Cat#1708890) and the resulting RT reactions were diluted 1:5 with nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatants were screened for the presence of 27 human cytokines and chemokines using the Bio-Plex Pro Human Cytokine Standard 27-Plex kit (Bio-Rad Laboratory) on a FLEXMAP 3D® analyzer (Luminex ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified and normalized PCR products were further pooled into a single library and quantified using qPCR (KAPA Library Quant Kit qPCR Mix, Bio-Rad iCycler). The final library was diluted to 4 nM and sequenced in an Illumina HiSeq 2500 Platform using a 2×250bp RapidRun kit for an average coverage of 160,000 paired reads per PCR product.
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bioRxiv - Immunology 2020Quote: ... Immunoblots were conducted using Mini-Protean TGX stain-free gels and Transfer-Blot Turbo RTA Transfer Kit reagents(Bio-Rad, Mississauga, Ontario). ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was estimated by nanodrop and 1 μg of RNA was used to synthesize cDNA with iScript cDNA synthesis kit™.(Biorad 1708890) qPCR was performed using Precision™ 2X qPCR master mix (Primerdesign ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 μg of RNA was reverse transcribed into cDNA using BUNiScriptTM cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 170-8891). For pancreas samples ...
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bioRxiv - Microbiology 2022Quote: ... The amplification kinetics of each product were checked at the end of each cycle by measuring the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed and stained gene expression slide was treated with a permeabilization enzyme specific for mouse tongue determined by the Spatial tissue optimization slide kit (10x Genomics, #1000191) for 24 minutes on the thermal cycler (Bio-Rad, 1851197). Synthesis of cDNA and second strand were performed using reverse transcriptase and second strand reaction (primer and enzyme) ...