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Citations for Bio-Rad :
3851 - 3900 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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bioRxiv - Cancer Biology 2023Quote: ... were thawed on ice for 20 min prior to addition of 1 μL of Gibson reaction and electroporation (1800 V) in a MicroPulser Electroporator (Biorad). 975 μL of pre-warmed recovery medium were added and the bacteria were incubated 37 °C at 250 rpm for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed on 1 µg of purified RNA using the iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA). For each quantitative real-time PCR reaction ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with Amersham ECL Western Blotting Detection Reagent (Global Life Science Solutions) for 1 min and imaged on a ChemiDoc Imaging System (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... Polysome profiling was carried out using a Piston Gradient Fractionator (Biocomp) with continuous monitoring at 254 nm using a Model EM-1 Econo UV detector (BioRad). Monosome and polysome fractions of approximately 400 μl were collected with a fraction collector (Gilson FC203B).
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... blots were incubated in 1-shot ECL solution (Kindle Biosciences LLC) and imaged with a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Genetics 2024Quote: ... followed by purification and size selection on a 1% agarose gel with 0.6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR was carried out using the PerfeCTa SYBR Green Fastmix (Quantabio, 95071) and gene-specific primers (Table 1) on CFX96 system (Biorad). Relative gene expression was determined using the ΔΔCt method.
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bioRxiv - Microbiology 2024Quote: ... 50 μl of M18 competent cells were mixed with 150 ng of plasmid DNA and electroporated using 1-mm cuvettes in a Gene Pulser Xcell (Biorad) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... incubated in a 1:1000 dilution of the primary antibody (either anti-V5 tag mouse monoclonal antibody, MCA1360, Bio-Rad, or anti-Softag1 SLAELLNAGLGGS mouse monoclonal antibody to detect the β’ subunit of E ...
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bioRxiv - Genomics 2024Quote: ... the plug slices were loaded into the appropriate wells of a 1% agarose gel (Pulsed Field Certified Agarose, BioRad-Laboratories). The gel was electrophoresed for 18 h in 0.5× TBE at an angle of 120° with initial and final switch times of 2 and 10 seconds respectively ...
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bioRxiv - Microbiology 2024Quote: ... Bound antibodies were detected using a 1:10,000 dilution of HRP-conjugated goat anti-rabbit secondary antibody (BioRad #170-6515) and an enhanced chemiluminescence detection kit (Millipore) ...
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bioRxiv - Immunology 2024Quote: ... 75 μg of protein per sample was mixed with 1× Laemmli sample buffer (cat. no. 1610747, BioRad, Hercules, CA, USA), resolved on an Anykd™ Criterion™ TGX™ Precast Midi Protein SDS-PAGE gel (cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Electrophoresis of the DNA origami was performed using 0.7 or 1% agarose gel (#161-3106; Bio-Rad Laboratories, CA, USA) and 0.5× Tris/Borate/EDTA buffer containing 5 mM MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... Immunoblotting was performed as described above in “Protein photocrosslinking and analysis” using anti-SNARE antibodies and Tidyblot (1:1000; BioRad) in 5% nonfat milk in TBST ...
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bioRxiv - Microbiology 2024Quote: ... and cytokine levels were determined with the Bio-Plex Human Inflammation Panel 1 37-plex assay kit (Bio-Rad, 171AL001M) according to the manufacturer’s instructions using the MAGPIX laboratory multianalyte profiling system (Millipore ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted 2-fold and 1 μL was used for each PCR reaction with the Biorad qRT PCR mix using a CFX Maestro (Biorad) machine ...
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bioRxiv - Immunology 2024Quote: ... 3.5 µL of 4x reducing buffer (1:10 ratio of beta-mercaptoethanol added to Laemmli buffer (Bio-Rad, catalog 1610747) was added to the diluted samples ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were stained with Ponceau S to visualize protein loading and VAChT was revealed with rabbit anti-VAChT (1:1000, SYSY, 139103) by ECL procedure (Clarity Western ECL, Biorad) on a chemiluminescence imager (ChemiDoc ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with secondary antibody diluted 1:7500 in blocking buffer (anti-rabbit, anti-mouse HRP, Bio-Rad). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher 32106) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
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bioRxiv - Bioengineering 2024Quote: ... and polystyrene disposable cuvettes (Semi-Micro, 1 cm path, 1.5 mL, 12.5 mm square x 45 mm high; Bio-Rad, 2239955). The alginate and CaSO4 were mixed ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were electrotransferred onto nitrocellulose membranes using the trans-blot turbo transfer system (constant voltage: 25V, maximum amperage: 1 A; 30 min, Biorad) and membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were used at 1:5000 dilutions and chemiluminescence signal was produced using the Clarity western ECL substrate (BioRad) and detected using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA samples were obtained from 1 µg RNA using the iScriptTM cDNA synthesis kit (Bio-Rad, Cat no. 170-8891) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
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bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of proteins at 10 µM mixed in a 3:1 ratio with 4X Laemmli Buffer Sample (Biorad) provided with 10% DTT ...
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bioRxiv - Immunology 2024Quote: ... The plates were then washed again and incubated for 30 minutes with 50 µl of either a 1:7000 dilution of goat IgG conjugated with horseradish peroxidase (HRP) having anti-chicken IgY specificity (Biorad), or 1:3000 dilution of goat IgG conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2024Quote: ... The HRP-coupled secondary antibodies were used at a 1:10,000 dilution followed by development using ECL substrate from Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... 20% SDS and transferred using Transfer Buffer (Running Buffer, 20% Methanol) for 1 h and 30’ on Nitrocellulose membrane (BioRad). Membranes were blocked in 5 % milk ((W/V ...
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bioRxiv - Pathology 2024Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011, Bio-Rad) in the blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Tween 20 and 0.5% (w/v) BSA with a secondary horseradish peroxidase-conjugated anti-rabbit antibody (1:6000, Bio-Rad). The chemiluminescence-based detection was performed by using the Clarity™ Western ECL substrate detection kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Boston, USA) diluted 1:10,000 (16, 17, 73) on a nitrocellulose membrane using the Bio-Dot Microfiltration kit (Bio-Rad). Bound antibodies were detected with a peroxidase-conjugated Goat anti-rabbit immunoglobulin G (IgG ...
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bioRxiv - Neuroscience 2024Quote: ... 2012) and cDNA was synthesized from 1 ug of RNA using the iScriptTM Reverse Transcription Supermix (Biorad, Hercules, CA, USA) thereafter ...
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bioRxiv - Systems Biology 2024Quote: ... the membranes were rinsed in TBS-Tween 20 buffer (TBST) and blocked for 1 hour in Every-Blot Blocking Buffer (BioRad) before being washed with TBST.
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bioRxiv - Molecular Biology 2024Quote: ... Fragmentation was stopped by addition of 80 μL 1 M Tris pH 6.8 and cleaned up twice with Micro Bio-Spin P-30 Gel Columns (BioRad, 7326223) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Polyacrylamide gels consisted of a resolving phase of 8-16% (w/v) acrylamide (37.5:1 acrylamide:bisacrylamide, 2.7% crosslinker; Bio-Rad, Watford, UK), 0.375 M Tris-HCl (pH 8.8) ...
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bioRxiv - Genomics 2024Quote: ... A total of 1 μg RNA was then used to synthesize cDNA through reverse transcription with the iScript cDNA synthesis Kit (BioRad). Quantitative gene expression analysis of Pax7 and the Pax7-Foxo1 fusion was performed using quantitative RT-PCR (qPCR) ...
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bioRxiv - Biophysics 2024Quote: ... and centrifuged (1 min, 230xg) prior to thermal denaturation using a CFX Connect Thermal Cycler (Bio-Rad, cat. no. 1855201). Fluorescence was measured using the HEX channel (excitation λ=515-535 nm ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescent 660 nm secondary Anti-mouse antibody (1:20.000) was incubated for 1 h at room temperature prior to fluorescence detection using a ChemiDoc Imaging System (Bio-Rad). Quantification of band densitometry was performed using the ImageLab software ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:20 and combined with primers for the target genes and SYBR Green master mix (Bio-Rad). Amplification was performed using standard conditions on a CFX96 Real-Time system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were generated by sonicating and boiling 1*10^6 cells in 100ul of 2X Laemmli Buffer (Bio-Rad) with BME ...
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bioRxiv - Cell Biology 2024Quote: ... and qRT-PCR was performed using PerfeCTa SYBR Green Supermix for iQ (QuantaBio) and primers (Table 1) in a iQ5 iCycler (BioRad). For detection of miR-126 and the control mRNA sno234 ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated overnight at 4°C with anti-F4/80 antibody (F4/80 antibody | Cl:A3-1, Bio-Rad, USA). Endogenous peroxidases were quenched by incubating the sections in 3% hydrogen peroxide (Acros Organics ...
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bioRxiv - Genetics 2024Quote: ... was added to the reactions and the products were separated on 6% polyacrylamide gels (ratio acrylamide:bisacrylamide 19:1, Bio-Rad) in TAE (40mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated for 1 h at room temperature with 0.1 μg/ml HRP- conjugated goat anti-rabbit (BioRad, 162-0177) in blotting buffer ...