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Citations for Bio-Rad :
3751 - 3800 of 9642 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by using Bradford assay (Bio-Rad). Tag-free Mical1 full-length bMERB domain ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to 0.2 μm nitrocellulose membranes (Bio-Rad), blocked for 1 hr at RT in 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad, 1704159) using the Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Relative protein levels were quantified using Image Lab (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was quantified using a Bradford assay (Bio-Rad). Samples were separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Protein quantitation was assessed by Bradford assay (Bio-Rad #5000006) using serial dilutions of bovine serum albumin (BSA ...
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bioRxiv - Synthetic Biology 2024Quote: ... along with a Precise Plus protein standard ladder (Bio-Rad). 14 μL of each cell lysate sample (1.3 μg of soluble protein ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were resolved by 4-15% gradient SDS gels (Biorad). The gels were dried and exposed to a phosphorimage screen for 24 hrs before being developed on the Typhoon FLA7000 (Cytiva).
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bioRxiv - Genomics 2024Quote: ... Protein concentration was determined using Bradford assay (Biorad 500-0006). 1-2 mg of protein extract was used per ChIP and 5% was taken out to use as input DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected using enhanced chemiluminescence substrate (107- 5061, BioRad). Images were acquired using detection systems Amersham ImageQuant 800 (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... or a semi-dry protein transfer system from BIO-RAD and analyzed by Western blotting ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Immunology 2020Quote: Supernatants were thawed and immediately analyzed with Bio-plex human cytokine assays (Bio-Rad Laboratories, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Immunology 2024Quote: ... 17 human cytokines were analysed simultaneously using the kit and run on the Luminex analyser (BIORAD), standard curves for each cytokine created and per cytokine concentrations calculated using the BioRad software.
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into K562s using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation for the protein lysate was performed by using SureBeads™ Protein G or A Magnetic Beads (purchased from Bio-Rad; catalog numbers1614023 and 1614013). Western blotting experiments were performed by using precast TGX 4-20% gels (purchased from Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... and then converted to units of fmol/mg of total protein added to an assay point (as measured by Bio-Rad DC Protein Assay Kit). Equilibrium dissociation constants (Kd ...
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bioRxiv - Neuroscience 2020Quote: ... Uptake was normalized to total mg of protein per sample measured via Bio-Rad DC protein assay kit (5000112 Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2019Quote: ... Protein content of muscle homogenate was measured in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA) against bovine serum albumin standards (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad, Hercules, CA, Catalog #5000006).
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations were analyzed using a Quick Start Bradford Protein Assay Kit with bovine serum albumin as a standard (Bio-Rad Laboratories, Hercules, CA, USA). The samples were standardized by diluting in the diluent ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting total protein was quantified using the Bio-Rad DC Protein Assay Kit according to the manufacturer’s recommended protocol (Bio-Rad, Cat#5000111, Hercules, CA, USA). Samples and standards were read on a BioTek Synergy HT plate reader after a 15 min incubation period ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Bioengineering 2021Quote: ... Proteins were separated and visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue R-250 (Bio-Rad, USA) staining ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: Concentrations of purified proteins were determined by the densitometry of bands on Coomassie Blue R-250 stained SDS-PAGE gels using carbonic anhydrase as a standard and a ChemiDoc MP imaging system (Bio-Rad Laboratories). Fluorescent proteins were detected by direct in-gel fluorescence imaging using the same ChemiDoc imaging system ...
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bioRxiv - Cell Biology 2019Quote: ... conjugates were analyzed by SDS-PAGE under reducing conditions on a 12% Tris-HCl polyacrylamide gel and stained with Coomassie Brilliant Blue R-250 (BioRad, Ontario, Canada). The migration distance in the gel relative to the blue dye front (Rf ...
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bioRxiv - Microbiology 2020Quote: ... Replicate gels were stained with Coomassie Blue R 250 or transblotted onto Immobilon polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA), reacted with affinity-purified YidC1 or YidC2 C-terminal-specific polyclonal rabbit antibodies (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: Protein concentrations were determined by the densitometry of bands on SDS-PAGE gels stained with Coomassie Blue R-250 using carbonic anhydrase as a standard and a ChemiDoc MP imaging system (Bio-Rad Laboratories). Fluorescent proteins were detected by direct in-gel fluorescence imaging using the same ChemiDoc imaging system ...
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bioRxiv - Zoology 2023Quote: ... quantitative R T–PCR was performed with a Bio-Rad CFX96™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) according to standard protocols and cycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Immunology 2021Quote: Proteins (20 µg per well) were separated in 4–15% Criterion™ TGX™ Precast Midi Protein Gels with 26 wells (Bio-Rad, Hercules, CA, USA) at a constant voltage of 120 V ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein estimations were performed using the Bio-Rad microtiter Bradford protein assay (California, USA) according to the instructions provided (Bio-Rad version: Lit 33 Rev C) and measurements were made at 595 nm using a SpectraMax Plus ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were adjusted to 5 µg of total soluble proteins in native sample buffer and then loaded onto a 4-20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, Cat. No. 4561094). Protein electrophoreses were performed in TG buffer (60 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Cell Biology 2019Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...