Labshake search
Citations for Bio-Rad :
3551 - 3600 of 4927 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... complementary DNA (cDNA) was synthesized from the RNA using the iScript cDNA Synthesis kit (BioRad, #170-8891). Quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Pathology 2024Quote: ... RNA extracts were treated with DNAse and reversed transcribed using gDNA Clear cDNA Synthesis kit (Bio-Rad). qPCR was conducted in 10 µl final volume with 2 μl of 5X HOT Pol EvaGreen qPCR mix plus (Euromedex) ...
-
bioRxiv - Pathology 2024Quote: ... RNA extracts were treated with DNAse and reversed transcribed using gDNA Clear cDNA Synthesis kit (Bio-Rad). qPCR was conducted in total reaction volume of 10 µl containing 2 μl of cDNA ...
-
bioRxiv - Genetics 2024Quote: ... 400 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad, 1708891). The cDNA region containing phased heterozygous SNP of ANXA11 – rs2573353 in WTC11141 – was amplified using the following primers ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were developed using the ECL kit and scanned with ChemiDoc MP Imaging system (Bio-Rad). The blots were quantified with Image Studio Lite software and GraphPad Prism were used for statistical analysis (unpaired t test ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1mM EDTA) and protein concentration was measured using the DC protein assay kit (Bio-Rad,5000112) and absorbance was read on the Synergy H1 ...
-
bioRxiv - Cell Biology 2024Quote: SDS-PAGE analysis was carried out either with 10% gels (TGX FastCast acrylamide Kit 10%; Bio-Rad) or precast 4-15% gradient gels (Mini-PROTEAN TGX precast gel ...
-
bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of total RNA were used for cDNA synthesis using an iScript cDNA Synthesis kit (Bio-Rad). Quantitative analysis of RPS6KA2 was performed using a SsoAdvanced™ Universal SYBR® Green Supermix (Biorad ...
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
-
bioRxiv - Cancer Biology 2021Quote: ... One microgram of RNA was reverse-transcribed to cDNA using the BioRad 5X iScript RT supermix kit (BioRad Laboratories Canada ...
-
bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with the Quick Start™ Bradford Protein Assay kit (Bio-Rad Laboratories, Hercules, CA) using a concentration series of bovine serum albumin solution as standards.
-
bioRxiv - Cell Biology 2019Quote: ... Cell lysates were cleared with centrifugation and protein concentrations were measured with Protein Assay kit (Bio-Rad, #5000001). 25-50 μg protein were loaded to 10-well SDS-PAGE gradient gel (Bio-Rad 456-1094) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... as well an Experion™ bioanalyser with a prokaryotic RNA standard sensitivity analysis kit (Bio-Rad, California, USA). One μg of RNA from each of the propleural plate samples was sent to Vertis Biotechnologie AG (Freising-Weihenstephan ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was collected and protein concentration quantified using a Bio-Rad protein assay kit (BioRad Laboratories, USA) and later stored at -80°C until use.
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then amplified by real-time quantitative polymerase-chain reaction (RT-qPCR, SybrGreen PCR MasterMix Kit, Bio-Rad) using primers listed in Table 1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the RNA was reverse transcribed to cDNA (Revertid First Strand cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) and then amplified by real-time quantitative polymerase-chain reaction (RT-qPCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared with 1000ng of DnaseI treated RNA (iScript Select cDNA synthesis Kit, BioRad, following manufacturer’s instructions). Random hexamers were used for the nascent RNA experiment showed in Figure 2F and Figure 2 - figure supplement 2A ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was used to make cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). All primer pairs were designed and validated in-house for efficiency and specificity ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix kit (Biorad, 1708840). Quantitative RT-PCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
-
bioRxiv - Molecular Biology 2020Quote: ... Pd-PR and Ac-PR were detected using antiFlag antibody with clarity western ECL substrate kit (Bio-Rad).
-
bioRxiv - Molecular Biology 2021Quote: cDNA was reverse transcribed from 10 ng total RNA using an iScript Advanced cDNA synthesis kit (Bio-Rad). ddPCR was performed by using ddPCR EvaGreen Supermix kit (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... 400-500 ng RNA was used in 20μl cDNA synthesis reactions using iScript cDNA Synthesis Kit (Bio-Rad). Three biological replicates were used for the PCR analysis or sequencing.
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from 1000 ng of RNA using the iScript cDNA synthesis kit (Bio-Rad cat #1708891) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... was used to isolate total RNA and mRNA was reverse transcribed into cDNA using iSuperscript kit (Bio-Rad). QPCR was performed using SYBR Green kit (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... cDNA was generated from up to 1 μg of RNA with the iScript cDNA Synthesis kit (Bio-Rad). Quantitative PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... melanogaster y1w1118 adult flies subjected to the monoterpene exposures using Aurum Total RNA Mini Kit (Bio-Rad, USA). One µg of RNA was treated with DNase I (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 µL input and the entire IP fractions were reverse transcribed using the iScript cDNA synthesis kit (BioRad) and subjected to RT-qPCR ...
-
bioRxiv - Microbiology 2019Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
-
bioRxiv - Cancer Biology 2019Quote: ... Membrane fractions were dissolved in MES buffer and protein concentration was determined by the DC assay kit (Biorad). For γ-secretase assays ...
-
bioRxiv - Physiology 2020Quote: ... and 1000 ng of RNA was used for cDNA synthesis using iscript cDNA kit (Bio-Rad Laboratories Inc.). Quantitative real-time PCR was performed using the Applied Biosystems 7500 Real-Time PCR System ...
-
bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA was synthesized by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Samples were quantified and amplified using SensiMix (Bioline ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™ ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 μL of the BALF sample was used with BioRad 23-plex-Group I kit (BioRad Cat# M60009RDPD) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Complement DNA (cDNA) was transcribed from 300-400 ng of RNA with the iScript cDNA Synthesis kit (BioRad) and the resulting cDNA was mixed with SYBR Green Master Mix (ABI ...
-
bioRxiv - Molecular Biology 2019Quote: ... One microgram of total RNA from each exosome sample was converted to cDNA (Supermix iScript kit, Bio-Rad). A one in 10 dilution of cDNA was used for qPCR reaction mixture with final concentration of 1× SsoAdvanced universal SYBR super mix (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: ... The quality of RNA was then assessed using the Experion RNA StdSens analysis kit (Bio-Rad, Hercules, CA). Based on the RNA-quality index (RQI) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was prepared from 1 μg of RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). cDNA samples were diluted 1:10 and real-time PCR was performed using IQ SYBR Green supermix and gene-specific primers in the iCycler real-time PCR detection system (Bio-Rad) ...
-
bioRxiv - Bioengineering 2022Quote: ... The transformation was performed using the protocols from the pGLO Bacterial Transformation Kit (Bio-Rad Laboratories, Hercules, CA). In each experiment ...
-
bioRxiv - Bioengineering 2020Quote: ... we first used a High Capacity cDNA Reverse Transcription Kit on a MyCycler (Bio-Rad, Hampton, NH, USA). Then ...
-
bioRxiv - Bioengineering 2021Quote: ... First strand cDNA was synthesized utilizing 2 µg of total RNA and iScriptTM cDNA Synthesis Kit (Bio-Rad). A fraction (0.75 μg ...
-
bioRxiv - Bioengineering 2020Quote: Equal amount of total RNA was converted to cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, USA) using manufacturer’s guidelines ...
-
bioRxiv - Biochemistry 2021Quote: ... 1μg of total RNA was used for cDNA synthesis using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Quantitative real-time RT-PCR was performed on the ABI Prism 7900 Sequence Detection System (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2021Quote: ... Alkaline phosphatase activity was detected with BCIP/NBT AP-conjugate substrate reaction kit (Bio-Rad, Hercules, CA, USA) after post-secondary washes with TBS-T ...
-
bioRxiv - Neuroscience 2020Quote: ... Total RNA was quantified using NanoDrop1000 and was converted to cDNA using iScript cDNA synthesis kit (Bio-Rad) on a SimpliAmp Thermal cycler (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA synthesis kit (Bio-Rad). A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche) ...
-
Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... Western blot bands were visualized by chemiluminescence using the Clarity Max Western blotting kit (Bio-Rad Laboratories, #1705062) and the ChemiDoc imaging system ...
-
bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was obtained by retrotranscription of 1 μg of total mRNA with iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...