Labshake search
Citations for Bio-Rad :
3551 - 3600 of 3951 citations for 6 amino m xylene 4 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract was loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Biorad). After transfer onto nitrocellulose membrane ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were electrophoretically resolved on gelatin-containing gels at 125 V for 110 min at 4 °C using a Power Pack supply (Bio-Rad, USA) Next ...
-
bioRxiv - Microbiology 2020Quote: ... The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn). Non-His-Tagged proteins were eluted with lysis buffer until the absorbance at 280 nm matched background levels utilizing 50 – 100 mL of lysis buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
-
bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
-
bioRxiv - Bioengineering 2021Quote: ... 10 µg/lane of S was run on 4–20% Mini- PROTEAN® TGX Stain-Free™ Protein Gels (BioRad, Cat#4568081), transferred to nitrocellulose (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples with equal protein (20ug) content were combined with loading buffer and separated using standard gel electrophoresis on 4-20% polyacryllamide gel (Bio-Rad Techonolgies) and then partial-wet transferred to nitrocellulose membranes (BioRad) ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were then transferred directly to ice-cold A4P0 solution consisting of: 4% acrylamide monomer (40% acrylamide solution, cat. 161-0140, Bio-Rad, UK), 0.25% VA-044 (thermoinitiator ...
-
bioRxiv - Cancer Biology 2022Quote: ... Equal amount of protein from different samples and 5-μl of Odyssey One-Color Protein Molecular Weight Marker (LiCore Cat# 928-40000) were resolved on 4—15% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE, Biorad Cat#4561086) then the protein was transferred to a nitrocellulose membrane at 4 °C overnight at 30 volts ...
-
bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
-
bioRxiv - Plant Biology 2019Quote: ... Ten microliters of the cell extract was subjected to 4-20% sodium dodecyl sulfate polyacrylamide electrophoresis gels (SDS-PAGE, Bio-Rad, CA). SDS-PAGE containing proteins were transferred onto PVDF membranes (Bio-Rad ...
-
bioRxiv - Biophysics 2019Quote: ... Fractions were analyzed after separation in stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad). These stain-free gels contain trihalo compounds that undergo UV-induced covalent modification of tryptophan residues for subsequent fluorescent detection ...
-
bioRxiv - Biophysics 2019Quote: ... The purified protein samples obtained after the final SEC step were analyzed by stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad).
-
bioRxiv - Cancer Biology 2019Quote: ... SDS-PAGE was carried out by using 4-20% SDS CriterionTM TGXTM pre-cast gels in a cell separation unit Criterion DodecaTM (BIO-RAD, Australia), in the buffer containing Tris-glycine-HCl at 200 V for 100 min ...
-
bioRxiv - Biophysics 2019Quote: ... was utilized to monitor the elution of the proteins and fractions were analyzed after separation on 4– 15% precast gradient TGX Stain-FreeTM gels (Bio-Rad Laboratories).
-
bioRxiv - Developmental Biology 2019Quote: ... and 1X phosphatase inhibitor cocktail and equal amounts of protein samples were loaded and run in a 4 % to 20 % SDS gel (Bio-Rad Laboratories). Protein was transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...
-
bioRxiv - Plant Biology 2020Quote: Sample loading buffer was added and equivalent amounts of protein (~ 30 μg) separated by SDS-PAGE (1.0 mm thick 4 to 12% polyacrylamide Criterion Bis-Tris XT-gels, Bio-Rad or equivalent) in MOPS buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... Final extension was at 72°C for 7min and the reaction was stopped by holding tubes at 4°C until removed from the thermal cycler (MyCycler™ Thermal Cycler BIORAD, USA). The PCR products were electrophoresed in a 1.5% agarose gel stained with SYBR Safe DNA Gel stain (Invitrogen ...
-
bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
-
bioRxiv - Neuroscience 2019Quote: Protein extracts were loaded at 20μg per lane and resolved by standard electrophoresis in 4-20% Mini-PROTEAN TGX Precast polyacrylamide protein gels (Bio-Rad, Hercules, CA). Gels were transferred onto polyvinyl difluoride membranes (PVDF ...
-
Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... using 7.5 % polyacrylamide gels for Rad53 detection or a gradient of 4-15 % polyacrylamide for all other proteins in Mini-PROTEAN® electrophoresis chambers (Bio-Rad). Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Immunology 2019Quote: ... The protein pellet was washed twice with methanol before boiling in 1X non-reducing loading buffer for 5 min and loading onto a 4-15% TGX stain-free gel (Bio-Rad 4568085). Following SDS-PAGE ...
-
bioRxiv - Bioengineering 2020Quote: Mouse tissues from mdx4cv mice treated with or without AAV9-NG or AAV9-iNG were lysed with cold RIPA buffer supplemented with protease inhibitors and extracted protein samples were separated by SDS-PAGE (BioRad, 4-15%) and transferred onto Nitrocelluloase membranes (0.45 μm) ...
-
bioRxiv - Immunology 2021Quote: ... The samples were mixed by pipetting and loaded at 30 μg to a 4-20% 10-well Mini-PROTEAN TGX™ precast gel (Bio-Rad). Ten microliters of the Novex™ Sharp Pre-stained Protein standard (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and 8 μg of protein was added from each sample to a 26-well Criterion TGX 4-20% precast gel (Bio-rad, 5671095). Proteins were transferred to an Immun-Blot low flouresence PVDF membrane (Bio-rad ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 20 µg of protein from each sample was separated by SDS-PAGE on 4–20% precast polyacrylamide gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using the following antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
-
bioRxiv - Cell Biology 2021Quote: ... The cell lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084) and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
-
bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
-
bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
-
bioRxiv - Molecular Biology 2021Quote: ... even protein amounts were loaded and separated on mini-PROTEAN TGX precast 4-15% Bis-Tris gels (#4568085, Bio-Rad, Copenhagen, Denmark). Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159 ...
-
bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
-
bioRxiv - Immunology 2021Quote: ... Parasite lysate was reduced with β-mercaptoethanol (BME) and separated via SDS-PAGE in 4-20% Mini-PROTEAN TGX pre-cast gels (cat # 4561096, Bio-Rad) before transfer to PVDF membrane using a Trans-Blot Turbo Mini PVDF Transfer Pack (cat # 1704156 ...
-
bioRxiv - Cell Biology 2019Quote: ... The reaction was quenched by incubation with 1 mM TCEP for 20 minutes at 37°C and 60% of the eluate was run on an SDS-PAGE gel (Mini-Protean TGX, 4-15%, Bio-Rad #4561084). The gel was stained with colloidal Coomassie (Bio-Rad #1610803 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The thawed samples were separated by SDS-PAGE using Mini-PROTEAN® TGX Stain-Free™ gels (usually 4-20%, Bio-Rad), and subsequently transferred to nitrocellulose membranes by a semi-dry blotting procedure as per manufacturer guidelines ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
-
bioRxiv - Cancer Biology 2022Quote: ... An equal amount of protein from control and each experiment was loaded onto 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Gels, Bio-Rad, USA) and transferred to the nitrocellulose membranes (Amersham™ Protran® 0.45 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples and PageRulerTM Prestained Protein Ladder (ThermoScientific) were loaded onto Mini-PROTEAN® precast gradient (4-20 %) polyacrylamide gels (Bio-Rad Laboratories) and proteins were separated in an electric field of 90-120 V for varying time spans in SDS Running Buffer (25 mM Tris-base ...
-
bioRxiv - Immunology 2022Quote: ... and equal amounts of protein were loaded into a 4-15% gradient polyacrylamide gel (BioRad TGX™ Precast Midi Protein Gel #5671085) and proteins separated based on molecular weight using electrophoresis at 110V ...
-
bioRxiv - Molecular Biology 2022Quote: ... 30 μg protein of whole cell lysate or variable volume of Flag-IP samples was loaded into 4-20% Mini-PROTEAN® TGX Stain-Free(tm) Gels (Bio-Rad) and then transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Microbiology 2022Quote: ... Equal volumes of sample were separated on 4–12% or 10% Criterion XT Bis-Tris Precast gels (Bio-Rad, Hercules, CA, USA) using XT MES Running buffer (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
-
bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein extract (20–30 μg) were resolved by SDS-PAGE (4–15% gradient precast TGX polyacrylamide gel, Bio-Rad Laboratories), followed by standard western blot procedure ...