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Citations for Bio-Rad :
3501 - 3550 of 3629 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad] ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then incubated with respective primary and secondary antibodies before image acquisition using the ChemiDoc MP imaging system (Bio-Rad). Densitometry analysis was conducted using ImageJ and protein expression levels were normalized to α-tubulin loading control.
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bioRxiv - Neuroscience 2024Quote: ... The following day after washes with TBS-T and incubation with secondary antibodies (Goat Anti-Rabbit or Goat Anti-Mouse IgG (BioRad 1706516) 1:5000 dilution in 10% milk ...
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bioRxiv - Microbiology 2024Quote: ... for 1 hour RT followed by 1:2000 IRDye800 conjugated goat-anti-rabbit antibody (Li-COR Biosciences) or 1:1000 goat-anti-rabbit HRPO (Bio-Rad) for 1 hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 60 μL of secondary goat antibody anti-rabbit IgG coupled with horseradish peroxidase (Bio-rad Goat Anti-Rabbit IgG (H+L) HRP conjugate 170-6515 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Neuroscience 2021Quote: Western blotting experiments were performed on hippocampal tissue and specific bands were visualized with secondary HRP-conjugated antibodies using ChemiDoc™ Imaging Systems (Bio-Rad). The relative intensities of immunoblot bands were determined by densitometry with ImageJ software ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The presence of the secondary antibody was confirmed using the Pierce™ECL Plus Western blotting substrate (ThermoScientific, USA) and viewed using the ChemiDoc (BioRad, USA).
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Blotted membranes were washed three times with 0.05% Tween 20 in Tris-HCl buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against rabbit IgG (Bio-Rad Laboratories) at 1:10,000 dilution in Can Get Signal Solution 2 (TOYOBO ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Each PVDF-membrane piece (per antibody) was separately imaged via the auto-exposure function of the ChemiDoc-MP imaging system (Bio-Rad Laboratories). Supplementary-Fig.S4B legend describes the WB-signal quantification in detail ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was first probed with the indicated primary antibodies and then incubated with goat anti-rabbit (Bio-Rad, cat. no. 1706515) or goat anti-mouse (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for Western blotting were: Donkey anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA). Secondary antibodies used for immunofluorescence were ...
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bioRxiv - Cancer Biology 2020Quote: ... Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no. 1705061, Bio-Rad, Gladesville ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Microbiology 2021Quote: ... The purity of the eluted fractions was assessed by SDS-PAGE and Western blot using a mouse anti-histidine antibody (BioRad, AbD Serotec).
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Antibody binding in immunoblot analyses was detected using horse radish peroxidase-conjugated secondary antibodies and visualized using enhanced chemiluminescent chemistry (BioRad Clarity™) and visualized via Bio-Rad Chemidoc ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody, clone SV5-Pk1, Bio-Rad). The unbound fraction was discarded and the beads washed 2 times with 1ml of IP Buffer and additionally twice more with 1 ml ice cold PBS + protease inhibitors ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Blots were incubated with α-TPLATE appendage antibodies (rabbit) (Dejonghe et al, 2019) and imaged on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... Complete antibody conjugation was validated by running both conjugated and un-conjugated paired antibodies on a 4-15% SDS-PAGE gel (Bio-Rad #4561083) under non-reducing conditions ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (Cytiva) and images were acquired on ChemiDoc Touch (Bio-Rad Laboratories). The acquired images were processed and quantified using Image Lab software (Bio-Rad laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).