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Citations for Bio-Rad :
3451 - 3500 of 3625 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary HRP-linked antibody was incubated for 1 hour at room temperature and developed using Clarity Western ECL blotting substrate (BioRad, 1705061).
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... The membranes were subsequently probed with a primary antibody against β-ACTIN as a loading control or stained with Bio-Safe Coomassie stain (Biorad), dried ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP and the Clarity Western enhanced-chemiluminescence (ECL) substrate as describes by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant Las 4000 luminescent image analyzer.
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies associated signal was detected with ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the corresponding signal obtained was done using Image lab software.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Neuroscience 2023Quote: ... The antigen–antibody complexes were detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) using the ChemiDoc XRS+ Imager System (Bio-Rad). Blots were exposed for 3 seconds ...
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bioRxiv - Pathology 2023Quote: ... HRP- conjugated secondary antibody and ECL chemical luminescence reagents were used to detect immunoblot signal in the Gel-doc system (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with HRP-linked secondary antibodies and developed with Clarity or ClarityMax chemiluminescent substrates using a ChemiDoc XRS+ imager (Bio-Rad). (See Supplementary Methods Table 3 for antibody information).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then incubated with respective primary and secondary antibodies before image acquisition using the ChemiDoc MP imaging system (Bio-Rad). Densitometry analysis was conducted using ImageJ and protein expression levels were normalized to α-tubulin loading control.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... CD206 was detected using rat anti-mouse CD206 (dilution 1/150, Bio-Rad, cat. MCA2235GA) and Alexa Fluor® 546 goat anti-rat antibody (dilution 1/250 ...
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bioRxiv - Molecular Biology 2020Quote: ... and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad, Cat NO.: 170-6516), respectively ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently with Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad, Cat. 1706516). The blot was developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The slides were air dried and incubated with anti-rabies nucleocapsid conjugate (Bio-Rad, France) for 35 min at 37°C in a humid chamber and further washed with phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Acid-treated fiber spreads were incubated with 1/1000 rat anti-BrdU mAb (Bio-Rad) for 1.5 hour to detect CldU ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... 50 μl of horseradish peroxidase-conjugated goat anti-pig IgG (diluted 1:10000, Bio-Rad) or anti-pig IgA (diluted 1:10000 ...
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bioRxiv - Bioengineering 2019Quote: ... A 1:5000 dilution of the “Immun-Star Goat Anti-mouse-HRP conjugate” (1705047, Biorad) was used as secondary antibody under the same conditions ...
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bioRxiv - Cell Biology 2021Quote: ... Goat anti-mouse IgG (H/L):HRP (1:1,000; multi species absorbed; Bio-Rad, STAR117P) and ECL western blot detection reagents (Cytiva ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was incubated with anti-GFP (10 μg) immobilized on UNOsphere SUPrA beads (Biorad) at 4 °C for 2.5 hr ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse IgG conjugated to horseradish peroxidase (HRP; Bio-Rad, Germany cat no. 1706516). Absorbance was measured at an optical density of 450 nm with a Multiskan FC plate reader (Waltham ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Bio-Rad; 1:2000).
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary anti-mouse-Starbright Blue-520 (Bio-Rad# 64456855; 1:2000) and anti-Rabbit-Starbright Blue-700 (Bio-Rad# 64484700 ...
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bioRxiv - Physiology 2024Quote: ... NE) and Starbright Blue 700 goat anti-mouse at 1:5,000 #12004159 from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Microbiology 2024Quote: ... or with 0.1 µg/well of mouse anti-human IgG (CH2 domain) clone R10Z8E9 (BioRad) for Fc capture in carbonate buffer ...
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bioRxiv - Microbiology 2024Quote: ... Western blots were revealed using Goat anti-mouse IgG (H+L) HRP conjugate (Bio-Rad) and membranes were washed between the different incubations for 2 cycles of 10 minutes with TBS-T ...
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bioRxiv - Neuroscience 2021Quote: Western blotting experiments were performed on hippocampal tissue and specific bands were visualized with secondary HRP-conjugated antibodies using ChemiDoc™ Imaging Systems (Bio-Rad). The relative intensities of immunoblot bands were determined by densitometry with ImageJ software ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Biochemistry 2020Quote: ... The presence of the secondary antibody was confirmed using the Pierce™ECL Plus Western blotting substrate (ThermoScientific, USA) and viewed using the ChemiDoc (BioRad, USA).
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Each PVDF-membrane piece (per antibody) was separately imaged via the auto-exposure function of the ChemiDoc-MP imaging system (Bio-Rad Laboratories). Supplementary-Fig.S4B legend describes the WB-signal quantification in detail ...
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bioRxiv - Cancer Biology 2020Quote: ... Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no. 1705061, Bio-Rad, Gladesville ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Antibody binding in immunoblot analyses was detected using horse radish peroxidase-conjugated secondary antibodies and visualized using enhanced chemiluminescent chemistry (BioRad Clarity™) and visualized via Bio-Rad Chemidoc ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete antibody conjugation was validated by running both conjugated and un-conjugated paired antibodies on a 4-15% SDS-PAGE gel (Bio-Rad #4561083) under non-reducing conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (Cytiva) and images were acquired on ChemiDoc Touch (Bio-Rad Laboratories). The acquired images were processed and quantified using Image Lab software (Bio-Rad laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).