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Citations for Bio-Rad :
301 - 350 of 4333 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the samples were gradually heated from 4 to 98°C with a rate of 0.2°C/10 s in the CFX96 Real-Time System C1000 Touch Thermal Cycler (Biorad). The fluorescence intensity was measured using an excitation wavelength of 470 nm while monitoring emission at 570 nm.
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bioRxiv - Neuroscience 2022Quote: ... Chemiluminescence and Ponceaus S signal density were each imaged using a ChemiDoc XRS imaging system (170-8070, Bio-Rad) and quantified using Image Lab software (RRID:SCR_014210) ...
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bioRxiv - Physiology 2022Quote: ... S-nitrosylated proteins were separated using 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). A monoclonal anti-Cx43 antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... IgM-containing Capto L fractions were pooled and subjected to cation exchange chromatography using Nuvia HR-S (Bio-Rad) with an equilibrating and wash buffer (20 mM acetate ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence was measured at 512 nm along a temperature gradient from 20 to 100 °C (0.5°C increase every 10 s) in the CFX ConnectTM real-time thermocycler (BioRad) with the Cfx MaestroTM software ...
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bioRxiv - Neuroscience 2023Quote: ... and the transgene sequences were amplified with an Eppendorf Mastercycler pro S using iTaq DNA polymerase (Bio-Rad #1708870), a dNTP mix (Bio-Rad #1708874) ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added to stop the reaction before performing the reading at 450 nm in the ELISA plate reader (iMark Microplate Reader, Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Germany) and lysosomes with rat monoclonal anti-Cd68 (clone FA-11, #MCA1957, Bio-Rad, Hercules, CA, USA). In the second set ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Neuroscience 2022Quote: ... was used for quantitative real time PCR with commercially available rat Magel2 and Gapdh primers (Bio-Rad, USA ...
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bioRxiv - Neuroscience 2024Quote: ... For analysis the Bio-Flex Pro™ Rat Cytokines Assay (Bio-Rad, 24-plex panel #171-K1001M) was used on a Bio-Plex® MAGPRIX™ multiplex reader and analysed with Bio-Plex Analysis software (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... Then membrane was then placed inside a transparency and exposed 10-30 s on a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR (Bio-Rad). Melting temperatures were calculated with DSFWorld61 using model 1 in the “by sigmoid fitting” option ...
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bioRxiv - Microbiology 2022Quote: ... the plates were sterilized using the sterilization setting (90 s) in a GS Gene Linker UV Chamber (Bio-Rad, Inc.). 500 µl of overnight cultures of S ...
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Microbiology 2019Quote: ... according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad). cas9 mRNA and 16srRNA were analyzed with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... The S and P samples were solubilized by addition of an equal volume of 2X Laemmli SDS-PAGE sample buffer (Biorad) and boiling for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... We utilized colorimetric detection to visualize the MBP-Vpu band(s) on the membrane after WB transfer: Primary mouse anti-histidine tag antibody (BioRad) and secondary goat anti-mouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were stained with Ponceau S and total proteins were visualized using the ChemiDoc™ MP imaging system (Bio-Rad). To detect proteins of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Plant Biology 2023Quote: ... The temperature ramp rate was 1°C/20 s and protein unfolding was monitored by the increase in RFUs produced every 10 s in a CFX Opus 96 System (BioRad). The average trace of 6 replications (2 at each concentration ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were pelleted by centrifugation at 500 rpm for 30 s and resuspended in 4x laemmli-sample buffer (Bio-Rad). Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: Target transcripts were amplified using the primers listed below (Integrated DNA Technologies, Inc.) and Sso Advanced Universal SYBR Green Supermix according to the manufacturer′s protocol (BioRad). All qPCR reactions were assayed in triplicate for each sample ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR with CFX Manager software (Bio-Rad). Melting temperatures were calculated with DSFWorld27 (by model 2).
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (BioRad). Results were analyzed by the Comparative Ct Method (ΔΔCt Method ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 35 °C to 79°C at a rate of 0.3 °C per 15 s in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Fluorescence intensity was determined using excitation (λem=525/20 nm ...
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bioRxiv - Zoology 2021Quote: The quantitative determination of IFN-ɣ in buffalo serum was assayed by a commercial bovine IFN gamma sandwich ELISA test (Bio-Rad) following the manufacture’s specifications ...
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bioRxiv - Immunology 2020Quote: ... RBD-specific antibody titres in oral and nasal swab fluids were determined by ELISA as detailed above except that the conjugated secondary antibody was replaced with either goat anti-porcine IgG HRP (Bio-Rad Antibodies) at 1:20,000 dilution in PBS with 1% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were stained with rat monoclonal anti-BrdU [clone BU1/75 (ICR1)] (BioRad Cat #MCA 2060, RRID: AB_323427), rabbit monoclonal anti-active Caspase-3 (clone C92-605 ...
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bioRxiv - Immunology 2019Quote: ... Rosa26LSL-tdTomato/+ animals (6-12 weeks) were given 1 μg of FITC-conjugated rat anti-CD169 antibody (BioRad) diluted in a total volume of 20 μl of PBS into the right footpad to label CD169+ subscapular macrophages inside the draining LN ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-rat (1:1000; Alexa Fluor 568, #A11077) and V5-tag:AlexaFluor-647 (1:200; Bio-Rad MCA1360A647).
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bioRxiv - Cancer Biology 2020Quote: 10 μm sections of mouse tumors were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Physiology 2021Quote: Quantitative PCR for rat FXR and reference gene GAPDH was performed using SsoAdvanced Unviersal SYBR Green Supermix (BioRad) in the ViiA 7 Real-Team PCR System (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: ... incubated with primary antibody (monoclonal rat anti-F4/80, 1:200 dilution, MCA497; BIO-RAD; Hercules, CA, USA) for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.2–2.2 Mb, S. cerevisiae Ladder: Bio-Rad, 1703605; CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder: Bio-Rad, 1703667) and PFGE was performed using the CHEF Mapper XA System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleavage-related change in fluorescence signal of the probe was measured every 30 s for 60 min under incubation at 37°C using a Real-time PCR system (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Plant Biology 2019Quote: ... subsequently the powder was dissolved and desalted as above then incubated with Macro-Prep High S cation exchange media (Bio-Rad). The supernatant and five water washes were again collected and stored ...
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bioRxiv - Microbiology 2021Quote: ... according to the respective manufacturer’s protocols with an annealing temperature of 58 °C during real-time PCR in a CFX96 real-time system (Bio-Rad) thermocycler.
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bioRxiv - Immunology 2020Quote: ... Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and electrophoresis in Tris-glycine buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Microbiology 2022Quote: ... the displayed S-trimer protein bands on SDS-PAGE gels were scanned and quantified by ChemiDoc MP imaging system (Bio-Rad) and ImageJ.