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Citations for Bio-Rad :
301 - 350 of 371 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were introduced into recipient strains using a standard electroporation protocol according to the manufacturer’s instructions (BioRad Gene Pulser Xcell ...
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bioRxiv - Biochemistry 2021Quote: ... Guide RNA plasmids were transformed into electrocompetent cells containing the Cas protein using a MicroPulser Electroporator (Bio-Rad). Cells were recovered for 100 min at 37°C in LB broth ...
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bioRxiv - Microbiology 2020Quote: ... 100μl of electrocompetent cells was mixed with 10μl (500ng-1μg) of plasmid DNA and transferred to 2mm ice-cold electroporation cuvettes (Gene Pulser Cuvette (BioRad) and tapped gently to avoid air bubbles ...
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bioRxiv - Immunology 2022Quote: ... 25 µg (1 µg/µL concentration) of plasmid DNA was added to electroporation 4 mm cuvettes (Bio-Rad) first and then cells added to the cuvettes ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting plasmids were introduced into each WT cell using particle bombardment (PDS-1000/He, BioRad, Tokyo, Japan), and transformants expressing GFP were screened by fluorescence microscopy (Tachibana et al. ...
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bioRxiv - Microbiology 2024Quote: ... The cell/plasmid mixture was transferred to a chilled Gene Pulser 0.1 cm Cuvette (Bio-Rad 165-2089) and electroporated in a Gene Pulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... and transformed with 300-500 ng of methylated plasmid DNA using a Gene Pulser Xcell (Bio-Rad, Germany) at 2000 V ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Young leaves of aseptically grown tobacco plants were bombarded with plasmid-DNA-coated 0.6 μm gold particles (Bio-Rad) and selected on RMOP media with 500 μg ml-1 spectinomycin (Svab and Maliga 1993) ...
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bioRxiv - Neuroscience 2022Quote: ... At 8DIV cultures were shot with 1.6 micron Gold micro-carriers coated with 30 μg of hSyn.iGABASnFR.F102G plasmid using the Helios gene-gun system (Bio-Rad). Following transfection cultures remained for 5-10 days before experiments were carried out.
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bioRxiv - Synthetic Biology 2022Quote: ... 50 μL of Bt resuspension was electroporated in a 0.1 cm cuvette at RT with 0.5 μL of pDonor plasmid (average ~200 ng) at 2.2 kV using a MicroPulser (Bio-Rad). The cells were recovered in 1 mL of SOB at 37 °C for 2 hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were transfected into PEFs by electrical transfection in the condition of 170V/15ms (BIO-RAD, Gene Pulser Xcell). Selection culture medium supplemented with 400 μg/ml G418 for random integration cells and 400 μg/ml G418 ...
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bioRxiv - Immunology 2021Quote: ... Cells and plasmid DNA in solution were transferred into 0.2 cm generic electroporation cuvettes (Biorad Gene Pulser; Bio-Rad Laboratories ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation with 100 ng of the temperature sensitive plasmid pCas (0.2 cm-gap cuvettes (Bio-Rad), in a Gene pulser Xcell electroporation system (Bio-Rad)) ...
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bioRxiv - Systems Biology 2023Quote: ... the culture was harvested for electroporation with the pooled pTS041 plasmid library (0.1 cm Gene Pulser Cuvette #165-2089 and Micropulser, BioRad). Cells were recovered in SOC medium with kanamycin and 1 µM aTc for Cas9 induction at 30°C for 2 h and streaked out on to LB agar plates with kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... The resulting donor DNA was electroporated in the plasmid-bearing host using Gene Pulser Xcell Electroporation Systems (Bio-Rad) and the successfully edited clones were selected via colony PCR and Sanger sequencing of the motAB locus ...
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bioRxiv - Microbiology 2023Quote: ... A total of 50 μL of cells was mixed with plasmids and then subject to electroporation (Bio-Rad, USA) at 2.5 kV ...
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bioRxiv - Microbiology 2023Quote: ... We added 1.0 mg of various CRISPRi plasmids to the 0.2 ml fusobacterial competent cells in a chilled cuvette (0.1 cm electrode gap, Bio-Rad), allowing it to rest on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA and siRNA oligonucleotides were transfected into U373 cells using electroporation with a GenePulser Xcell (Bio-Rad Laboratories). Electroporation was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using 20 mg of pGP-pcDNA3.1 Puro-CAG-Voltron2-ST plasmid DNA and 1.6 μm gold microcarriers (Bio-Rad). Slices were transfected using a 3D-printed version of the modified barrel design (O’brien et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pML104-GAL1 and repair DNA were co-transformed into yeast by electroporation under Gene Pulser Xcell (Bio-Rad) conditions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Molecular Biology 2020Quote: ... ½ of a confluent T75 flask of WT F1 2-1 MS2129 female ESCs were electroporated with 40μg of PciI linearized PCR2.1-Puro XistΔE targeting plasmid (800v, 0.2ms, 4mm cuvette, Biorad X-Cell electroporation module) and plated at varying dilutions on 10cm plates of confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2020Quote: ... were electroporated with 40μg of Not1 linearized paa2Δ1.7 plasmid DNA (800v, 0.2ms, 4mm cuvette using a Biorad X-Cell electroporation machine) and plated on confluent irradiated DR4 feeders ...
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bioRxiv - Microbiology 2020Quote: ... at 5% parasitemia was resuspended in 300 μL CytoMix containing 75 μg plasmid DNA in a 0.2-cm electroporation cuvette (Bio-Rad). Electroporation was performed at 0.31 kV and 950 μF with maximal capacitance using a Gene Pulser II system (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cell/plasmid mixture was transferred to an electroporation cuvette with 1mm gap and transformed using a Gene Pulser Xcell electroporator (BioRad) with 1.8 kV ...
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bioRxiv - Biochemistry 2019Quote: 40 µl of electrocompetent cells were transformed with 1 ng of the lesion-containing or lesion-free control plasmids together with 1 ng of pVP146 (transformation control) by electroporation (GenePulser Xcell(tm) from BioRad, 2.5kV – 25µF – 200Ω) ...
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bioRxiv - Developmental Biology 2019Quote: ... ~250,000 trypsinised hESCs were resuspended in 600 μl PBS+Ca2++Mg2+ containing 50 μg gRNA plasmid and 50 μg homologous repair donor plasmid and electroporated in a 0.4-cm cuvette using Gene Pulser Xcell System (Bio-Rad) with a single 20 ms square-wave pulse (250 V) ...
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bioRxiv - Synthetic Biology 2020Quote: ... putida cells with selected plasmids using a MicroPulser electroporator and Gene Pulser Cuvettes with 0.2 cm gap (Bio-Rad, USA). Preparation of P ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad). After transfection ...
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bioRxiv - Plant Biology 2023Quote: ... 1×108 cells were plated on an ESAW agar plate and five μg of plasmid DNA coated on W-microparticles (M17, Biorad) was delivered into the cells using the Biorad Biolistic Particle Delivery System (1550 psi ...
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bioRxiv - Cell Biology 2023Quote: ... Episome plasmids were transformed into E. coli (TransforMax EPI300) harboring the pTA_Mob51 mobility plasmid (gift from R. Lale) via electroporation (Bio-Rad). Transformed cells were spread onto LB agar plates containing both gentamycin (10 μg mL-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The working plasmids were added to 80 µL competent cells in a pre-cooled 0.1-cm MicroPulser Electroporation Cuvette (Bio-Rad) and the mixture was treated in MicroPulser Electroporator (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... amoebiformis CCMP2058 cells were transformed with 10 μg of the plasmid using a Gene Pulser Xcell electroporation system (Bio-Rad), as described previously (Fukuda et al ...
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bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.0 millions of stably transduced cells were electroporated with 50 μg plasmids by the Gene Pulser X cellTM Electroporation System (Bio-Rad) at 250 V and 1500 microfarads ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-10 pg of pUC18 plasmid were electroporated at 18,000V/cm at a time constant of 7.5 msec using Gene Pulser Xcell (Bio-Rad, Hercules, CA) into 10 μl competent cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli cells were prepared using 10% glycerol (Applichem) and transformed with the corresponding plasmids using a “MicroPulser” electroporator (BioRad, Hercules, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... About 0.5 μg of plasmid DNA were mixed with the cell suspension and transferred to ice-cold 1mm gap cuvettes (Bio-Rad). Cells were electroporated in a Bio-Rad Gene Pulser Xcell system using settings ...
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bioRxiv - Developmental Biology 2021Quote: ... or with ACVR1 ECD plasmid DNA that was precipated onto gold beads and administered using a HELIOS Gene Gun (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 5% sorbitol synchronized ring stage Pf NF54 parasites at 5-8% parasitemia were electroporated with 100 µg of pFC-LINUP plasmid at 0.31 kV and 950 µF using a BioRad Gene Pulser (BioRad, Hercules, CA). Positive selection with 8 nM WR99210 (WR ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cell Biology 2023Quote: ... 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress, 45-0801) with 5-10 µg of plasmid DNA and electroporated with the Gene Pulser Xcell System (Bio-Rad) using 2 pulses at 200 V for 5 ms ...
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bioRxiv - Molecular Biology 2024Quote: Samples in 1X Laemmli loading buffer (generally representing 1-2 µL of replication reaction or 9 µL of a plasmid pulldown sample) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... were split into 24-well plates at 90.000c/ well and after 24h the indicated plasmids were transfected using TransFectin Lipid reagent (Bio-Rad 1703352) at a total of 50-66ng/well ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...