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Citations for Bio-Rad :
301 - 350 of 582 citations for N 3R Pyrrolidin 3 ylmethyl acetamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg of protein was separated on 4% to 20% precast polyacrylamide gels (Bio-Rad, cat # 4561095) for detection of histone H3 andH3K27me3 ...
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bioRxiv - Biochemistry 2020Quote: ... The purified MCU-EMRE subcomplex in DDM was then reconstituted into nanodisc by incubating with Msp1 protein, lipids (POPC:POPE:POPG, 3:1:1 molar ratio) and BioBeads (BioRad) at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were again washed 3 times in PBST before being incubated with Clarity™ ECL reagents (Bio-Rad) or SuperSignal™ West Femto Maximum Sensitivity Substrate and visualized (see S11 Fig for full length immunoblots).
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories). Protein was transferred to a nitrocellulose membrane (Hybond ECL ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed 3 times in TBSt and then incubated with HRP secondary antibodies (anti-Mouse HRP, Biorad, 170-6516 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBST (3 times) and developed with ECL western blot substrate (Bio-Rad) and imaged using Azure C300 imaging system (Azure biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: 4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from two reactions were collected and separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
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bioRxiv - Molecular Biology 2024Quote: ... Plates were incubated for 3 days at 30 °C and imaged in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Immunology 2024Quote: ... cDNA was mixed with appropriate primers (Supplementary Table 3) and SYBR green master mix (BioRad, catalog no. 1708882) and run on a Thermocycler T100 (BioRad) ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Molecular Biology 2023Quote: ... we performed 3 washes with PBST for 10 minutes before imaging the membrane on a ChemiDoc system (BioRad). The original scans of the blots are provided in Supplemental Table 3.
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bioRxiv - Cell Biology 2022Quote: ... pH 7) and Western blotting samples were prepared by adding sample buffer (3× Laemmli Sample Buffer 1610747; BioRad) plus 3.57 M β-mercaptoethanol (2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... The purified 3-cycle PCR products were qPCR quantified using Platinum SuperFi II on a CFX96 system (BioRad). The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat ...
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bioRxiv - Plant Biology 2022Quote: ... 12.5 mM imidazole) for 3 times and boiled at 95°C with 30 μl SDS loading dye (BioRad). After removing the beads ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were blocked overnight at 4°C in PBS with 3% BSA and 10% donkey serum (Bio-Rad), stained with DAPI (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked in 3% nonfat dry milk in TBST (Blotting-grade blocker nonfat dry milk; Bio-Rad) at room temperature for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... Only clones verified from both upstream and downstream genome-insert junctions were further analyzed for mCherry copy number by ddPCR (CN < 1.5 = single copy per genome, CN ≥ 2.5 = 3 or more copies per genome; Biorad). Then clones with 2 copies per genome had the target site amplified by BioTherm DNA polymerase (GeneCraft ...
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bioRxiv - Biochemistry 2024Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on the 4-20% Criterion TGX Stain-Free Precast gels (BioRad) in Tris/Glycine/SDS running buffer (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (Bio-Rad) after activation with ECL substrate (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Twenty-μg protein samples were separated by SDS-PAGE using a Mini-Protean 3 Cell system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... membrane was washed 3 x 10 min in PBS-T and imaged for chemiluminescence using ChemiDoc system (Biorad) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... was applied to the membrane for 3 min and then imaged using a chemiDoc imaging system (Bio-Rad). Image lab (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Pax-3 and Pax-7 were quantified through Real-Time PCR using SYBR® Green dye (BioRad, USA) (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2024Quote: ... at 90-120 Volts for 60-90 minutes in a Mini-Protean® 3 Cell (Bio-Rad Laboratories). Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... Gels with Young’s modulus of 1.2 kPa were made by preparing a solution of 3% acrylamide (Bio-Rad), 0.11% bisacrylamide (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3 times for 10 minutes each and coated with enhanced chemiluminescence (ECL; Clarity ECL, BioRad). Using X-ray film ...
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bioRxiv - Cell Biology 2020Quote: ... Cell cycle of cells (> 3 × 104) were determined by HOECHST fluorescence in single cell by ZE5 cell analyzer (BioRad) and data were processed by Flowjo.
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bioRxiv - Biophysics 2020Quote: ... the membrane was washed 3 times with PBST and incubated with an ECL Western blot Reagent (1705060, Bio-Rad), for 5 min in the dark ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel electrophoresis was performed using the BioRad Mini-PROTEAN 3 gel electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA). Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were subsequently washed 3 times in lysis buffer and bound complexes were eluted using 4x Lämmli sample buffer (Biorad). Samples were boiled at 95°C for 7 minutes and resolved through SDS-PAGE prior to Western blotting or analysed by quantitative Mass Spectrometry as described in the label-free LC-MS/MS analysis section ...
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bioRxiv - Biochemistry 2020Quote: ... using a DNA Engine Opticon 3 real time PCR system (MJ Research) with Opticon Monitor 3.1 analysis software (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then transferred semi-dry for 7 min at 1.3 mV using Trans-Blot Turbo 0.2 μm nitrocellulose transfer packs and the Trans-Blot Turbo transfer system (BioRad). Blots were blocked with Odyssey blocking buffer (LI-COR ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed 3 times washing in TBS+0.05%Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Cell Biology 2022Quote: ... Samples were subjected to SDS-PAGE by resolving on a 3-8% CriterionTM XT Tris-Acetate gel (Bio-Rad) for immunoblotting.
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Developmental Biology 2022Quote: ... 1970) and the equivalent of 0.5 - 3 oocytes/embryos were electrophoresed on SDS-PAGE TGX AnyKD Ready Gels (BioRad) or large homemade gels and transferred to nitrocellulose (Power Blotter ...
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bioRxiv - Bioengineering 2021Quote: ... All electrochemical measurements were done in a 3 electrode configuration with a graphite rod counter electrode (BioRad Laboratories, Inc.) and a Ag/AgCl reference electrode (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Transcriptionally active Q0.3 fraction (0.32–0.4 M) were pooled and applied directly to hydroxyapatite (HAP) type II ceramic resin (Bio-Rad), washed first at 0.38 M ...
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bioRxiv - Cell Biology 2022Quote: ... All reactions were performed in 3 technical replicates using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Data was analysed according to the 2-ΔΔCT method65.
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at the adequate temperature during 2-3 days and photographed with the Chemiluminiscent Imager (Bio-Rad). For methyl methanesulfonate (MMS ...
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bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNAs of each sample were resolved on 10% Criterion TBE-urea polyacrylamide gel (Bio-Rad), and processed with the standard procedure described previously (63) ...