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Citations for Bio-Rad :
301 - 350 of 5646 citations for 7 Amino 8 oxo 3 cis prop 1 enyl 5 thia 1 azabicyclo 4.2.0 oct 2 ene 2 carboxylic acid diphenylmethyl ester hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... pH 7) and Western blotting samples were prepared by adding sample buffer (3× Laemmli Sample Buffer 1610747; BioRad) plus 3.57 M β-mercaptoethanol (2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Microbiology 2019Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (BioRad), 1 μl of 20× WT primers/probe ...
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bioRxiv - Cell Biology 2020Quote: ... fixed in 4% paraformaldehyde for 2 h at 4 °C and embedded in PBS containing 3% low-melting point agarose (Bio-Rad, California, USA). The agarose gels containing the fixed organoids were processed in a standard automated tissue histology processor ...
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bioRxiv - Biochemistry 2023Quote: ... pre-run for 1 hour at 4 ° C and run for 1 hour at 120 volts on Mini-Protean 3 gels (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... This step was repeated for 3 times and the samples were concentration-matched at OD500nm = 10 and mixed 1:1 with 2x Laemmli buffer (Bio-Rad), containing SDS and 2-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Glucagon-like peptide 1 (GLP-1) and Adiponectin were measured using quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) and Bio-Plex Pro Mouse Diabetes Adiponectin assay #171F7002M (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... build 7 (BioRad). Primary antibodies were as follows ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated for 1 h with 5% blotting grade blocker (Bio-Rad). Primary antibodies were incubated overnight at 4℃ and secondary HRP-conjugated antibodies were incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... After 1 h blocking (5% non-fat milk, Bio-Rad 170–6404) at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (Bio-Rad), 1 μl of 20× WT primers/probe ...
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bioRxiv - Microbiology 2019Quote: ... Each PCR was performed in a total volume of 10 µL containing 5 µL of 2 × iQSYBR Green supermix (Bio-Rad, USA), 200 nM of each primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... to bis-acrylamide (2% w/v; Bio-Rad) was varied to tune gel stiffness to 20 kPa ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). The Q-PCR primer sequences are in Additional file 8 ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). The Q-PCR primer sequences are included in Additional file 8.
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bioRxiv - Biochemistry 2020Quote: ... SM-2 Bio-Beads was from Bio-Rad Lab. ...
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bioRxiv - Biochemistry 2020Quote: ... SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl of 2×ddPCR Supermix (Bio-Rad), wild-type and mutant allele-specific TaqMan probes at a concentration of 0.25 μM ...
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bioRxiv - Biophysics 2020Quote: ... Then activated Bio-Beads SM-2 (Bio-Rad), ∼300 uL ...
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bioRxiv - Microbiology 2022Quote: 2 mm Electroporation Cuvettes (Bio-Rad, catalog #: 1652092).
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bioRxiv - Immunology 2019Quote: ... 2% low melting agarose (CleanCut agarose, Bio-Rad) was melted at 70 °C followed by incubation at 43 °C for 10 min ...
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bioRxiv - Bioengineering 2019Quote: ... and 2%bis-acrylamide (Bio-Rad 161-0142) were mixed with ammonium persulfate (APS ...
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bioRxiv - Microbiology 2020Quote: ... and CD16-FITC (clone AT154-2, Bio-Rad) in RPMI 1640 media for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... A 2 % Agarose gel (Bio-Rad, California, USA) was prepared ...
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bioRxiv - Molecular Biology 2020Quote: ... pre-equilibrated Bio-Beads SM-2 (Bio-Rad) was added in batch and the sample was mixed end-over-end overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). Q-PCR primer sequences as follows ...
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bioRxiv - Cell Biology 2020Quote: ... 2% v/v β-mercaptoethanol (Bio-Rad, 1610710) and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2-mercaptoethanol (Bio-Rad Laboratories B.V.) and incubated at 100°C for 6 min ...
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bioRxiv - Genomics 2021Quote: ... 2% low melting agarose (CleanCut agarose, Bio-Rad) was melted at 70 °C followed by incubation at 43°C for 10 min ...
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bioRxiv - Genomics 2021Quote: ... 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... Damp Bio-Beads SM-2 resin (Bio-Rad) was added to the mixture for 3 h at 4°C ...