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Citations for Bio-Rad :
301 - 350 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C in a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 3% non-fat dry milk in TBS-T buffer for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and cooled to 4°C using a thermal cycler (Biorad). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... hold at 4°C in a C1000 Thermal Cycler (Biorad). We then performed RT-qPCR for gene expression quantification of the following genes ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C in a S1000 thermal cycler (Bio-Rad). The starting volume of each reaction was 100 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... Precision Plus Protein Western C Standards (Bio-Rad, 161-0376) were used to determine molecular weights ...
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bioRxiv - Neuroscience 2024Quote: ... held at 4 °C (C1000 Touch Thermal Cycler; Bio-Rad). Preamplified cDNA was immediately diluted 1:5 with 20 uL DNA Suspension Buffer and stored at –20 °C until use.
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bioRxiv - Microbiology 2023Quote: ... and held at 4 °C in a thermocycler (Bio-Rad). The newly synthesized 20 µL cDNA reactions were diluted 1:5 with 80 µL sterile ...
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bioRxiv - Cancer Biology 2021Quote: After running through 8% SDS-polyacrylamide (SDS-PAGE; made in house) by gel electrophoresis (BioRad, Hercules, CA, USA), the proteins were transferred ...
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bioRxiv - Microbiology 2019Quote: ... Total cell lysates were separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with TBST with 5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... were resolved on 8-12% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose blotting membranes (Bio-Rad), following standard procedures ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini-Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... were loaded onto an 8-channel DG8TM cartridge for droplet generation in the QX200 Droplet Generator (Bio-Rad). Droplets were transferred into a 96-well plate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... SDS-PAGE was performed using 8-16% gradient gel and transferred to nitrocellulose paper using the Transblot (BioRad). Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
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bioRxiv - Genomics 2020Quote: ... quantifying 8 mRNAs and 10 circRNAs of interest with the SsoAdvanced™ Universal SYBR Green Supermix (BioRad, USA). All reactions were performed in 384-well plates on the LightCycler480 instrument (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... samples were buffer exchanged into 150 mM of ammonium acetate pH 8 using Bio-Spin P6 columns (BioRad) and the final protein concentrations were adjust to 1-5 µM ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini- Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... 8 ng of granuloma protein was loaded per lane into Any kD Mini-PROTEAN TGX gels (Bio-Rad), separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... serum insulin was performed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Bio-Rad, Hercules, CA) as per the manufacturer’s protocol.
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bioRxiv - Physiology 2022Quote: ... The 8% equivalent of each fraction by volume was run on a 4-15% TGX gel (Bio-Rad) for Western blotting using affinity-purified polyclonal rabbit anti-mouse Prdm13 ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 μl from each sample were loaded onto Mini Protean TGX Pre-cast 4-15% gels (Bio-Rad) and migration was carried out for approximately 45 min at 150V ...
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bioRxiv - Molecular Biology 2023Quote: 4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... separated on 6% acrylamide 8 M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad laboratories) by electroblotting ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Genomics 2023Quote: ... the ligation products were amplified by 8 cycles of PCR on an S1000 thermocycler (Bio-Rad, Hercules, CA) using Q5 High-Fidelity PCR master mix with HF buffer (NEB Ipswich ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from two reactions were collected and separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
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bioRxiv - Biophysics 2024Quote: ... 20 µL of the supernatant was taken and added to 8 µL 4X Laemmli sample buffer (Bio-Rad) and 12 µL of 8M urea ...
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bioRxiv - Molecular Biology 2021Quote: ... The qRT-PCR was performed in a 2-step amplification with 40 cycles of 95 °C for 30 s and 55 °C for 30s using a Bio-Rad IQ thermocycler (Bio-Rad Laboratories Inc. Hercules, CA). Each reaction was loaded on a 384-wells standard qPCR plate and ran in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Neuroscience 2021Quote: ... Lcn2 and C3 were quantified by quantitative real-time PCR (qRT-PCR) using the QuantStudio 7 Pro System thermo-cycler (ThermoFischer Scientific) and SYBR Green Master Mix (Bio-Rad Laboratories). Data are shown as fold change relative to control samples using the ΔΔCq method with Rpl4 as an internal control gene ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time PCR was performed with the Applied Biosystems ViiA 7 system using iTaq Universal SYBR Green Supermix (Bio-rad #1725121). Human actin was used as a house keeping control ...
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Genomics 2019Quote: ... Resulting single-cell GEMs were collected at the completion of the run (~7 min) and linear amplification was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; 1851197): 72°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... and image captured by continuous exposure for 7 minutes with a Bio-Rad ChemiDoc MP Imaging System (12003154, Bio-Rad, CA, USA). The protein expression was quantified by determining individual band density using ImageJ (FIJI ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
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bioRxiv - Immunology 2021Quote: ... Settings for analysis using a C-1000 CFX96 machine (Bio-Rad) were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... overnight at 37 °C in a G1000TM Thermal cycler (BIO-RAD) to digest trehalose to produce free glucose ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:500), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Microbiology 2019Quote: ... 72°C,20"]x45 using SSO Advanced SYBR Green Supermix (BioRad), and 300 nM Tox9 and 11 primers listed in Table S1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200 g for 2 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... at 75°C for 10 minutes (T100 Thermal Cycler, Bio-Rad). The denatured proteins were pelleted through centrifugation at 3,200g for 2 minutes.
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bioRxiv - Physiology 2023Quote: ... and transferred (100V, 1h, 4°C) onto PVDF membranes (Bio-Rad). Blots were blocked in buffer containing 5% BSA (Cat#A9418 ...
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bioRxiv - Biochemistry 2023Quote: ... 59 and 61°C in an S1000 thermal cycler (Bio-Rad). For CETSA with dose-response ...