Labshake search
Citations for Bio-Rad :
301 - 350 of 8069 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
-
bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... qPCR reactions were prepared from cDNA at 1:5 dilution with SYBR Green iTaq Universal SYBR Green Supermix (Biorad, 1725120) and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad ...
-
bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
-
bioRxiv - Microbiology 2024Quote: ... Blots were then treated with either 1:10,000 dilutions in 5% milk/TBS-T of goat anti-mouse IgG StarBright700 (BioRad, #12004158) or goat anti-rabbit IgG IRDye800CW (Fischer ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were diluted in skim milk 5% in TBS-T: goat anti-mouse HRP (1:10.000, 1721011 Bio-Rad).
-
bioRxiv - Cell Biology 2024Quote: ... Digested DNA was purified and 2–5 μg of DNA was run on a 1% PFGE agarose gel (Bio-Rad) in 0.5 × TBE buffer using the CHEF-DRII system (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
-
bioRxiv - Biophysics 2022Quote: ... 1 mg of post-thrombin digested claudin-4 in DDM was treated with 4 mg of amphipol (1:4 w/w) for 2 hours before removing detergent via addition of 400 mg SM-2 biobeads (Bio-Rad). Excess cCpE was added to each claudin-4 sample at a molar ratio of 1:1.5 ...
-
bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
-
bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
-
bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
-
bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...
-
bioRxiv - Microbiology 2020Quote: The supernatants from the different lysis buffer extractions described above were diluted 1:4 in 4x Laemmli sample buffer (Bio-Rad) supplemented with beta-mercaptoethanol following manufacture’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was carried out using 4 μl cDNA (diluted 1:20) with iQ SYBR Green Supermix (BioRad, CA, USA) and specific primers (Biolegio ...
-
bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
-
bioRxiv - Molecular Biology 2023Quote: RT-qPCR was employed to find the levels of quantification of mucin 1 and 4 mRNA transcripts according to protocol of the Real-Time PCR system (BioRad./ USA)[39] ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
-
bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
-
bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
-
bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
-
bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
-
bioRxiv - Physiology 2024Quote: ... were diluted in 2% BSA and membrane were incubated for overnight at 4°C followed by HRP-conjugated secondary anti-rabbit antibody (1:5000, cat# 1706515, Biorad, USA) incubation for 1hr at room temperature ...
-
bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
-
bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were incubated overnight at 4°C in blocking solution 5% (w/v) Blotting-Grade Blocker (BioRad) in PBS-Tween buffer (1× PBS ...
-
bioRxiv - Cell Biology 2022Quote: Equal volumes (5 µg) of the prepared co-IPs were separated by SDS-PAGE (4–15%, Bio-Rad) and transferred to PVDF membranes (Millipore) ...
-
bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C and separated on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins could be detected with an infrared imager (Odyssey ...
-
bioRxiv - Molecular Biology 2023Quote: ... heated at 95°C for 5 min then run on a 4-12% Bis-Tris gel (BioRad 3450125). The input sample is identical to the initial solution containing protein in 60 μL 1x selection buffer prior to capture with 20 passages over the ME200 tip ...
-
bioRxiv - Neuroscience 2023Quote: ... with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094). The gels were run in TGS buffer (1x ...
-
bioRxiv - Biochemistry 2024Quote: ... boiled for 5 min and then resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
-
bioRxiv - Systems Biology 2023Quote: ... F4/80 (Bio-Rad, clone: Cl:A3-1, 1:80), TIM4 (BioLegend ...
-
bioRxiv - Microbiology 2024Quote: ... anti-WC-1 (Bio-Rad, MCA838GA, dilution 1:20) and anti-Pax5 (Agilent DAKO ...
-
bioRxiv - Neuroscience 2024Quote: ... Blots were blocked in casein PBS (1:1, Biorad) for 30 min at RT on shaker and then probed overnight at 4°C on shaker with primary antibodies (1:1,000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5’KI and 3’KI as detailed (Fig 2A and D) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... R primer: 5′-GTACCACCATGTACCCAGGC-3’) cDNA were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Cat.:# 170882) in a qPCR detection system (LightCycler LC480 ...
-
bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3 × 5min TBS-T and 2 × 5 min TBS) before being imaged on the ChemiDoc MP Imaging System (Biorad). Subsequently ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... 3-5 µL of cell suspension was then deposited on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) pad prepared using a gene frame positioned on a glass slide ...
-
bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
-
bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and electrophoresis in Tris-glycine buffer ...
-
bioRxiv - Microbiology 2023Quote: ... The gel was run at 125 V for 3 to 4 hours and the RNA was then transferred to a Zeta-Probe GT membrane (Bio-Rad) by capillary action with 20 x SSC buffer (3 M NaCl ...
-
bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...