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Citations for Bio-Rad :
3401 - 3450 of 4472 citations for Rat Tsukushin Tsku ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 300-400 ng of cDNA from VP was synthesized using the reverse transcriptase iScript cDNA synthesis kit (Bio-Rad). mRNA expression changes were measured using qRT- PCR with PerfeCTa SYBR Green FastMix (Quanta) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was prepared from 10 to 50 ng RNA according to instructions of iScript™ cDNA Synthesis Kit (BioRad). Qiagen RNeasy® Plus mini kits (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated anti-rabbit and anti-mouse were used for detection using the chemiluminescence detection kit Clarity ECL (Biorad).
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a Bio-Rad DC™ protein assay kit (Bio-Rad, Marnes-La-Coquette, France). Proteins (30 µg ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted as described above and cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad). PCR was performed using Bio-Rad CFX96 Real-Time PCR systems ...
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bioRxiv - Plant Biology 2021Quote: ... The iScript™ cDNA synthesis kit and iTaq universal SYBR green PCR master mix were obtained from Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... were detected by staining membranes with anti-mouse IFN-γ biotin (1μg/ml) (R46A2) followed by streptavidin-Alkaline Phosphatase (1μg/ml) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Microbiology 2021Quote: ... Purified RNA (500 μg) was used to synthesize cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Pre Developed Taqman® (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 μl sample lysate ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was then quantified by qRT-PCR using the iTaq™ Universal Probes One-Step Kit (BIO-RAD) according to manufacturer’s protocol using a forward primer (5’-CCGCTGCCCAACACAAG-3’) ...
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bioRxiv - Microbiology 2021Quote: ... and amplicons were generated via qRT-PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD) according to manufacture protocol ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from RNA using Biogen iScript kit and qPCR was performed using either Sybr Green iTaq (BioRad) or TaqMan (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... an iScript reverse transcription kit was used to convert one microgram of RNA to cDNA (Bio-Rad, Hercules, CA). No reverse transcriptase controls were included ...
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bioRxiv - Cell Biology 2022Quote: ... 1000 ng RNA was reverse-transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-RAD, CA, USA). cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Plant Biology 2019Quote: cDNA was synthetised from 1μg of total RNA using the iscript Reverse Transcriptase Kit (Bio-rad Cat no 1708891) in 20 μl of final volume ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of solubilized protein was determined with a Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA USA). The retinal lysate with loading buffer was boiled for 3 min prior to loading onto an 8% sodium dodecyl sulfate (SDS)-polyacrylamide gel with the same amount of protein (5ug ...
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bioRxiv - Physiology 2019Quote: ... sonicated for 20 seconds and protein concentration was determined with the commercial kit (DC™ Protein Assay; Bio-Rad). Fifteen µg of proteins were reduced with modified Laemmli buffer (40 mM Tris pH 6.8 ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Synthetic Biology 2019Quote: ... Protein concentrations in the resuspended samples were quantified using a DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA). After quantification ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed on 1 μg of purified RNA using the iScript Reverse Transcription Supermix kit (Bio-Rad). The cDNA was then added to SsoFast (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... RNA integrity was assessed by microfluidic capillary gel electrophoresis using the ExperionTM RNA HighSens Analysis Kit (#7007105, Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... 1µg of purified RNA was used to make cDNA using the iScript™ cDNA synthesis kit (Bio-Rad, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed to cDNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Quantitative PCR was performed on a Bio-Rad CFX96 real-time system using SsoFast Evagreen mix (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Neuroscience 2021Quote: ... We used 1µg of RNA to produces cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). Primers used for RT-PCR are listed in Table 1.
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from plants using the Aurum Total RNA kit according to the manufacturer’s instructions (Bio-Rad). First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: Genomic DNA for optical mapping was extracted from agarose-embedded specimens using the CHEF Genomic Plug DNA kit (BioRad) in order to minimise fragmentation ...
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bioRxiv - Plant Biology 2021Quote: ... 1μg of total RNA were used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-rad Laboratories, India). For quantitative real-time PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of all fractions was quantified using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized from 0.5 μg of total RNA using the iScript Advanced cDNA synthesis kit (Bio-Rad; 1725037) following the protocol 25°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and loaded onto 10% SDS-PAGE gel (TGX™ Fast Cast™ Acrylamide Kit 10%, BioRad, California, United States). Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad) ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA (0.1 – 1.0 µg) with iScript Advanced cDNA Synthesis Kit (BioRad, Hercules, CA, USA). QRT-PCR was performed using PowerUp SYBR Green Master Mix reagent (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad, Hercules, CA, USA) and 10μM primers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: The qPCR mixture of 10 μl was prepared using the iQ™ SYBR® Green PCR Kit (Bio-Rad): 5 μl of 2x iQ™ SYBR® Green Master Mix ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted and reverse-transcribed according to the manufacturer’s protocol (iScript™ cDNA Synthesis Kit, BIO-RAD). cDNA was loaded in duplicate and qPCRs were performed using the BIO-RAD CFX System (BIO-RAD ...
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bioRxiv - Genomics 2021Quote: The single nucleus ATAC-seq experiment was performed using the SureCell ATAC-Seq Library Prep Kit (Bio-Rad, 17004620) following the manufacturer’s protocol for the OMNI-ATAC version (45) ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... 1μg of purified RNA was used to make cDNA using the iScript™ cDNA synthesis kit (Bio-Rad, USA) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... or 15% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45μM LF PVDF Transfer Kit (Bio-Rad). Membranes were incubated for 1h at room temperature with blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion of genomic DNA was carried out using Turbo DNase treatment (TURBO DNA-free™ Kit, Bio-Rad, USA) per the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...