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Citations for Bio-Rad :
3401 - 3450 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were purified by Ni-charged Profinity IMAC resin (BioRad) and purity was analyzed by SDS-PAGE and concentration estimated by the Bradford assay (Bradford ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Immun-Blot PVDF membranes (Bio-Rad) and then blocked with 5% milk in tris-buffered saline with 0.1% Tween (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... After the proteins were transferred to nitrocellulose membrane (BIO-RAD), the membranes were blocked for 1 h in TBST containing 5% bovine serum albumin.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto PVDF membranes (Bio-RAD, 1704156) using the Turbo-Blot transfer system ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant used following protein quantification (Bio-Rad #5000111).
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bioRxiv - Microbiology 2024Quote: ... proteins were transferred to a nitrocellulose membrane (Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories) ...
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bioRxiv - Bioengineering 2024Quote: ... Protein bands were visualized with Coomassie Brilliant Blue R250 (BioRad).
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bioRxiv - Biochemistry 2024Quote: ... After protein quantitation using a Bradford reagent (Bio-Rad #5000205), samples were mixed with Laemmli sample buffer (Bio-Rad #161-0747 ...
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bioRxiv - Molecular Biology 2024Quote: ... Precision Plus Protein Kaleidoscope Standards (Bio-Rad, Hercules, CA, USA) were used for molecular weight determinations ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to Immun-Blot PVDF membrane (Bio-Rad) for 120 minutes at 100 volts then blocked for one hour in 3% BSA TBS-T buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred (Trans-Blot Turbo, Bio-Rad; cat# 1704158) to a nitrocellulose membrane (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein concentrations were determined using the DC assay kit (BioRad) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and loaded onto Criterion TGX stain-free protein gels (Biorad). After electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... protein concentration was determined by Bradford colorimetric method (BioRad #5000006). Total proteins (20 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred to a nitrocellulose membrane (Bio-Rad) and subsequently detected by anti-GFP rabbit polyclonal antibody (Novus Biologicals #NB600-308 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was estimated using a Bradford assay (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... 10% mini-protean TGX precast protein gel (4561033, Bio-Rad); protease and phosphatase inhibitor cocktails (PPC1010 ...
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bioRxiv - Biochemistry 2024Quote: ... 4–20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad), 4–20% Criterion TGX Stain-Free Protein Gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by using Bradford assay (Bio-Rad). Tag-free Mical1 full-length bMERB domain ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to 0.2 μm nitrocellulose membranes (Bio-Rad), blocked for 1 hr at RT in 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad, 1704159) using the Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Relative protein levels were quantified using Image Lab (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was quantified using a Bradford assay (Bio-Rad). Samples were separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Protein quantitation was assessed by Bradford assay (Bio-Rad #5000006) using serial dilutions of bovine serum albumin (BSA ...
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bioRxiv - Synthetic Biology 2024Quote: ... along with a Precise Plus protein standard ladder (Bio-Rad). 14 μL of each cell lysate sample (1.3 μg of soluble protein ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were resolved by 4-15% gradient SDS gels (Biorad). The gels were dried and exposed to a phosphorimage screen for 24 hrs before being developed on the Typhoon FLA7000 (Cytiva).
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bioRxiv - Genomics 2024Quote: ... Protein concentration was determined using Bradford assay (Biorad 500-0006). 1-2 mg of protein extract was used per ChIP and 5% was taken out to use as input DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected using enhanced chemiluminescence substrate (107- 5061, BioRad). Images were acquired using detection systems Amersham ImageQuant 800 (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... or a semi-dry protein transfer system from BIO-RAD and analyzed by Western blotting ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Total RNA was isolated from the prelimbic and infralimbic region of the mPFC by single-step guanidinium isothiocyanate/phenol extraction using PureZol RNA isolation reagent (Bio-Rad Laboratories; Segrate, Italy), according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Total RNA was isolated from the prelimbic and infralimbic region of the mPFC by single-step guanidinium isothiocyanate/phenol extraction using PureZol RNA isolation reagent (Bio-Rad Laboratories; Segrate, Italy), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) reference gene RNA level was performed to determine the efficiency of RNA extraction and qPCR amplification (Bio-Rad PrimePCR assay #qHsaCED0038674). SARS-CoV-2 viral loads below 40 RNA copies/mL were categorized as undetectable and set at 1.0 log10 RNA copies/mL.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated by a single step of guanidinium isothiocyanate/phenol extraction by using a PureZol RNA isolation reagent (Bio-Rad Laboratories, Segrate, Italy) according to the manufacturer’s instructions and quantified by spectrophotometric analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation for the protein lysate was performed by using SureBeads™ Protein G or A Magnetic Beads (purchased from Bio-Rad; catalog numbers1614023 and 1614013). Western blotting experiments were performed by using precast TGX 4-20% gels (purchased from Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... and then converted to units of fmol/mg of total protein added to an assay point (as measured by Bio-Rad DC Protein Assay Kit). Equilibrium dissociation constants (Kd ...
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bioRxiv - Neuroscience 2020Quote: ... Uptake was normalized to total mg of protein per sample measured via Bio-Rad DC protein assay kit (5000112 Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2019Quote: ... Protein content of muscle homogenate was measured in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA) against bovine serum albumin standards (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad, Hercules, CA, Catalog #5000006).
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations were analyzed using a Quick Start Bradford Protein Assay Kit with bovine serum albumin as a standard (Bio-Rad Laboratories, Hercules, CA, USA). The samples were standardized by diluting in the diluent ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting total protein was quantified using the Bio-Rad DC Protein Assay Kit according to the manufacturer’s recommended protocol (Bio-Rad, Cat#5000111, Hercules, CA, USA). Samples and standards were read on a BioTek Synergy HT plate reader after a 15 min incubation period ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Immunology 2021Quote: Proteins (20 µg per well) were separated in 4–15% Criterion™ TGX™ Precast Midi Protein Gels with 26 wells (Bio-Rad, Hercules, CA, USA) at a constant voltage of 120 V ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein estimations were performed using the Bio-Rad microtiter Bradford protein assay (California, USA) according to the instructions provided (Bio-Rad version: Lit 33 Rev C) and measurements were made at 595 nm using a SpectraMax Plus ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were adjusted to 5 µg of total soluble proteins in native sample buffer and then loaded onto a 4-20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, Cat. No. 4561094). Protein electrophoreses were performed in TG buffer (60 mM Tris ...