Labshake search
Citations for Bio-Rad :
3401 - 3450 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Samples were loaded in precast 4-20% gradient sodium dodecyl sulfate (SDS) polyacrylamide gels (Mini-PROTEAN TGX gels, Bio-Rad) and run at room temperature with 100 V (constant ...
-
bioRxiv - Molecular Biology 2019Quote: ... The equal amount of lysate (25 μg) was loaded to each lane for SDS-PAGE (4-20% TGX gel, BioRad). The proteins were transferred to a PVDF membrane with wet-transfer method at 40V for 16 hours on ice with a transfer buffer (25 mM Tris ...
-
bioRxiv - Plant Biology 2019Quote: ... Purity and size of each peptide were determined by electrophoresis on a 4-20% Mini-Protean TGX gels (Bio-Rad). The correct mass of each peptide was confirmed by mass spectrometry prior to its use in experiments described below.
-
bioRxiv - Pathology 2019Quote: ... Approximately 50 μg of proteins from each sample were separated on 4–20% Mini-PROTEAN TGX precast gels (Bio-Rad) and transferred to a PVDF membrane.
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were subsequently blocked and permeabilised at room temperature for 30 minutes in PBS with 4% donkey serum (Bio-Rad) and 0.1% Triton X-100 (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2019Quote: ... Equal amount of cell lysates (10 μl) were loaded in 4–12% Mini-PrROTEAN TGX Precast protein Gels (Bio-rad) and transferred to PVDF membrane ...
-
bioRxiv - Systems Biology 2019Quote: ... Whole cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Native lysates were collected in a native sample buffer (50 mM tris ...
-
bioRxiv - Developmental Biology 2019Quote: Proteins were separated by SDS-PAGE on a 4-12% Novex gradient gel then transferred to nitrocellulose membrane with the Trans-Blot Turbo Transfer System (BioRad). Membranes were blocked in 50% Odyssey Blocking Buffer in 0.3% PBST (1x PBS with 0.3% Tween ...
-
bioRxiv - Microbiology 2019Quote: ... the remaining debris was sedimented by centrifugation and ten µL of each sample was loaded onto a 4-20% precast TGX protein gel (BioRad). Protein samples were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Biochemistry 2019Quote: ... 10% of 2 mM EGTA eluates were concentrated and separated on 4%– 20% gradient SDS-polyacrylamide Tris-glycine gels (Biorad). Total protein content was visualized by silver staining ...
-
bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to myosin II was estimated by a gel-shift assay (4–15% gradient gel, Biorad) and determined as >99% for both S1 and lever-arm-less S1 (Supplementary Fig ...
-
bioRxiv - Cancer Biology 2019Quote: ... Lysates were reduced in LDS with beta-mercaptoethanol and then polyacrylamide gel electrophoresis was performed on 4-20% Protean Mini TGX gels (Biorad) and transferred to Immobilon PVDF membranes for 15 minutes using mini TGX settings on the Trans-Blot-Turbo system (Biorad) ...
-
bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted directly in sample buffer and samples were separated on a 4-12% Criterion Bis-Tris gel (BioRad). Proteins were transferred to PVDF membrane (Immobilon-FL ...
-
bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: Nucleosome fractions containing 200 ng DNA were applied for SDS-PAGE with 4-20 % gradient SDS-PAGE gel (Bio-rad). Proteins were transferred to the nitrocellulose membrane (GE ...
-
bioRxiv - Genetics 2020Quote: Equal volumes of lysate from each fraction were separated by SDS-PAGE using 4-20% TGX Stain-Free mini gels (BioRad). Protein was transferred to PVDF membrane using the TransBlot Turbo system (BioRad) ...
-
bioRxiv - Biophysics 2020Quote: ... Western blot assays were performed after resolving the samples on a 4-15 % Tris-HCl gradient gel (Bio-Rad Laboratories).
-
bioRxiv - Bioengineering 2020Quote: ... Specific volumes of samples (described previously) were loaded on a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA) and run at 180 V for 45 min ...
-
bioRxiv - Cell Biology 2019Quote: ... SDS dye was added to a 1X concentration and lysates (equal µg of protein) were loaded onto 4-20% Tris-glycine gels (BioRad). Gels were transferred to nitrocellulose ...
-
bioRxiv - Physiology 2020Quote: ... Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad) and transferred to polyvinylidene diflouride (PVDF ...
-
bioRxiv - Physiology 2020Quote: ... equal amounts of protein (20 ug) were loaded in 4-12% - Criterion XT Bis-Tris protein gels (Bio-Rad, CA) and transferred to nitrocellulose membranes ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
-
bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
-
bioRxiv - Physiology 2021Quote: ... we ran the purified Hb fractions on a mini-protean precast 4-20% SDS PAGE gel (Bio-Rad, Hercules, CA), which we then stained with Coomassie brilliant blue-G ...
-
bioRxiv - Physiology 2021Quote: ... were handpicked to match size of islets across the samples and loaded into the polycarbonate perifusion chamber between two layers of polyacrylamide-microbead slurry (Bio-Gel P-4, BioRad) by the same experienced operator ...
-
bioRxiv - Developmental Biology 2019Quote: ... NuPage 4-12% Bis-Tris protein gels beofre transferring into PVDF membranes using the Trans-Blot Turbo transfer system (Biorad). Prior to blocking and antibody incubation ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 20 μL of the total crude cell lysate from each sample was loaded onto a 4–20% gradient Tris-HCl Criterion SDS-PAGE (BioRad). After transfer to polyvinylidene fluoride membrane ...
-
bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
-
bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
-
bioRxiv - Cell Biology 2019Quote: ... Immunoblotting was performed by standard methods using 4-15% Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad). Ponceau S staining of membranes was routinely used to assess equal sample loading and transfer efficiency ...
-
bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
-
bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed directly in sample buffer and protein extracts were separated on a 4-20% polyacrylamide gel (Bio-Rad). Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53 ...
-
bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
-
bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
-
bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
-
bioRxiv - Biochemistry 2021Quote: Eluted samples were resolved by SDS-PAGE using 4-20% gradient gels and then transferred to the nitrocellulose membrane using the Trans-Blot Turbo transfer system (Biorad). Following the blocking for 1 hour in blocking buffer (5% milk in TBST) ...
-
bioRxiv - Biochemistry 2021Quote: ... two probes (250 nM final each) (Supplementary Table 4) and template DNA were combined with ddPCR Supermix for Probes (No dUTP) (BioRad) in a final 20 μL reaction volume ...
-
bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
-
bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were separated according to size by SDS-PAGE in a Mini-Protean TGX stain-free 4-20% gradient gel (BioRad) and thereafter transferred onto a PVDF membrane ...
-
bioRxiv - Physiology 2021Quote: ... and proteins were stripped from beads by incubation in gel loading buffer with beta-mercaptoethanol and loaded to 4-15 % gels (Criterion; BioRad) for SDS-PAGE ...
-
bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant ~ 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL warm BHI medium was added and the cells were incubated at 30 °C for 6 h under shaking at 200 rpm and plated on BHI agar plates with 10 μg/mL erythromycin ...
-
bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
-
bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...