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Citations for Bio-Rad :
3301 - 3350 of 10000+ citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... with C1000/CFX96 RT-PCR System (Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... followed by RT-PCR (iQ SYBRgreen Supermix, Biorad) using the cycling conditions as follows ...
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bioRxiv - Microbiology 2019Quote: ... and a CFX96 RT-PCR system (Bio-Rad). Data analysis was performed with CFXManagerTM software (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Relative mRNA levels of the indicated genes were calculated by the 2-DDCT method (Bulletin 5279, Real-Time PCR Applications Guide, Bio-Rad), using GAPDH expression as endogenous control ...
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bioRxiv - Immunology 2021Quote: ... and the expression of specific genes was analyzed by SybrGreen-based real time PCR (iTaq Universal SYBR Green Supermix, BIO-RAD) on a CFX Connect Real-Time System (BIO-RAD).
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was done on a BioRad CFX96 Real Time PCR system with SsoAdvanced Universal SYBR Green Supermix (BioRad, Cat#170-8841) and primers detecting Vsx2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleavage-related change in fluorescence signal of the probe was measured every 30 s for 60 min under incubation at 37°C using a Real-time PCR system (Bio-Rad Laboratories ...
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bioRxiv - Synthetic Biology 2019Quote: ... The quality and quantity of the DNA in the retained library and file were measured by quantitative real time PCR (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... Fold difference in expression of mRNA at different stages were calculated by Ct method (Real-Time PCR applications guide Bio-Rad). The housekeeping gene SeryltRNA ...
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bioRxiv - Microbiology 2019Quote: DNA fragments that co-precipitated with H-NSHA were quantified by real-time quantitative PCR using SsoFast EvaGreen Supermix (Bio-Rad) according to the manufacturer’s instructions and using gene-specific primers ...
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bioRxiv - Immunology 2019Quote: ... at 10 pmol of each per reaction (Integrated DNA Technologies) by real-time fluorogenic PCR using SsoAdvanced Universal IT SYBR Green SMx (BIO-RAD) on a CFX Connect Real-Time System cycler (BIO-RAD) ...
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bioRxiv - Genetics 2019Quote: ... A total of 500 ng of RNA was used for cDNA synthesis (qScript cDNA Supermix, Quanta) and real-time quantitative PCR was performed (iTaq Universal SYBR Green Supermix, Bio-Rad) using CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Directed quantitative PCR was carried out on the Bio-Rad CFX Real-Time System with SYBR Green SuperMix (BioRad, Hercules, CA) as per the manufacturer’s instructions in a total reaction volume of 10 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... The comparative threshold cycle (Ct) method was used to determine relative transcripts levels (Bulletin 5279, Real-Time PCR Applications Guide, Bio-Rad), using β-actin expression as internal control ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme) on a CFX 96 Touch real-time PCR system (Bio-Rad). For YAP-TEAD inhibition studies ...
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bioRxiv - Biochemistry 2021Quote: ... The change in the fluorescence intensity during gradual unfolding owing to rise in the temperature is measured using CFX Real Time PCR instrument (Bio-Rad). The negative derivative of the change in fluorescence intensity with temperature was plotted against temperatures ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271, Bio-Rad) according to the manufacturer’s protocol (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was prepared and qPCR was carried out using iQ SYBR Green super mix and iQ5 multicolour real time PCR thermal cycler (Bio-Rad). The levels of mRNA expression relative to GS115 was normalized to 18S rRNA ...
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bioRxiv - Immunology 2022Quote: ... and the primers for murine IFNAR 1 and beta-Actin in a MyiQ™ Single-Color Real-Time PCR System (BioRad). Products of RT-PCR were separated by electrophoresis on a 2.5% agarose gel in 1x TBE buffer ...
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bioRxiv - Neuroscience 2021Quote: ... diluted cDNA or genomic DNA was subjected to quantitative real-time PCR in triplicate with the indicated primers using Ssofast EvaGreen Supermix (Bio-Rad) in a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Complementary DNA (cDNA) was used for real-time quantitative PCR using 2x iQ SYBR Green supermix and C1000 Thermal Cycler (Bio-Rad) using specific primers (see supplemental methods) ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 96-well transparent plate on a Bio-Rad CFX96 Real-Time PCR machine (Bio-Rad) in 10 µl mixtures each containing 5 µl of Takyon™ ROX SYBR® MasterMix dTTP Blue (Eurogentec ...
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bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo-Fisher) using the iTaq Universal SYBR green Supermix (Bio-Rad). The following primer sequences were used ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR (qPCR) using SYBR green reagent (iTaq™ Universal SYBR™ green Supermix, Bio-Rad, Hercules, CA, USA) was carried out using primers specific for 3 immune genes (Table 1) ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... the target transcripts were determined by qPCR using the PereCTa SYBR Green SuperMix (95055-500, Quanta Biosciences) on CFX96 real-time PCR system (Bio-Rad). The primer sequences are listed in Supplementary Table 2.
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bioRxiv - Microbiology 2019Quote: ... SYBR Green fluorescence from each qRT-PCR reaction was monitored using the C1000 Thermal Cycler with CFX96 Real-Time System (Bio-Rad). Relative abundance of transcripts was calculated relative to gyrA using the formula Transcript Abundance = PCR efficiency−(Ct[gene]-Ct[gyrA] ...
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bioRxiv - Plant Biology 2020Quote: ... Using the published Minimum Information for Publication of Quantitative Real Time PCR Experiments (MIQE Guidelines) and guidelines provided by Bio-Rad, primers were designed for these 6 genes using NCBI’s Primer BLAST program (http://www.ncbi.nlm.nih.gov/) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271, Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time quantitative PCR (qPCR) was performed using SsoAdvanced Universal SYBR Green Super-mix (Catalogue #172-5270, Bio-Rad, Hercules, CA) on a Bio-Rad CFX96 C1000 Touch Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... The 96-well plate was run on a C1000 Thermal Cycler CFX96 Real-time System PCR machine (Bio-Rad, Kidlington, UK). The CFX manager software (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using 10-20 ng cDNA as template and gene specific primer pairs shown in Table S8 in CFX Connect Real-Time PCR System (Bio-Rad). The PCR conditions were 95◻°C for 15◻min ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was conducted using primer sets for the indicated transcripts and iTaq Universal SYBR Green master mix (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... For qRT-PCR the indicated cDNAs of interest were amplified using PerfeCTa SYBR Green FastMix (Quantabio) in a CFX Opus 96 Real-Time PCR Instrument (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Cell Biology 2022Quote: ... and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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bioRxiv - Genetics 2022Quote: ... Samples were manually pipetted in 384-well plates (Framestar 480/384, 4ti-0381 4titude) and run in a CFX-384 Real-time PCR system (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCRs were performed in 384-well plates using 2x SYBR Green Mix and the CFX384 Real-Time System (Bio-Rad). Reaction mix included 3 µl of cDNA (diluted 1:5 in DEPC H2O) ...
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bioRxiv - Microbiology 2022Quote: ... Gene expression analysis was carried out using the PerfeCTa SYBR® Green SuperMix (Quantabio) and CFX96 Touch Real-Time PCR system (Biorad). Primers used for the qRT-PCR analysis are listed in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem) using iTaq Universal SYBR Green Supermix (Bio-Rad). Expression of lncRNAs was normalized based on 3 house-keeping gene ...
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bioRxiv - Microbiology 2022Quote: ... Plasma viral load was measured using qRT-PCR on a C1000 Thermal Cycler and the CFX96 Real-Time system (Bio-Rad) with 100 copies/ml limit of detection.
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bioRxiv - Plant Biology 2022Quote: ... and qPCR was performed using the SYBR Green Realtime PCR Master Mix (TOYOBO) on a CFX real-time machine (Bio-Rad). The PP2AA3 gene was used as the reference gene for normalization ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were amplified using a CFX96 Real-Time PCR System and threshold cycles were measured using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Dissociation curve analysis was performed to verify single products for each reaction ...
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bioRxiv - Microbiology 2022Quote: ... All qPCRs were carried out on a CFX96 Real-Time PCR system with the corresponding Bio-Rad CFX Maestro Software (BioRad, Australia). Cycling conditions for all three assays consisted of an initial denaturation at 95°C for 1 min followed by 40 two-step cycles at 95°C for 15 sec and 60°C for 30 sec ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed with 10-fold diluted cDNA using The SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad). The mean and standard error were determined using three biological replicates with three technical replicates each ...
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bioRxiv - Bioengineering 2024Quote: ... by quantitative real-time PCR using primers that amplify the bGH PolyA sequence (Table S3) and the SsoFast Evagreen mix (Bio-Rad). The virus was stored at -80°C.
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bioRxiv - Biochemistry 2024Quote: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was quantified by qPCR with a QuantStudio3 Real-Time PCR machine and transcript-specific primers and iTaq UniverSYBR Green Supermix (Bio-Rad). RNA was normalized to 18S rRNA levels and corrected for primer efficiency.
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad). The primers used for this assay are listed in SI Appendix ...