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Citations for Bio-Rad :
3301 - 3350 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: Western Blot samples of unbound fractions and IP were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... horseradish peroxidase (HRP) conjugated secondary antibodies (1: 5000) and signal detected using ClarityTM Western ECL Substrate solution (BioRad). Blots were visualised on a ChermiDOC XRS+ system (BioRad ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at RT and detected using Clarity or Clarity Max Western ECL Blotting Substrate (Bio-Rad) and imaged (Amersham AI600) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... NCBI accession DQ278441.1) (Table 1) were used for quantitative PCR using the iQ SYBR Green Supermix (BioRad, Canada) and the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 1 mg of RNA using the iScript cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... The membrane was incubated with a primary mouse anti-pig IL-18 antibody (diluted 1:500, Bio-Rad) or anti-β-actin antibody (diluted 1:500 ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription of 1 μg of RNA was carried out with iScript cDNA Synthesis Kit (1708891, Bio-Rad) using an extended transcription step of three hours at 42°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Physiology 2020Quote: ... and reverse transcribed into cDNA using 1 μg of RNA and iScript Reverse Transcriptase (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... incubated with primary antibody (monoclonal rat anti-F4/80, 1:200 dilution, MCA497; BIO-RAD; Hercules, CA, USA) for one hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10% (19:1) PAGE was prerun for 30 minutes at 100V in a Mini PROTEAN tank (Bio-Rad) electrophoresis chamber using 1X TBE (89 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM DTT) before being loaded on a HiTrapQ anion exchange column using the NGC FPLC (Bio-rad). PfQC was eluted using a salt gradient ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 ug of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Hydrogels of varying stiffness were prepared using varying ratios of 40% acrylamide to 1% bis-acrylamide (Bio-Rad) in a mixed solution with water ...
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were run for 1 hour 15 minutes at 150V in Criterion vertical cell electrophoresis device (Bio-Rad) and stained for 10-15 minutes using SYBR Gold (ThermoFisher) ...
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bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostaining was performed overnight at 4° C using primary antibodies for PTH (1:400, Bio-Rad, Hercules, CA), Cleaved caspase 3 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... intermedius overnight cultures were pelleted and resuspended in a 10:1 ratio of culture to InstaGene Matrix (BioRad) after which the extraction protocol as per manufacturer instructions was followed ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, 1708840). qPCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
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bioRxiv - Biochemistry 2023Quote: Reaction products were analysed using 20% denaturing polyacrylamide gel electrophoresis (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) containing 7 M urea (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: 1 μg TURBO DNase-treated RNA was reverse transcribed with the iScript cDNA reverse transcription kit (Biorad, 1708890) or 5 U AMV-RT (NEB ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized from 1 µg RNA with random hexamers using the iScript Reverse Transcription Supermix (Bio-Rad). Quantitative PCR was performed on a MyiQ (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The washed plugs were loaded in 1% Certified Megabase Agarose (2.4g agarose/240mL 0.5x TBE) (Bio-Rad, #1613108). CHEF-gel electrophoresis was performed on a CHEF Mapper System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... and a 1: 2000 dilution of secondary goat anti-rabbit horseradish peroxidase antibody (Bio-Rad Laboratories, Hercules, CA) were used to detect GFP.
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: ... Following measurement of the protein concentration using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG clone 1/27 was coupled to Affi-Gel 10 resin following the manufacturer’s instructions (Bio-Rad). A549 cells were inoculated at an MOI of 0.2 with S009 PB2-FLAG or S009 PB2-627K-FLAG in a 10 cm dish ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 12% SDS-polyacrylamide (29:1) or 4-15% precast polyacrylamide mini-gels (Bio-Rad) and electrotransferred on nitrocellulose membranes ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (1 μg) was used to generate the ProtoScript II First Strand cDNA Synthesis Kit (Biorad E6560S) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 1 ml HisTrap (Cytiva) column connected to NGC Quest 10 (Bio-Rad), previously equilibrated with Buffer A (25 mM Tris–HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... India (Table 1) on a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). The Bio-Rad CFX Maestro Software ...
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bioRxiv - Immunology 2023Quote: ... protein samples in assay-specific buffers were prepared with 1-2X final concentration of Laemmli buffer (Bio-Rad) with 2.5% β-ME and immunoblotted as described below ...
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bioRxiv - Genetics 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA by using an iScript kit (#1708891, Bio-Rad) on a Mastercycler (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation using the Gene Pulser Xcell (1700 V, 25 µF, 200 Ω, 1 pulse, Biorad) in C2989 5alpha electrocompetent bacteria (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were run on a 1% agarose gel and imaged using a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 µg of RNA per sample using the iScript cDNA Synthesis Kit (Bio-Rad) or iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Immunology 2023Quote: ... by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) on a real time thermal cycler CFX96 (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... and 1 μg of RNA was reverse-transcribed (RT) to cDNA using iScript cDNA synthesis kit (Bio-Rad) with oligo-dT primers for poly-A mRNA selection ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...