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Citations for Bio-Rad :
3251 - 3300 of 3508 citations for Norovirus GII.4 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The samples used for immunoblot analysis were separated by SDS-PAGE on a 4-20% gradient gel (Mini-PROTEAN TGX Gels, Biorad, Cat# 456-1093). Gel electrophoresis was performed at 120 V in running buffer (Tris-glycine ...
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bioRxiv - Neuroscience 2021Quote: ... Around 10-15 μl (around 20 μg of protein) were resolved by SDS-PAGE polyacrylamide gel electrophoresis using 4-20 % precast gradient gels (Bio-Rad, 456-1096), and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... and duck heads were fixed in 4% PFA overnight and stained for 20 minutes with 0.02% ethidium bromide (1610433, Bio-Rad, Hercules, CA, USA) using a previously published protocol (Eames and Schneider ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in Laemmli buffer and loaded into 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Cat #4561093). SDS-PAGE and protein blotting were performed according to (Dawe et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... were denatured and separated by gel electrophoresis using 4-15% linear gradient SDS-PAGE precast gels (Mini-Protean TGX Stain-free, Bio-Rad 456-8083). Following electrophoresis ...
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bioRxiv - Neuroscience 2019Quote: ... or 15 μg (for MATE1, MATE2, PMAT and OCT1) were loaded equally on 4–20% Mini-PROTEAN® TGX™ gels (Bio-Rad) alongside a molecular weight marker (Precision plus protein ...
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bioRxiv - Cancer Biology 2019Quote: ... CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories, Inc.) and imaged on ChemiDoc MP (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: Proteins from WCL samples were separated on 4-20% (w/v) Tris-Glycine SDS-PAGE gels (Bio-Rad; Cat#456-1094, Hercules, CA). We loaded 30-50 μg protein per sample ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Samples were prepared as described in the specific experiment and then loaded on Criterion 4-15% Tris-HCl gels (BioRad 5678083 and 5678084) and separated at 200V ...
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bioRxiv - Neuroscience 2020Quote: ... were mixed with 4× Bio-Rad sample buffer and loaded onto 4%–15% Trisglycine sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA). Proteins were transferred to supported nitrocellulose membrane and probed using Syn101 antibodies against aSyn (BD labs ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Neuroscience 2019Quote: ... were heated at 99 °C for 3 minutes and subjected to 4-20% gradient SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Membranes were then blocked for 1 hour with 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE (90 min at 150V) using 4-20% Criterion™ TGX Stain-Free™ gel (Bio-Rad, 5678095) and electro-blotted onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were recovered from the resulting BAL fluid by centrifugation (500 g, 20 min, 4°C) and counted using an automated cell counter (TC20; Bio-Rad, Hercules, CA). All animal care procedures and experiments were approved by the University of Arizona Animal Care and Use Committee (Approval #13-490).
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bioRxiv - Microbiology 2020Quote: Equal amounts of each rgp120 preparation (10 µg) were separated on a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc.) under denaturing and reducing conditions ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Biophysics 2020Quote: ... placed on a heat block (+90 °C) for 10 min and sonicated before separation by SDS-PAGE (4-20% Mini-PROTEAN TGX Gels, Bio-Rad, 456-1096). Next ...
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bioRxiv - Biophysics 2020Quote: ... Western blot analysis was performed to determine 4-mL fractions with active A2AR collected with a BioFrac fraction collector (BioRad; Hercules, CA, USA), which were then concentrated through a 30-kDa MWCO centrifugal filter (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... the TX-100 soluble fractions were mixed with 5x Tris/Glycerol native sample buffer before loading onto a 4%-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA). The samples were run in Tris/Glycine buffer supplemented with 0.02% Coomassie Brilliant BlueG-250 until the samples had migrated 1-2 cm into the gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Total cellular lysates and amylose pulldown samples were run on a 4-15% gradient SDS-PAGE TGX stain-free gel (Bio-Rad 456-8086) and transferred to an Immobilon-P membrane (Merck Millipore IPVH00010 ...
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bioRxiv - Cancer Biology 2022Quote: ... Eluates or 25-30 μg of protein lysate were loaded in a precast 4-15% gradient SDS-polyacrylamide gel for electrophoresis (mini-protean TGX precast gel, Bio-RAD, # 456-1084).
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... The nascent and total protein samples were then run on a 4-12% gradient SDS-polyacrylamide gel along with Precision Plus Protein Kaleidoscope ladder (Bio-Rad, Cat #1610395). Then the proteins were transferred onto nitrocellulose membranes using either a Bio-Rad Trans-Blot Turbo Transfer system (25V ...
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bioRxiv - Neuroscience 2022Quote: ... size-separated by sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE) in 4-20% Criterion TGX Precast gels and transferred to Trans-Blot Turbo Midi PVDF Transfer Packs (Bio-Rad, Hercules, USA). Membranes were blocked in 5% BSA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... and an equivalent amount of protein lysate (12-15 µg) was electrophoresed on a 4-12% NuPAGE gradient gel and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad, Cat: 1704274). Immunoblotting was performed with primary antibodies followed by secondary antibodies with the indicated dilutions ...
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bioRxiv - Biochemistry 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Size fractioned FBS-free medium from BNT162b1 RNA-transfected HEK293T cells was analysed by denaturing (95° C) and non-denaturating (no-heating) PAGE using 4–15% Criterion™ TGX Stain-Free™ Gel (Bio-Rad) and western blot ...
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
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bioRxiv - Biochemistry 2021Quote: ... of each fraction that eluted within the molecular standard range of 29-669 kDa were run on 4-15% Mini-PROTEAN TGX (Bio-Rad, Hercules, CA) SDS-PAGE gels at 15 V/cm ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels (BioRad, Hercules, CA, Cat#17000435). In addition ...
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bioRxiv - Physiology 2022Quote: ... ran on a 4-20% Criterion TGX Stain-Free Precast Gel and transferred to 0.2 µm PVDF membrane (Bio-Rad Laboratories, Inc, UK). Primary antibodies against FLAG (1:10,000 ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Protein lysates were quantified using BCA assay (Pierce, Waltham, MA) and equivalent amounts were subjected to SDS-PAGE on a 4-15% TGX polyacrylamide gel (Bio-Rad, Hercules, CA). Proteins were transferred onto a polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Purification was performed at 4 °C and protein concentration after the dialysis was determined by DC protein assay (Protein Assay Dye Reagent, Bio-Rad, Hercules, CA) using BSA as standard.
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bioRxiv - Neuroscience 2019Quote: ... were mixed with 4× Bio-Rad sample buffer and loaded onto 4%—15% Trisglycine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: Proteins were separated on 4-20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (Bio-Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualize proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences) ...
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bioRxiv - Biophysics 2019Quote: ... The eluted samples were immediately analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE) using 4–15% precast gradient TGX Stain-FreeTM gels (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2019Quote: ... of the protein was separated by SDS-PAGE in 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (cat. no. 4561084, Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 3 μL of the resuspended membrane pellets containing the receptors were run alongside membrane pellet of uninjected oocyte on 4–15% precast gradient TGX Stain-Free™ gels (Bio-Rad Laboratories) for 34 min at 200 V ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were boiled (95°C) and 18 µL were loaded onto a 4-20% Mini-PROTEAN®TGX™ 12 well-gel (Biorad®). After transfer onto a PVDF (Amersham Hybond ...
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bioRxiv - Cell Biology 2020Quote: Purified recombinant proteins and protein extracts prepared from harvested cells or immunoprecipitation experiments in reducing Laemmli Sample Buffer were run on 4–20 % Mini-PROTEAN® TGX™ Precast Protein Gels of different comb and well-sizes (Bio-Rad, #456-1093 ...