Labshake search
Citations for Bio-Rad :
3251 - 3284 of 3284 citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... 1% Tween-20) before incubation at room temperature for 30 minutes with a Horse Radish Peroxidase (HRP) conjugated secondary antibody (Bio-Rad 1706516 at 1:10,000) in 5% powdered milk (Bio-Rad 170-6404 ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...
-
bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (GE-Healthcare, Chicago, IL, USA) for 1 hour at 15V using the Trans-Blot semi-dry transfer system (Bio-Rad Laboratories, Hercules, CA, USA). After transfer ...
-
A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Both primary antibodies were diluted 1:5,000 in PBST with 0.2% milk and subsequently conjugated with α-rabbit antibody-HRP conjugate (BioRad Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate #1705046 ...
-
bioRxiv - Plant Biology 2021Quote: ... the Agrobacterium-infiltrated leaves were visually inspected at 2-5 days post infiltration and/or the fluorescence of the infiltrated leaves were monitored at 1-2 d post infiltration under the Pro-Q Emerald 488 in the ChemiDoc™ Gel Imaging System (Bio-Rad, Hercules, CA, USA) as described previously (Yoon and Rikkerink 2020).
-
bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 h at room temperature in HRP conjugated IgG anti-rabbit secondary antibody (Bio-Rad 170-65-15, 1:3000) or Cy5 conjugated IgG anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories Europe Ltd. ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 9.5). Quantification of Western blot images (SFig. 1-4) was carried out using ChemiDoc™ XRS+ Image Lab™ Software (Bio-Rad Laboratories AB, Sweden); statistical analysis was performed with GraphPad Prism software (version 5.0 ...
-
bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
-
bioRxiv - Microbiology 2019Quote: ... for 5 min in 2 ml screw-cap vials with 2 g of glass beads (2mm) and 200 μl of buffer containing 1 × TE buffer and Chelex®-100 (Bio-Rad Laboratories, CA, USA). Samples were then incubated overnight at 56 °C with 10 μl of Proteinase K (20mg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
-
bioRxiv - Plant Biology 2022Quote: ... The transfer “sandwich” was exposed to 25 V and 1 A for 30 mins by a Trans-Blot TURBO transfer system (Bio-Rad, catalog no. 170-4155). The membrane was incubated in 4.0% dry milk in PBS buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... and then stained for 1 minute with 0.1% (w/v) Coomassie brilliant blue R-250 (CBBR, Cat#1610400, Bio-Rad Laboratories Inc., Hercules, CA, USA) in methanol/Milli-Q water (1:1) ...
-
bioRxiv - Neuroscience 2022Quote: ... We sampled 1 µl of a cell suspension and quantified the number of living cells by a TC20 automated cell counter (Bio-Rad Laboratories, Hercules, CA USA). Finally ...
-
bioRxiv - Systems Biology 2022Quote: ... shortly centrifuged (2,000xg, 1 min) and the plate heated at 95°C for 60 min in a thermal cycler (Biorad’s S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies used were goat anti-rabbit IgG or anti-mouse IgG (Bio-Rad, 170-6515 or 170-6516, 1:3,000). To prevent signal overlap from IgG heavy chain with eRF1 ...
-
bioRxiv - Bioengineering 2024Quote: ... gene and the HIV-1 Psi vector sequence were performed using respectively custom qPCR HEX assay and custom qPCR FAM assay (Bio-Rad, Marnes-la-Coquette, France) with previously published primer sequences.48 qPCR reactions were performed using 200 ng of gDNA and the Master Mix Taqman Universal II (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were blocked in serum and then incubated in primary antibody for 2 hr at RT (CD-68: 1:500, Bio-Rad clone FA-11, MCA1957GA), and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG ...
-
Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Immunoblots were detected with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:2000; NA934 or NA 931; Amersham BioSciences, Buckinghamshire, UK) by ECL detection (Bio-Rad Laboratories, Hercules, CA, USA). β-Actin served as a loading control.
-
bioRxiv - Biophysics 2024Quote: ... the solution was heated and slowly cooled from 80°C to 4°C (ramp rate of -1°C per 5 s) in a standard thermocycler (Bio-Rad CFX96 Real-Time System) prior to each experiment.
-
bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated overnight at 4°C with antibody cocktail consisting of rat anti-F4/80 (1:100) (catalog #: MCA497; Bio-Rad Laboratories; Hercules, CA, USA), rabbit anti-vimentin (1:100) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 30 seconds and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). PCR products were cleaned using an ExoAP reaction (see Supplemental Methods ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 3:40 min and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). Amplicons were run on a 1% agarose gel and the highest molecular weight fragments (> 5kb ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated in horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature and finally developed using Bio-Rad ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA, cat# 12003154) using ECL substrates (ThermoFisher ...
-
bioRxiv - Microbiology 2023Quote: ... TCA precipitated protein mixtures were dissolved in 30 µL 1 x Laemmli sample buffer and loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Germany). After short 3 cm SDS PAGE separation ...
-
bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
-
bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary horseradish peroxidase-coupled antibodies (1:3000, Pierce, α-mouse #1858413, α-rabbit #1858415) were applied prior to ECL-based chemiluminescence (Biorad #1705061 and ThermoFisher #34094) detected using a Fusion Fx instrument (Vilber Lourmat) ...
-
bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
-
bioRxiv - Microbiology 2021Quote: ... 10 or 1 µl of total lysate and 10 or 1 µg of OMVs were separated on Criterion™ TGX Stain-Free™ any kD™ gel (Bio-Rad, Hercules, California, USA) for Coomassie staining or Western Blot ...
-
bioRxiv - Immunology 2020Quote: ... a total of 6 × 107 cells was incubated in staining buffer with mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:50) at 4°C for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.
-
bioRxiv - Plant Biology 2021Quote: ... Leaf disks were ground to a fine powder evenly with a grinding tool and 120 μl extraction buffer added (30 μl Laemmeli sample buffer 4x [Bio-Rad], 24 μl DTT 1 M, 66 μl dH2O). Tubes were vigorously vortexed for 90 seconds ...