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Citations for Bio-Rad :
3251 - 3300 of 10000+ citations for Formaldehyde Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Lysates from selected wells were further transferred into 96-well RT-PCR plates (Biorad #HSP9641) to perform RT-PCR.
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was used in a reverse transcription reaction with the iScript cDNA Synthesis kit (Bio-Rad, 1708891) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
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bioRxiv - Neuroscience 2023Quote: ... analysis was performed by reverse transcribing 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). iQ quantitative PCR was performed on the diluted cDNA following the manufacturer’s protocol (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: cDNA was synthesized by conversion of 1 µg of the DNAse-I treated RNA with iScript Selected cDNA Synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the Reverse Transcription system (iScriptTM cDNA synthesis kit, Bio-Rad). Quantitative Real time PCR was performed using Sybr Green Master Mix (SYBR Green master mix ...
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bioRxiv - Systems Biology 2023Quote: We performed cDNA synthesis with 0.5-1 μg of total purified RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... diluted to 1 ng/ μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were treated with ECL chemiluminescence detection reagent (GE healthcare, product # RPN2106V1 and RPN2106V20 and imaged using the Bio-Rad Chemidoc system (Bio-Rad Laboratories). Densitometry was done using ImageLab software (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... and primers and probes as previously described [21] in a CFX 96™ real-time PCR thermocycler and detection system (BIO-Rad, Marnes-la-Coquette, France). To estimate M ...
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bioRxiv - Biophysics 2021Quote: ... The level of virus mRNA was quantified using SYBR Premix Ex Tag II (Tli RnaseH Plus) on CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA), the reaction system is the same as before ...
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bioRxiv - Molecular Biology 2020Quote: ... The duplex and triplex TaqMan® qPCRs were also carried out in the Bio-Rad CFX 96 Real-Time Detection System (Hercules, Bio-Rad Laboratories GmbH, Munich, Germany), using the same thermal program as described above ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Real-time quantitative PCR using SYBR green chemistry was performed using the i-Cycler iQ Real-time Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Samples were amplified in triplicate in 96-well optical plates (Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using iQ™ SYBR®Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad, CA, USA). All primers were obtained from Sigma-Aldrich and described in Suppl ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were reverse transcribed and amplified using TaqMan™ chemistry and the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). Primers and probe targeted the glycoprotein gene from EBOV (GenBank accession no ...
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bioRxiv - Plant Biology 2022Quote: ... 20 pmol of each primer in a total reaction volume of 10 μL on a C1000™ Thermal Cycler with a CFX384™ Touch Realm-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) with the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... The raw qPCR data were obtained by the CFX Maestro™ Software for CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories, CA, USA). mRNA levels of genes of interest were normalized to the geometric mean of expression levels of two reference genes Actb and Gapdh ...
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bioRxiv - Physiology 2023Quote: ... Quantitative RT-PCR was performed using Hieff® qPCR SYBR® Green Master Mix (No Rox) (Yeasen biotech Co., Ltd., Shanghai) on a CFX96 Real-Time PCR Detection System (Bio-Rad, Los Angeles, CA, USA). β-tubulin was used as a housekeeping reference gene ...
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...