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Citations for Bio-Rad :
3251 - 3300 of 7983 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated on a 4–20% gradient Mini-Protean TGX Gel Stain-free (Bio-Rad, Hercules, CA, USA). The separated proteins were blotted on nitrocellulose membranes using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: 40-50 µg of cleared cell lysates were separated on 4-20% gradient gels (Mini-Protean or Criterion TGX, Bio-Rad) by SDS-PAGE and transferred to nitrocellulose for immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: ... and equal amounts of protein were prepared to SDS-Page using a 4-20% gradient polyacrylamide gel (Bio-Rad, Hercules, CA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: ... About 100 μg of protein with 2x serial dilutions were loaded onto 4–20% TGX™ precast mini gels (BioRad, England) either with or without reduction with 50 mM dithiothreitol (DTT) ...
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bioRxiv - Biochemistry 2024Quote: ... 20% (v/v) glycerol and 0.01% (w/v) bromophenol blue) and loaded onto Mini-PROTEAN TGX™ 4–20% precast gels (Bio-Rad). As a reference ...
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bioRxiv - Cell Biology 2024Quote: Samples then were subjected to SDS-PAGE using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad, 5678084), then transferred onto a PVDF membrane (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Samples were combined with Laemmli Sample buffer + 10% BME and separated on pre-cast 4-20% SDS- PAGE gels (Bio-Rad). Gels were transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were analyzed on either a 12% Bis Tris PAGE gel (for histone analytes) or a 4-12% Bis Tris PAGE gel (for all other analytes) using 1x MES running buffer (Bio-Rad) at 180 V ...
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bioRxiv - Cancer Biology 2024Quote: Western blotting was performed using 10 µg protein lysate (25 µg for detecting DARPins) mixed with 4 x Laemmli sample buffer (Bio-Rad) and diluted to 1 x with dH2O and addition of β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-polyacrylamide gel electrophoresis (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, BIO-RAD), transferred to nitrocellulose ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein lysates were centrifuged (20,000 g, 20 min, 4°C) and protein concentrations were obtained using Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were resolved by SDS-PAGE on 4-15% gradient Mini-PROTEAN® TGX precast protein gel (Bio-Rad, cat. 4561086) in 1x Tris/glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Resultant RNA-Protein (RNP) complexes were resolved by native polyacrylamide gel electrophoresis (4-15% Tris-Glycine gradient gels, Bio-Rad #5671084) at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
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bioRxiv - Neuroscience 2024Quote: ... was carried out with 5 ng of cDNA in a volume of 4 μl with 1 μl of 5 μM forward and reverse primers mix and 5 μl of SsoFast EvaGreen Supermix premix (Biorad, CN172-5204). Triplicate reactions were carried out for each mRNA ...
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bioRxiv - Cell Biology 2020Quote: ... X-ray film (Figs 1-5) and the ChemiDoc MP Imaging System (Bio-Rad, cat#17001402) (Supplemental Fig 3).
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bioRxiv - Microbiology 2021Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... and applied to a 5 mL prepacked Mini ceramic hydroxyapatite (CHT) type 1 column (Bio-Rad). The flowthrough was collected and purified by SEC using a HiLoad 16/600 Superdex 200 (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with Tidyblot (diluted 1:5000 in 5% BSA in TBST, Biorad, USA), for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-cPLA2, 1:100, Cell Signalling; mouse anti-LaminA/C, 1:500, ABCAM; rat anti-tyrosinated α-tubulin 1:500, Bio-Rad; mouse γ-H2AX, 1:2000) in blocking solution for 1h at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... and mouse hypoxanthine-guanine phosphoribosyltransferase gene (Hprt) (forward, 5’-GGACTTGAATCAAGTTTGTG-3’; reverse, 5’-CAGATGTTTCCAAACTCAAC-3’) in a CFX96 Real-Time PCR Detection System (Bio-Rad). The qRT-PCR results were analyzed using the ΔCt method (relative expression ...
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bioRxiv - Microbiology 2023Quote: ... US28 (sense, 5’-CCAGAATCGTTGCGGTGTCTCAGT-3’; antisense, 5’-CGTGTCCACAAACAGCGTCAGGT-3’) and GAPDH were detected with CFX Connect Real Time System (Bio-Rad) using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... and primer set previously described [25] CtPl+ 5’-TAGTAACTGCCACTTCATCA-3’ and CtP2 5’-TTCCCCTTGTAATTCGTTGC-3’) and using a CFX96TM real-time thermocycler (Bio-Rad)) ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres (1000/well) were incubated with serially diluted samples (1:100, 1:1000, 1:10,000) in replicates in Bioplex plates (Bio-Rad) at 4°C for 16hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was diluted 1:1 with 2X Laemmli sample buffer (BioRad, 1610737) under reducing conditions and boiled for 5 minutes ...
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bioRxiv - Physiology 2021Quote: ... Samples were mixed 1:1 with Laemmli’s sample buffer (BioRad 161-0737) containing 10% 2-Mercaptoethanol (Sigma M3148 ...
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bioRxiv - Immunology 2022Quote: ... samples were mixed 1:1 with 2 x native sample buffer (BioRad) and loaded on a 4-20% precast protein gel (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Rat anti-F4/80 (Bio-Rad, clone CI:A3-1, #MCA497GA, 1:50), Rabbit anti-CD45 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.4 μM final concentrations of the gene-specific PCR primers on a CFX Connect Real-Time System thermal cycler (Biorad, Hercules, California). The qPCR conditions were 95°C for 3’ ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Physiology 2020Quote: ... tissue biopsies were transferred into the assay medium for a 4h incubation (pre-incubation medium supplemented with 4 mg/ml glucose and 4.3% Alamar Blue solution (Bio-rad, Hercules, CA)) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples collected before and after UV-crosslinking were subsequently analysed by SDS-PAGE in 4-20% gradient gels (Bio-Rad TGX). Gels were subsequently transferred onto a PVDF membrane and FLAG epitopes were detected by Sigma M2 antibodies followed by ECL imaging.
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... Samples were diluted four-fold with water before being loaded onto a 4-20% tris-glycine polyacrylamide gel (BioRad, Hercules, CA, USA), approximately ∼10µg of protein (i.e. ...