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Citations for Bio-Rad :
3201 - 3250 of 4210 citations for Cow Porphobilinogen deaminase HMBS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Lysate was spun down at 14000 rpm and protein concentration was determined using the Bradford assay as detailed in the manufacturer’s kit (Biorad). Samples were denatured with SDS and methylene blue ...
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bioRxiv - Cell Biology 2021Quote: ... and total cellular protein content was determined using the DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). Then ...
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bioRxiv - Genomics 2021Quote: ... Protein concentrations of the lysates were determined using a Bio-Rad DC protein assay kit (Bio-Rad, catalog # 5000111), the lysates were denatured in the SDS-PAGE loading buffer at 100°C for 10 min ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... 500 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit according to manufacturer instructions (BioRad). Primers used for RT-qPCR are shown in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of RNA were converted into cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA; 1708891) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed to cDNA using the iScript™ cDNA Synthesis kit (Bio-Rad #1708890, Hercules, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from total RNA via reverse transcription reaction using an iScript RNA-to-cDNA kit according to the manufacturer’s instructions (BioRad). cDNA was then amplified using the iQ SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... RNA templates were then utilized for complimentary DNA (cDNA) synthesis utilizing the iScript cDNA synthesis kit protocol (Bio-Rad).
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of purified RNA was reverse transcribed using either the iScript gDNA Clear cDNA Synthesis Kit (BioRad, #1725034) or Maxima H Minus First Stand cDNA Synthesis Kit (Thermo Fisher Scientific M1681) ...
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bioRxiv - Physiology 2022Quote: ... and the first-strand cDNA was prepared using the iScriptTM cDNA synthesis kit (Bio-Rad, #1708890, Hercules, CA, USA). qPCR analysis was performed with SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants were used for protein quantification via the use of the DC(tm) Protein Assay Kit (Bio-Rad). Equal loading of proteins (10–20 µg ...
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bioRxiv - Plant Biology 2022Quote: One microgram of RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative real-time PCR analysis was performed on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... One-step RT-qPCR was performed using an iTaq Universal SYBR Green One-Step RT-qPCR Kit (BIO-RAD). The reaction was performed in a total volume of 20 μL by mixing 10 μL iTaq universal SYBR Green reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was analyzed by TaqMan qRT-PCR one-step RT-PCR kit for probes (Bio-Rad laboratories, Italy) with a TaqMan RT-PCR instrument (CFX384 real time system ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Microbiology 2023Quote: ... was used to purify RNA followed by cDNA synthesis (0.5 μg of RNA) using iScript RT kit (Bio-Rad) and qPCR was performed using the Brilliant II SYBR® Green QPCR master mix (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were blotted onto a nitrocellulose membrane using the Trans-Blot Turbo TRA Transfer Kit (Bio-Rad, Feldkirchen, Germany). Following protein transfer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunoblot was performed by transferring protein to a PVDF membrane using Trans-Blot Turbo RTA Transfer Kit (Biorad, 1704272) and the Biorad Transblot Turbo System ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was then incubated in a transfer buffer from the trans-blot turbo RTA transfer kit (Biorad, 1704272) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... at 100 V and then transferred using the Trans-Blot Turbo transfer kit onto nitrocellulose membranes (Bio-Rad, #1704270). Membranes were blocked with 5% nonfat dry milk prepared in TBST (Tris-buffered saline with Tween 20 ...
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bioRxiv - Immunology 2023Quote: ... and 500 ng of total RNA was reverse transcribed using the iScript™ gDNA Clear cDNA Synthesis Kit (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized using oligo(dT)/random primer mediated reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad) with 1 μg of DNA-free total RNA as input ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was purified from dissected tissues using an Aurum Total RNA Fatty and Fibrous Tissue kit (Biorad 7326830) and then quantified with a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng - 1μg DNase-treated RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instruction and the resulting cDNA was used for reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2022Quote: ... and a calibration curve of protein content was created from the BSA protein standard kit (Bio-Rad Laboratories, CA). An equal amount of protein (50 ng ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis for qRT-PCR was done with iScript® gDNA Clear cDNA Synthesis kit (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Synthesis of cDNA from 100 ng input RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Primers were pre-designed (PrimeTime qPCR Primers ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were lysed in 350 μl/well lysis buffer from the AurumTM total RNA Mini Kit (Biorad, 7326820). The lysates were immediately frozen at −80 °C until RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... Only samples with an RNA integrity score (RIN) greater than 8.0 were selected for the preparation of cDNA libraries using iScript cDNA Synthesis Kit (BioRad). One nanogram of total RNA was amplified to several micrograms of cDNA.
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA was synthesized from 500 ng of total RNA with the iScript cDNA synthesis kit (Bio-Rad) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were separated by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-PROTEAN kit (1658001FC, Bio-Rad). Between 20 and 50 μg of protein per well were loaded ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transcribed into cDNA using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, Hercules, CA). Real time PCR (Polymerase Chain Reaction ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA titers and infections were assessed via qRT-PCR using an iTaq Universal Probes One-Step Kit (BioRad) in a 20 µl sample volume ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of the purified RNA was employed to synthesize cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). RT-qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... using 11 μL undiluted RNA and the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Cat# 1864021). Droplets were generated ...
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bioRxiv - Cell Biology 2023Quote: ... following the procedure of Stoeckius et al.[28] using the LYNX Rapid Streptavidin Antibody Conjugation Kit (Biorad, Ref #LNK163STR). Briefly ...
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bioRxiv - Pathology 2024Quote: ... First strand cDNA was synthesized from 500 ng RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Real-time PCR was carried out using TaqMan assays in a 10 μl reaction mixture (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... according to manufacturer’s protocol and used for reverse transcription with iScript cDNA synthesis kit (Bio-rad, cat #170-8890) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Microbiology 2023Quote: ... NS1 antigen levels were measured using the Platelia™ Dengue NS1 Antigen kit (Cat No. 72830, Bio-Rad, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNA was isolated with TRIzol RNA Isolation protocol and used to make cDNA using iScriptTM cDNA Synthesis Kit (BioRad). The StepOneTM Real-Time PCR System was used to analyze the samples under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 μg total RNA was reverse transcribed to generate cDNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions ...