Labshake search
Citations for Bio-Rad :
3201 - 3250 of 4080 citations for 7 Trifluoromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
-
bioRxiv - Plant Biology 2024Quote: ... The gels were visualised using a UV trans-illuminator Gel Doc 2000 and Quantity One 1-D analysis v4.6.5 software (Bio-Rad).
-
bioRxiv - Immunology 2023Quote: ... HSV-1 DNA was quantified by TaqMan qPCR on a CFX96 Touch real-time PCR detection system (Bio-Rad) against a standard curve generated by extracting DNA from an HSV-1 viral stock ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
-
bioRxiv - Microbiology 2024Quote: ... Cell lysates and purified supernatants were separated on an SDS-PAGE and analyzed through immunoblotting against vinculin as a loading control (1:2,000, MGA465GA, BioRad) or CHIKV E1 glycoprotein (1:1,000 ...
-
bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
-
bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was performed on 1 µg RNA with the iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) for 5 min at 25°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
-
bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
-
bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
-
bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used for immunofluorescence at the indicated concentrations: α-Tubulin (Bio-rad, MCA77G, 1:500), β-Tubulin (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... each primary was detected via HRP conjugated Goat α-Rabbit secondary antibody (1:4000, Bio-Rad, product 170-6515) after incubation with Clarity ECL substrate (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit (Cat. # 170-6515) antibodies were used at 1:2000 for immunoblotting and purchased from Biorad.
-
bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma ...
-
bioRxiv - Immunology 2024Quote: ... qPCR was then performed with a 1:10 dilution of cDNA using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 6 (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... Amplicons were resolved in a 1% agarose gel and visualized using the ChemiDoc MP Imaging System (Biorad, Hercules, CA). When specified ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
-
bioRxiv - Cell Biology 2024Quote: Polyacrylamide gel electrophoresis was carried out using 10 % gels (acrylamide/bis-acrylamide ratio 37.5:1) or any kDa precast gels (Biorad) and a MiniProtean (BioRad ...
-
bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from 1 µg total input RNA using iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601) ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were washed with TBST and incubated with StarBright Blue 700 Goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 hour ...
-
bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
-
bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were the following: HRP-conjugated goat anti-mouse IgG (1:10000, Bio-Rad, 170-6516); HRP-conjugated goat anti-rabbit IgG (1:10000 ...
-
bioRxiv - Microbiology 2023Quote: ... in 1×TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo Transfer System (BioRad). Blocking was performed in a 5% solution of dry milk and 0.05% Tween 1×PBS (1×PBST ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Axonal staining and myelination in the SLM were assessed with rat anti MBP (BIO-RAD, cat # MCA409S 1:500) and mouse anti-Neurofilament H (NF-H) ...
-
bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
-
bioRxiv - Physiology 2023Quote: After washing with TBS-T 0.1% membranes were incubated with goat anti-mouse (1:1000, Bio-Rad, Madrid, Spain), goat anti-rabbit (1:1000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant was diluted 1 in 10 and 4µl of diluted supernatant was added to 200µl of Bradford reagent (Bio-Rad) at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
-
bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
-
bioRxiv - Genomics 2023Quote: ... For quantitative real-time PCR 20 ng cDNA and 1 pmol/μl primers were mixed with 2x SYBR (BioRad) in a total volume of 13 μl for each well ...
-
bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
-
bioRxiv - Biochemistry 2023Quote: ... CCT was immunoprecipitated from the lysate by adding 4 µg of a CCT5 antibody (BioRAD MCA2178, 1:100 IP) and incubating for 30 minutes at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... and total RNA (1 μg) was reverse transcribed to cDNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, Mississauga, Canada). SYBR Green (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... secondary antibodies (IgG(H+L)-HRP) were used at 1:10000 dilution for detection using a Versadoc system (Biorad).
-
bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
-
bioRxiv - Biophysics 2023Quote: ... 50 mM Tris pH 7.4 was added to quench the reaction and the samples were buffer exchanged into BLI buffer (20mM Tris pH 7.4, 100mM NaCl, 1% glycerol, and 0.03% DDM) using PD-10 columns (Bio-Rad). For enterotoxins ...
-
bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
-
bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
-
bioRxiv - Microbiology 2023Quote: ... diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). Relative expression was calculated by ΔΔCt method to GapDH 66 ...
-
bioRxiv - Cell Biology 2023Quote: ... Thawed lysates were run on 7.5% Acrylamide gels with a 29:1 ratio Acrylamide:Bis using a Protean Tetra system (Bio-Rad) and Precision Plus markers (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...