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Citations for Bio-Rad :
3151 - 3200 of 7469 citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Resultant RNA-Protein (RNP) complexes were resolved by native polyacrylamide gel electrophoresis (4-15% Tris-Glycine gradient gels, Bio-Rad #5671084) at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... and 3 μL of N,N,N°,N°-tetramethyl ethylenediamine (Bio-Rad) were added to initiate the acrylamide polymerization ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.4 μM final concentrations of the gene-specific PCR primers on a CFX Connect Real-Time System thermal cycler (Biorad, Hercules, California). The qPCR conditions were 95°C for 3’ ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Genetics 2021Quote: ... Forty micrograms of total protein from hippocampal nuclear lysates was boiled for 5-minutes to dissociate complexes before separation in a 4-20% gradient gel (Bio-Rad 4561096) for 1-hour at 150V in 1x Tris/Glycine/SDS (Bio-Rad 1610732) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Physiology 2020Quote: ... tissue biopsies were transferred into the assay medium for a 4h incubation (pre-incubation medium supplemented with 4 mg/ml glucose and 4.3% Alamar Blue solution (Bio-rad, Hercules, CA)) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples collected before and after UV-crosslinking were subsequently analysed by SDS-PAGE in 4-20% gradient gels (Bio-Rad TGX). Gels were subsequently transferred onto a PVDF membrane and FLAG epitopes were detected by Sigma M2 antibodies followed by ECL imaging.
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... Samples were diluted four-fold with water before being loaded onto a 4-20% tris-glycine polyacrylamide gel (BioRad, Hercules, CA, USA), approximately ∼10µg of protein (i.e. ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples prepared in 4X Laemmli buffer were run on a 4-20% Mini-protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA) and transferred to nitrocellulose using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... 20μg of protein were loaded into each lane of a Criterion™ XT Bis-Tris Precast Gel 4-12% (Bio-Rad, UK) for SDS-Page separation ...
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bioRxiv - Plant Biology 2020Quote: ... The mixture was kept for 5 min at 95°C and 5 min on ice before loading on a TGX 4-20% Strainfree (Bio-Rad US) gel immersed in TGS 1X buffer ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad) and visualized with GelCode Blue stain (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... A total of 25 μg of protein was loaded onto a 4–20% Mini-PROTEAN® TGX™ Precast Gel (Bio-Rad) and transferred onto a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... samples were transferred directly to ice-cold A4P0 solution consisting of: 4% acrylamide monomer (40% acrylamide solution, cat. 161-0140, Bio-Rad, UK), 0.25% VA-044 (thermoinitiator ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA in a 20 μl reaction with 4 μl iScript reverse transcription supermix (Bio-Rad #1708841) at 25 °C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... a part of each sample was run on a 4-15% Criterion TGX Stain-Free Protein Gel (26 wells, Bio-Rad, #5678085) in 1x SDS running buffer at 200 V for 32 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were loaded on 10% or 4-15% sodium dodecyl sulfate polyacrylamide gels (Fast Cast TGX Stain-Free or 4-15% Mini-Protean TGX Stain-Free gels, BioRad, Hercules, CA). Blotting was done on PVDF membranes using a BioRad Transblot system ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated using SDS-PAGE on 4-15% gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 4°C for 15 minutes at 16,000 g and protein concentration was measured by the Bradford method (Bio-Rad, Hercules, USA). Next ...
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bioRxiv - Cell Biology 2021Quote: ... Purified septin complexes were analyzed by SDS-PAGE using 4-20% precast polyacrylamide gels (Mini-PROTEAN TGX Gels from Bio-Rad, 4561095), or hand-casted 12% or 15% Tris-glycine polyacrylamide gels ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were loaded on 4-12% Bis-Tris gel and separated by SDS-PAGE on the Criterion system (Bio-Rad Laboratories), and electrophoretically transferred to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: 15 µg of nuclear extract in SDS loading buffer or 30 µg of total protein extract were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE of the re-oxidized samples was performed on a 4-15% Criterion TGX precast midi protein gel (Bio-Rad, 5671085) under non-reducing conditions ...
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bioRxiv - Cell Biology 2022Quote: ... equal amounts of lysate were loaded into wells of 12% Mini-PROTEAN® TGX 4-20% pre-cast protein gel (BIO-RAD) installed in Mini-PROTEAN® tetra vertical electrophoresis cell filled with 1X TG-SDS solution (Euromedex) ...
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bioRxiv - Microbiology 2021Quote: ... Equal total protein was loaded from each of the indicated cell lysates and run on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad). Protein was transferred at 4°C to nitrocellulose membrane for 2 hours at 50V ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then boiled at 95°C for 10 mins and resolved in 4-15 % Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) for immunoblot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract was loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Biorad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... Samples were electrophoretically resolved on gelatin-containing gels at 125 V for 110 min at 4 °C using a Power Pack supply (Bio-Rad, USA) Next ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn). Non-His-Tagged proteins were eluted with lysis buffer until the absorbance at 280 nm matched background levels utilizing 50 – 100 mL of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg/lane of S was run on 4–20% Mini- PROTEAN® TGX Stain-Free™ Protein Gels (BioRad, Cat#4568081), transferred to nitrocellulose (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal protein (20ug) content were combined with loading buffer and separated using standard gel electrophoresis on 4-20% polyacryllamide gel (Bio-Rad Techonolgies) and then partial-wet transferred to nitrocellulose membranes (BioRad) ...