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Citations for Bio-Rad :
3101 - 3150 of 4305 citations for 6 4 Methoxybenzylcarbamoyl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-30μg protein samples were loaded into 4-20% Mini-PROTEIN TGX Precast Protein Gels (Bio-Rad, Cat# 4561096), and then were transferred to 0.2µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled before being subjected to SDS-PAGE on Mini-PROTEAN TGX Precast Gel 4-15 % (Biorad, 4561086). Western blot using anti-SUMO antibody (non-commercial ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled before being subjected to SDS-PAGE on Mini-PROTEAN TGX Precast Gel 4-15 % (Biorad, 4561086). Western blot using anti-GFP (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of each sample was loaded onto a 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 180 V for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Samples were diluted to 4 M urea with water for Bradford Assay quantification (Bio-Rad Laboratories, Inc, Hercules, CA) and trypsin digestion ...
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bioRxiv - Microbiology 2024Quote: Protein fractions were analyzed by standard denaturing SDS-PAGE electrophoresis using 4 to 20% gradient mini-gels (Bio-Rad), InstantBlue Coomassie protein stain ...
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bioRxiv - Biophysics 2024Quote: ... Equal volumes of protein samples were resolved on 4-12% gradient SDS-polyacrylamide gel and transferred onto 0.2μM nitrocellulose (BioRad #1620112) or PVDF (BioRad #1620177 ...
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bioRxiv - Neuroscience 2024Quote: ... Each sample (15 μg) was boiled for 5 min and applied on NuPAGE 4%– 12% Bis-Tris Gel (Biorad). The gel was transferred onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... and the samples were then boiled in the Laemmli buffer and loaded into 4%–20% gradient gel (Bio-Rad), transferred to PVDF membrane (Millipore) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on a 4 to 20% Trist-glycine stain free polyacrylamide 10-well gel (Bio-Rad) at 170 V for approximately 1 h in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084), and the gel was stained using QC Colloidal Coomassie Stain (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 40 µg of worm extract were loaded per well onto a precast 4-20%TGX gel (Bio-Rad, #4561094), and run in TGS buffer at 200 V for 45 min.
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated for 5 minutes (tissue ≤ 40μm) or 10 minutes (tissue ≥ 100μm) in polyacrylamide (PA) solution [4% acrylamide/bis acrylamide (BioRad., 1610154), 60mM Tris HCl pH 8 (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were analyzed with SDS–PAGE using 4-15% Mini-PROTEAN TGX Stain-Free Gels (4568083 Bio-Rad). Proteins were transferred to a nitrocellulose membrane (1704271 ...
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bioRxiv - Microbiology 2024Quote: ... The protein lysates were centrifuged and the supernatants were subjected to SDS-PAGE (gradient, 4 to 20%; Bio-Rad) and electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by electrophoresis using 4-12% Bis-Tris mini protein gels and transferred to PVDF membranes (BioRad). Blots were incubated with primary antibodies at 4°C and then with HRP conjugated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were loaded and run on 4-20% Criterion TGX Midi Protean gels (Bio-Rad #5671094, Hercules, CA, USA) and transferred to nitrocellulose 0.45 µm membrane (Bio-Rad #1620113 ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The same amount of protein was loaded in each lane of Mini-PROTEAN TGX gels (4–20%, Bio-Rad). The antibody signals were detected by chemiluminescence using ImmunoStar LD reagent (290-69904 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated by SDS–PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate were separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BIO-RAD), transferred to polyvinylidene difluoride membranes (0.2 um ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Microbiology 2020Quote: ... Two microgram of concentrated viruses were resolved on 4-20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Biorad) using the Novex™ Sharp Pre-stained Protein Standard (Thermo Fisher Scientific ...