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Citations for Bio-Rad :
3051 - 3100 of 4472 citations for Rat Tsukushin Tsku ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... total RNA was converted to complementary DNA (cDNA) using iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). The cDNA obtained was subjected to qRT-PCR using the itaq™ Universal SYBR green kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 300 ng of RNA using the iScript Select cDNA Synthesis kit (Bio-Rad). Gene expression analysis was done by qPCR using cDNA (300-fold dilution ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated using 500 ng of total RNA with the iSynthesis cDNA kit (Bio-Rad #1708891). RT-qPCR was performed on a CFX Connect Real-Time Detection System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... 16uL of RNA isolated from fractions was reverse transcribed (iScript cDNA synthesis kit, Bio-Rad cat# 1708840) followed by qPCR (iTaq SYBRgreen Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... The Luminex kits EGFR Y1068-p and p-AKT is S473-p were obtained from Bio-Rad. The capture AXL antibody was generated by conjugating a primary AXL antibody (R&D systems MAB154 ...
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bioRxiv - Cell Biology 2023Quote: ... After the protein concentration was measured via Lowry assay using DCTM Protein Assay Kit (BIO-RAD, 5000111), the same amount of total protein was used for each pulldown (2-3 mg per pulldown) ...
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bioRxiv - Bioengineering 2023Quote: ... protein transfer was performed viaTrans-Blot® Turbo™ RTA Mini PVDF Transfer Kit (Bio-Rad, USA). The membrane was blocked with PBS-T (0.1% Tween-20 ...
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used for cDNA synthesis with iScript cDNA Synthesis Kits (BioRad, Hercules, CA) in a 20μl reaction volume ...
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bioRxiv - Cancer Biology 2024Quote: ... First strand cDNA was generated from isolated RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708890) and RT-qPCR performed on the resulting cDNA (diluted 5-fold ...
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bioRxiv - Molecular Biology 2024Quote: Protein estimation was performed in cell lysates by BCA protein assay kit (Bio-Rad, Hercules, CA, USA). For Western blots ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1 µg RNA samples using the iScript cDNA Synthesis kit (Bio-Rad, USA). qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was carried out using iTaq Universal SYBR Green One-Step Kit (Bio-Rad, #1725150). 50 ng of RNA was mixed with primers for targeted genes ...
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bioRxiv - Plant Biology 2024Quote: ... then reverse-transcribed using iScriptTM cDNA synthesis kit (cat. nr. 1708891) (Bio-Rad Laboratories, Hercules, CA, USA) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined according to the Bio-Rad Protein Assay Kit II (Bio-Rad cat. 5000006). Once quantified ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by reverse transcription and cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad). The cDNA was then used for qPCR analysis with iTaq™ Universal SYBR®Green Supermix (Bio-rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred onto nitrocellulose membranes using Trans-Blot® TurboTM Transfer System RTA Transfer Kit (BioRad). Blotted membranes were blocked in 10% non-fat milk in PBS-Tween 20 0.05% for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Reverse transcription into cDNA was performed according to iScript manufacturer instructions (iScript cDNA synthesis kit, Biorad 1708891). Gene expression was measured by Taqman qPCR on the cDNA using 20 μl reactions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR was performed using the PerfectStart Green qPCR SuperMix kit (TransGen Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... One μg RNA was converted to cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA) and 1:10 dilution of cDNA was used for each qPCR reaction ...
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bioRxiv - Genomics 2024Quote: Extracted viral (and control) RNAs were reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad, 1708891), per manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II, Cat# 5000112, Biorad). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Pathology 2024Quote: ... starting with reverse transcription into cDNA using the iScript Reverse Transcription kit (Bio-Rad, 1708841Hercules, California, USA). The primers for all genes can be seen in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... substrate kit and X-ray (Agfa, Mortsel, Belgium) films or ChemiDoc™ Touch Imaging System (Bio-rad). For dot-blot analysis ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from ∼ 1 μg of extracted RNA using an iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed for IFNB and IL-1β on the Applied Biosystems PRISM 7500 qPCR machine ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). The cDNA was diluted 1:5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used for reverse transcription using an iScript cDNA synthesis kit (Bio-Rad). cDNA was diluted 5 times in Milli-Q water and used as a template for PCR amplification using GoTaq G2 Green Master Mix (Promega ...
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bioRxiv - Physiology 2024Quote: ... Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II, Cat# 5000112, Biorad). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... total protein levels were determined using a DC protein assay kit (Bio-Rad Laboratories GmbH, Munich, Germany), and equal protein concentrations were adjusted ...
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bioRxiv - Cell Biology 2024Quote: ... complementary DNA (cDNA) was synthesized from the RNA using the iScript cDNA Synthesis kit (BioRad, #170-8891). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Pathology 2024Quote: ... RNA extracts were treated with DNAse and reversed transcribed using gDNA Clear cDNA Synthesis kit (Bio-Rad). qPCR was conducted in 10 µl final volume with 2 μl of 5X HOT Pol EvaGreen qPCR mix plus (Euromedex) ...
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bioRxiv - Pathology 2024Quote: ... RNA extracts were treated with DNAse and reversed transcribed using gDNA Clear cDNA Synthesis kit (Bio-Rad). qPCR was conducted in total reaction volume of 10 µl containing 2 μl of cDNA ...
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bioRxiv - Genetics 2024Quote: ... 400 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad, 1708891). The cDNA region containing phased heterozygous SNP of ANXA11 – rs2573353 in WTC11141 – was amplified using the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were developed using the ECL kit and scanned with ChemiDoc MP Imaging system (Bio-Rad). The blots were quantified with Image Studio Lite software and GraphPad Prism were used for statistical analysis (unpaired t test ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1mM EDTA) and protein concentration was measured using the DC protein assay kit (Bio-Rad,5000112) and absorbance was read on the Synergy H1 ...
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bioRxiv - Cell Biology 2024Quote: SDS-PAGE analysis was carried out either with 10% gels (TGX FastCast acrylamide Kit 10%; Bio-Rad) or precast 4-15% gradient gels (Mini-PROTEAN TGX precast gel ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of total RNA were used for cDNA synthesis using an iScript cDNA Synthesis kit (Bio-Rad). Quantitative analysis of RPS6KA2 was performed using a SsoAdvanced™ Universal SYBR® Green Supermix (Biorad ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Cancer Biology 2021Quote: ... One microgram of RNA was reverse-transcribed to cDNA using the BioRad 5X iScript RT supermix kit (BioRad Laboratories Canada ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with the Quick Start™ Bradford Protein Assay kit (Bio-Rad Laboratories, Hercules, CA) using a concentration series of bovine serum albumin solution as standards.
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were cleared with centrifugation and protein concentrations were measured with Protein Assay kit (Bio-Rad, #5000001). 25-50 μg protein were loaded to 10-well SDS-PAGE gradient gel (Bio-Rad 456-1094) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... as well an Experion™ bioanalyser with a prokaryotic RNA standard sensitivity analysis kit (Bio-Rad, California, USA). One μg of RNA from each of the propleural plate samples was sent to Vertis Biotechnologie AG (Freising-Weihenstephan ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and protein concentration quantified using a Bio-Rad protein assay kit (BioRad Laboratories, USA) and later stored at -80°C until use.
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and then amplified by real-time quantitative polymerase-chain reaction (RT-qPCR, SybrGreen PCR MasterMix Kit, Bio-Rad) using primers listed in Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... the RNA was reverse transcribed to cDNA (Revertid First Strand cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) and then amplified by real-time quantitative polymerase-chain reaction (RT-qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared with 1000ng of DnaseI treated RNA (iScript Select cDNA synthesis Kit, BioRad, following manufacturer’s instructions). Random hexamers were used for the nascent RNA experiment showed in Figure 2F and Figure 2 - figure supplement 2A ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was used to make cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). All primer pairs were designed and validated in-house for efficiency and specificity ...