Labshake search
Citations for Bio-Rad :
3051 - 3100 of 4474 citations for Human Cathepsin B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... and then transferred to nitrocellulose membranes using the Bio-Rad TurboTransfer system and kits (Bio-Rad #1704270). Membranes were blocked with 5% milk in Tris-buffered saline and 0.1% Tween 20 (TBST ...
-
bioRxiv - Immunology 2023Quote: ... DNase treatment and cDNA synthesis was performed with the iScriptTM gDNA Clear cDNA Synthesis kit (BioRad, #1725035) following the manufacture’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... 600ng of total RNA was converted to cDNA using the iScript Select cDNA Synthesis kit (Bio-Rad). Quantitative RT-PCR was performed in triplicates using SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis was performed using 1µg of total RNA and the iScript Reverse Transcription Kit (BIO-RAD) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... The corresponding cDNAs of the samples were prepared using the iScript Select cDNA synthesis kit (Biorad, 1708891). Primers for qPCRs were used at a final concentration of 0.2 µM ...
-
bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein concentrations from tissue lysates were determined using the Bradford assay kit (Biorad, USA; Cat# 5000006). Equal amounts of protein (30 μg ...
-
bioRxiv - Plant Biology 2023Quote: ... and 500 ng of total RNA was used to synthesize cDNA using iScript reverse transcription kit (Biorad). The cDNA sample was diluted 20 times with nuclease-free water ...
-
bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used for cDNA synthesis with iScript cDNA Synthesis Kits (BioRad, Hercules, CA) in a 20μl reaction volume ...
-
bioRxiv - Microbiology 2024Quote: ... The protein concentration in the cell lysates was determined using a DCTM protein assay kit (BioRad, 5000111). The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad ...
-
bioRxiv - Zoology 2024Quote: ... cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories), and reactions were assembled with sSoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Soluble proteins were quantified by Lowry’s method using the DC Protein Assay Kit (Biorad, Hercules, CA, USA). Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad ...
-
bioRxiv - Physiology 2024Quote: ... The protein concentration of the lysate was measured using a DC Protein Assay Kit (Bio-Rad; 5000111). Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 300 ng of RNA using the iScript Select cDNA Synthesis kit (Bio-Rad). Gene expression analysis was done by qPCR using cDNA (300-fold dilution ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was generated using 500 ng of total RNA with the iSynthesis cDNA kit (Bio-Rad #1708891). RT-qPCR was performed on a CFX Connect Real-Time Detection System (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... 16uL of RNA isolated from fractions was reverse transcribed (iScript cDNA synthesis kit, Bio-Rad cat# 1708840) followed by qPCR (iTaq SYBRgreen Supermix ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein concentration of each sample was determined using the DC Protein Assay kit (BioRad, Hercules, CA). The proteins were resolved by SDS-PAGE using a Mini-Protean system (BioRad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The Luminex kits EGFR Y1068-p and p-AKT is S473-p were obtained from Bio-Rad. The capture AXL antibody was generated by conjugating a primary AXL antibody (R&D systems MAB154 ...
-
bioRxiv - Bioengineering 2023Quote: ... protein transfer was performed viaTrans-Blot® Turbo™ RTA Mini PVDF Transfer Kit (Bio-Rad, USA). The membrane was blocked with PBS-T (0.1% Tween-20 ...
-
bioRxiv - Cell Biology 2023Quote: ... After the protein concentration was measured via Lowry assay using DCTM Protein Assay Kit (BIO-RAD, 5000111), the same amount of total protein was used for each pulldown (2-3 mg per pulldown) ...
-
bioRxiv - Neuroscience 2023Quote: ... Total brain cDNA was generated from 1.25µg of total RNA using the iScript cDNA synthesis kit (BIORAD).
-
bioRxiv - Systems Biology 2023Quote: ... the sequencing library was prepared with SEQuoia Express Stranded RNA Library Prep Kit (Bio-Rad, CA, USA). The ribosome profiling and RNA-Seq libraries were sequenced on a HiSeq X Ten platform (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: ... Messenger RNA was converted to the first-strand cDNA using iScript cDNA synthesis kit (Bio-Rad #1708891), followed by RT-PCR reaction using PowerUp SYBR Green PCR Master Kit (Applied Biosystems #A25742 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mRNA was reverse transcribed using iScriptTM gDNA Clear cDNA Synthesis Kit (cat# 1725035, Bio-Rad Laboratories Ltd).
-
bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
-
bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from tissues using AurumTM Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad). Contaminating DNA was removed by treating the samples with DNase I (Bio-Rad) ...
-
bioRxiv - Immunology 2024Quote: ... and reverse transcribed to cDNA using oligo-DT primers and the iScript cDNA Synthesis Kit (Bio-Rad). Immunoglobulin heavy and light chain cDNA was amplified using the Q5 High-Fidelity 2X Master mix (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... RT-PCR was then performed to obtain corresponding cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad). qPCR was performed by CFX ConnectTM system (Bio-Rad ...
-
bioRxiv - Physiology 2024Quote: ... Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II, Cat# 5000112, Biorad). Proteins were diluted in 4x Laemmli sample buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... complementary DNA (cDNA) was synthesized from the RNA using the iScript cDNA Synthesis kit (BioRad, #170-8891). Quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Genetics 2024Quote: ... 400 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad, 1708891). The cDNA region containing phased heterozygous SNP of ANXA11 – rs2573353 in WTC11141 – was amplified using the following primers ...
-
bioRxiv - Cell Biology 2024Quote: SDS-PAGE analysis was carried out either with 10% gels (TGX FastCast acrylamide Kit 10%; Bio-Rad) or precast 4-15% gradient gels (Mini-PROTEAN TGX precast gel ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were developed using the ECL kit and scanned with ChemiDoc MP Imaging system (Bio-Rad). The blots were quantified with Image Studio Lite software and GraphPad Prism were used for statistical analysis (unpaired t test ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... total protein levels were determined using a DC protein assay kit (Bio-Rad Laboratories GmbH, Munich, Germany), and equal protein concentrations were adjusted ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR was performed using the PerfectStart Green qPCR SuperMix kit (TransGen Biotech ...
-
bioRxiv - Cancer Biology 2024Quote: ... One μg RNA was converted to cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA) and 1:10 dilution of cDNA was used for each qPCR reaction ...
-
bioRxiv - Molecular Biology 2024Quote: Protein estimation was performed in cell lysates by BCA protein assay kit (Bio-Rad, Hercules, CA, USA). For Western blots ...
-
bioRxiv - Cancer Biology 2024Quote: ... First strand cDNA was generated from isolated RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708890) and RT-qPCR performed on the resulting cDNA (diluted 5-fold ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1 µg RNA samples using the iScript cDNA Synthesis kit (Bio-Rad, USA). qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). The cDNA was diluted 1:5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined according to the Bio-Rad Protein Assay Kit II (Bio-Rad cat. 5000006). Once quantified ...
-
bioRxiv - Cell Biology 2024Quote: ... This was followed by reverse transcription and cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad). The cDNA was then used for qPCR analysis with iTaq™ Universal SYBR®Green Supermix (Bio-rad) ...
-
bioRxiv - Physiology 2024Quote: ... Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II, Cat# 5000112, Biorad). Proteins were diluted in 4x Laemmli sample buffer ...
-
bioRxiv - Genomics 2024Quote: Extracted viral (and control) RNAs were reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad, 1708891), per manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... then reverse-transcribed using iScriptTM cDNA synthesis kit (cat. nr. 1708891) (Bio-Rad Laboratories, Hercules, CA, USA) following the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was carried out using iTaq Universal SYBR Green One-Step Kit (Bio-Rad, #1725150). 50 ng of RNA was mixed with primers for targeted genes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used for reverse transcription using an iScript cDNA synthesis kit (Bio-Rad). cDNA was diluted 5 times in Milli-Q water and used as a template for PCR amplification using GoTaq G2 Green Master Mix (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
-
bioRxiv - Bioengineering 2024Quote: ... Reverse transcription into cDNA was performed according to iScript manufacturer instructions (iScript cDNA synthesis kit, Biorad 1708891). Gene expression was measured by Taqman qPCR on the cDNA using 20 μl reactions ...
-
bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred onto nitrocellulose membranes using Trans-Blot® TurboTM Transfer System RTA Transfer Kit (BioRad). Blotted membranes were blocked in 10% non-fat milk in PBS-Tween 20 0.05% for 30 minutes at room temperature ...