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Citations for Bio-Rad :
2951 - 3000 of 3153 citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were conducted using 1 μl of cDNA product in 10 μl total reaction volume using Itaq Universal SYBR green (Bio-Rad, #1725121) with primers at a concentration of 500nM ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... a total of 20 µL of 1 µg/µL plasmid DNA was combined with 50 µL of gold microparticles (1 µm, 50 mg/ml, suspended in 50% (v/v) sterile glycerol) (#1652263, Bio-Rad, USA), 100 µL 2.5 M CaCl2 and 40 µL 0.1 M spermidine (S0266 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark; Bio-Rad Laboratories) at 490 nm.
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... concentrated fractions containing CDT holotoxin were loaded onto a gravity flow size exclusion column (1 g, P-60 resin; #1504164, Bio-Rad, USA) pre-equilibrated with PBS pH 7.4 as the mobile phase and 500 μL elution fractions taken.
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bioRxiv - Molecular Biology 2024Quote: ... 200 μL aliquots were mixed with 1 μg of the plasmid and transferred to 0.2 cm electro cuvettes (BIO-RAD, Hercules, USA), chilled for 15 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were prepared at a concentration of 1 μg/μL and 15 μL of sample was loaded onto 4-15% gradient SDS-polyacrylamide gels (Bio-Rad Laboratories). Following the electrotransfer of proteins onto PVDF membranes ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µl of cDNA was used as a template for qRT-PCR using iQTM SYBR® Green Supermix (BIO-RAD, USA) on a CFX96TM Real-Time PCR machine (BIO-RAD ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.5 μL of forward and reverse primers (see Supplementary Table 1 for primer sequences) for the genes of interest were combined in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a CFX384 Real Time PCR Detection System using the following program ...
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bioRxiv - Developmental Biology 2024Quote: ... see Supplementary Table 1 for primer sequences) and diluted to 20 μL with nuclease free water in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a Bio-Rad CFX384 Real Time PCR Detection System with the following program ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with fresh serum-free medium containing 10% CCK-8 for 1 hour at 37°C and then the absorbances at 450 nm were measured using a microplate reader (Bio-Rad, Hercules, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg DNA from each sample was transferred to a nitrocellulose membrane using the Bio-Dot SF microfiltration apparatus (Bio-Rad, #170-6542). The membrane was blocked in 5% non-fat milk in TBS with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.08 of each M13 dye and 1 μL of the DNA template using a Bio-Rad thermocycler T100 (Bio-Rad, Pleasanton, CA, USA). PCR products were analyzed on an ABI 3500 genetic analyzer and sized against the LIZ500 internal standard (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
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bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Biophysics 2019Quote: ... The relative amount of present HRP was detected by adding 60 µL/well DPBS + 1 mM CaCl2 and 20 µl/well HRP substrate (Bio-Rad, 170-5060). The plate was incubated for 10 minutes at room temperature before recording of luminescence on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and then subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent loading of total protein ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were synthesized from 1 μg total RNAs and qPCR were performed by using the iScript™ cDNA Synthesis Kit (BioRad, the Netherlands) and iQ™ SYBR Green Supermix (BioRad ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Immunology 2021Quote: ... Transcript expression was measured by qRT-PCR with the appropriate primers (Supplemental Table 1) using a Bio-Rad MyiQ™ Real-Time PCR Detection System (Bio-Rad Laboratories) to measure SYBR Green (IQ™ SYBR® Green Supermix ...
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bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... cys free or AAA SNAREs were reconstituted at a 1:400 protein:lipid ratio of the same lipid compositions by dialysis in the presence of Bio-Beads (Bio-Rad Laboratories, Hercules, CA) into metal-free buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
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bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription (RT) was performed with 1 μg of total RNA using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 172-5272). cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... After three washes (5, 10, 15 minutes) the blot was incubated for 60 minutes with secondary Goat Anti-Rabbit-HRP (Bio-Rad, 1:7000) and Alexa 647 Goat-Anti-Mouse antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% skimmed milk in TBST for 30 min at room temperature then incubated with primary antibodies overnight at 4°C and secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at room temperature before visualization by ECL Plus Western blotting detection reagent (Bio-Rad, Hercules, CA). Semi-quantification of the bands were performed using NIH Image J software.
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
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bioRxiv - Immunology 2022Quote: ... The affinity matrix was resuspended in 150 μl PB for the unbound and full plasma fraction and in 40 μl PB with a 1% blocking buffer background (Bio-Rad, The Netherlands) for the bound fractions ...