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Citations for Bio-Rad :
2951 - 3000 of 9890 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Neuroscience 2022Quote: Protein sample was loaded into each well of a 4-20% Mini-PROTEAN TGX Precast protein 15-well gels (Bio-Rad, cat#4561096). Protein was transferred using transfer buffer (20% MeOH in TBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed using RIPA buffer and total protein concentration was quantified using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006). Twenty micrograms of protein were incubated in Laemmli sample buffer at 95°C for 5 min and subjected to electrophoresis on a 3-15% gradient polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A total of 20 μg of protein was separated using a 4%–15% Criterion™ XT tris-glycine protein gel (BioRad, United States) and then transferred to nitrocellulose membrane (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration in samples was determined with the Bradford assay using a Protein Assay Dye Reagent (#500-0006, Bio-Rad, Richmond, CA) with optical density reading at 595 nm (OD595) ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria-containing pellet was resuspended in THE buffer without BSA and the protein concentration measured using the Bradford Protein Assay (Bio-Rad, Munich, Germany). The protein concentration was adjusted to 10 µg/µl with THE buffer.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... and protein-bound beads were washed with 60ml lysis buffer until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Biochemistry 2023Quote: Final carboxysome samples and as well as the lysate were analyzed for presence of CsoSCA or sfGFP-fusion protein by SDS-page (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad)) ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a new microcentrifuge tube and protein concentration was determined by using a DC protein assay kit (BioRad, Cat# 500-0116). Cell lysates (500-1000 μg ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μg of each protein sample was separated by electrophoresis and proteins were then transferred onto a Trans-Blot® Turbo™ PVDF Membrane (Biorad 10026933) at 2.5 Å for 3 minutes using Trans-Blot® Turbo™ Transfer System (Biorad 1704150) ...
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bioRxiv - Plant Biology 2023Quote: ... Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad, Hercules, USA) and separated at 4°C at 150 constant volts ...
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bioRxiv - Immunology 2023Quote: ... and equal protein amounts were separated using ready-to-use 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Biorad, 4568096) and transferred onto 0.2 μm pore nitrocellulose membranes (PALL Life Sciences 66485).
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bioRxiv - Genomics 2023Quote: ... 20-25ug of protein lysate was loaded per well and separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (Bio-Rad). Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... protein content in the soluble fraction of homogenates was measured using a RC-PC protein assay kit (DC Protein Assay Kit, Bio-Rad, Hercules, CA), and then dissolved in Laemmli buffer.
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bioRxiv - Bioengineering 2024Quote: ... and 8ug of total protein from each lysate was separated on a 4-15% SDS-polyacrylamide gel (Mini-PROTEAN® TGX™ Precast Protein Gels (BIO-RAD). After the proteins were transferred to nitrocellulose membrane (BIO-RAD) ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of protein per sample were loaded into each lane of 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were transferred to nitrocellulose membrane using semidry Trans- Blot® Turbo™ Transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were cleared by centrifugation at 10,000 g for 5 minutes and protein concentration was determined using the DC Protein Assay (Bio-Rad Laboratories, Watford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 ug of protein was loaded in each lane of a 4– 15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad 4561083). After SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein cleavage was carried out at room temperature and was monitored by SDS-PAGE (Bio-Rad Mini-Protein Precast TGX Gels 12%). The final protein aggregates were collected after 4 days ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was transferred to a clean tube and total protein concentration was measured with the DC protein assay II (Bio-Rad #500-0112). Samples were stored at -80 until used.
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bioRxiv - Neuroscience 2024Quote: ... and a total of 50 µg protein was loaded on the Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) or 4–12% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) ...
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bioRxiv - Systems Biology 2024Quote: ... From each sample 20 µg of total protein was loaded and separated on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad), followed by electroblotting onto membranes of Trans-Blot Turbo Midi 0.2 µm PVDF Transfer Packs (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 30μg of total protein lysate of each sample was separated by electrophoresis in 12% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and transferred using a Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: 20 μg of protein extracts were loaded and separated using 12% acrylamide gels (Criterion™ TGX Stain-Free™ Protein Gel (Biorad, 5678044) and blotted on PVDF membranes (AmershamTM Hybond ...
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bioRxiv - Cell Biology 2024Quote: ... 30ug of protein as determined by Bradford protein quantification assay was loaded onto a mini-PROTEAN TGX stain-free gel (Bio-Rad, cat#4568084) and run at 200V for 25 minutes in SDS running buffer (.2501M Tris base (Fisher ...
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bioRxiv - Cancer Biology 2024Quote: Protein concentrations were determined by the Bio-Rad Protein Assay Dye Reagent Concentrate protein assay according to the manufacturer’s instructions (Bio-Rad catalog No. 5000006). Protein samples were separated by standard SDS-PAGE method and transferred to PVDF membranes ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were run on a 10% SDS-PAGE gel with the Precision Plus Protein All Blue Prestained Protein Standard (BioRad Laboratories, Hercules, CA) and transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Bradford Protein assay method was used to determine the protein concentration using bovine serum albumin (BSA) as the standard (Bio-Rad catalog 500-0006). 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 μg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... Sample proteins were extracted using RIPA buffer and the concentration protein in the supernatant was determined by the Bradford method (Bio-Rad Lab, California, USA). Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Genetics 2020Quote: ... the soluble fraction was transferred to a new tube and total protein concentration was determined by DC protein assay (Bio-Rad, Hercules, CA, USA). Following quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... The fluorescence intensity was calculated relative to protein concentration in each sample as assessed by the DC™ Protein Assay (BioRad, Hercules, CA, USA) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... separated by electrophoresis and transferred to nitrocellulose membrane accordingly.1 The molecular mass of protein was determined by comparison with the migration pattern of Precision Plus Protein™ Prestained Standards Dual Color (Biorad Laboratories, CA, USA). The primary antibodies used were anti-pAKT (S473) ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein levels were normalized to the unphosphorylated version of the protein or the total protein content per lane as assessed via stain-free TGX gel technology (Bio-Rad, Hercules, CA, USA) [49] ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).