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Citations for Bio-Rad :
251 - 300 of 3749 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A total of 50 ug of WCL was combined with 4X Laemelli Sample Buffer (BioRad, 1610747) to a final concentration of 1X sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 20 ug protein was analyzed by 4%–15% gradient TGX Precast polyacrylamide gel (Bio-Rad), transferred onto a PVDF membrane (Biorad Transblot Turbo system ...
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bioRxiv - Cancer Biology 2019Quote: ... and F4/80 (Cl:A3-1, Bio-Rad, 1:1000) staining were performed by the Duke Pathology core (Durham ...
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bioRxiv - Systems Biology 2023Quote: ... F4/80 (Bio-Rad, clone: Cl:A3-1, 1:80), TIM4 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... anti-WC-1 (Bio-Rad, MCA838GA, dilution 1:20) and anti-Pax5 (Agilent DAKO ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Plant Biology 2021Quote: ... the Agrobacterium-infiltrated leaves were visually inspected at 2-5 days post infiltration and/or the fluorescence of the infiltrated leaves were monitored at 1-2 d post infiltration under the Pro-Q Emerald 488 in the ChemiDoc™ Gel Imaging System (Bio-Rad, Hercules, CA, USA) as described previously (Yoon and Rikkerink 2020).
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: A 1 mL Ni-NTA resin in a glass gravity-flow column (Econo-Column®, BioRad, USA) was set up inside a Coy anaerobic glovebox with an atmosphere of 100% N2 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant contained 2.5 mg proteins/ 1 ml (determined using Bio-Rad Protein Assay, Cat. No. 5000006). This protein fraction was used for co-immunoprecipitation (input) ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 was concentrated to 1-mL and isocratically eluted from an ENrich SEC650 10×300 column (BioRad) equilibrated with Buffer B ...
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Cancer Biology 2023Quote: 6-well culture plates were coated with 1 ml of 0.6 % Low Melt Agarose (Bio-Rad; 1613111). Cells were diluted at a density of 1.0 x 104 cells/ml in media containing 0.3% Low Melt Agarose and seeded 1 ml/ well ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... One ml of sterile Bio-Gel P-100 polyacrylamide beads (Bio-Rad, 150-4174) in PBS (2% w/v ...
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bioRxiv - Cell Biology 2023Quote: ... loaded with a 100 mg/ml Biogel P-10 Gel (Bio-Rad, Hercules, CA) solution ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
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bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
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bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Cell Biology 2021Quote: ... 15 ug of protein were separated by 4-20% Mini Protean TGX SDS-polyacrylamide gels (Bio-Rad) at 120 V for 1 h and transferred onto 0.2 um PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... 10 ug of total protein was loaded per lane onto a 12% SDS-PAGE gel (Bio-Rad), which was run at 100 V for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... 10-30 ug total protein were resolved by SDS-PAGE and transferred to PVDF membranes (Bio-Rad). Primary antibody incubations were done overnight with antibodies diluted in 3% BSA in TBS-T (Tris-buffered saline/Tween 20) ...
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bioRxiv - Bioengineering 2024Quote: Purified antibodies (0.5 ug each) were denatured by adding 4X LaemmLi sample buffer (Bio-Rad, cat.: 1610747) and 2-mercaptoethanol and heating at 100°C for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Tween 20) at room temperature for 1 hour and stained with 0.3 μg/mL streptavidin-HRP (BioRad) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... HRP detection was performed by adding 1 mL Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Genetics 2023Quote: ... 1 ml 2xYNB with 2% glucose was added to the sorted cells in sheath fluid (PBS, BioRad #12012932) and they were transferred to a shaking incubator and grown at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 1 ml HisTrap (Cytiva) column connected to NGC Quest 10 (Bio-Rad), previously equilibrated with Buffer A (25 mM Tris–HCl pH 8.0 ...