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Citations for Bio-Rad :
251 - 300 of 8422 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Microbiology 2019Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (BioRad), 1 μl of 20× WT primers/probe ...
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Comparative regulomics reveals pervasive selection on gene dosage following whole genome duplicationbioRxiv - Evolutionary Biology 2020Quote: ... Nuclei were counted on an automated cell counter (TC20 BioRad, range 4-6 um) and further confirmed intact under microscope ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counted on an automated cell counter (TC20 BioRad, range 4-6 um) and further confirmed intact under microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons at DIV 4-6 were transfected using a biolistic gene gun (Bio-Rad) and were assayed 3 days after transfection as described previously (5,6,26,27) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
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bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
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bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... We ran the qPCR reactions using 1-2 µl cDNA in SYBR Green Master Mix (Biorad) totaling 20 µl in a Biorad CFX96 machine using 60°C as the annealing temperature ...
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bioRxiv - Biophysics 2020Quote: ... Nanodisc reconstitution was achieved by incubation with 0.5 - 1 mL Bio-Beads SM-2 (Bio-Rad) for 16 hours at 4°C under constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in monomer solution (1 x PBS, 2 M NaCl, 2.5% acrylamide (Bio-Rad), 0.15% Methylenbisacrylamide (Santa Cruz) ...
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bioRxiv - Genetics 2022Quote: ... 1-2 μg RNA was used for cDNA synthesis using the iScript Advanced kit (Bio-Rad), following the manual.
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bioRxiv - Microbiology 2022Quote: ... Commercial antibodies were used for β-tubulin (clone YL1/2, Bio-Rad, Watford, UK; 1:5000), β-actin (Abcam ab8227 ...
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bioRxiv - Microbiology 2021Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (Bio-Rad), 1 μl of 20× WT primers/probe ...
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bioRxiv - Microbiology 2019Quote: ... Each PCR was performed in a total volume of 10 µL containing 5 µL of 2 × iQSYBR Green supermix (Bio-Rad, USA), 200 nM of each primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated for 1-2 h at RT with an anti-rabbit HRP-conjugated secondary antibody (diluted 1 : 3,000, Bio-Rad) in TBST with 5% milk ...
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bioRxiv - Cancer Biology 2021Quote: ... to bis-acrylamide (2% w/v; Bio-Rad) was varied to tune gel stiffness to 20 kPa ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). The Q-PCR primer sequences are in Additional file 8 ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). The Q-PCR primer sequences are included in Additional file 8.
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bioRxiv - Biochemistry 2020Quote: ... SM-2 Bio-Beads was from Bio-Rad Lab. ...
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bioRxiv - Biochemistry 2020Quote: ... SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl of 2×ddPCR Supermix (Bio-Rad), wild-type and mutant allele-specific TaqMan probes at a concentration of 0.25 μM ...
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bioRxiv - Biophysics 2020Quote: ... Then activated Bio-Beads SM-2 (Bio-Rad), ∼300 uL ...
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bioRxiv - Microbiology 2022Quote: 2 mm Electroporation Cuvettes (Bio-Rad, catalog #: 1652092).
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bioRxiv - Immunology 2019Quote: ... 2% low melting agarose (CleanCut agarose, Bio-Rad) was melted at 70 °C followed by incubation at 43 °C for 10 min ...
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bioRxiv - Bioengineering 2019Quote: ... and 2%bis-acrylamide (Bio-Rad 161-0142) were mixed with ammonium persulfate (APS ...
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bioRxiv - Microbiology 2020Quote: ... and CD16-FITC (clone AT154-2, Bio-Rad) in RPMI 1640 media for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... A 2 % Agarose gel (Bio-Rad, California, USA) was prepared ...
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bioRxiv - Molecular Biology 2020Quote: ... pre-equilibrated Bio-Beads SM-2 (Bio-Rad) was added in batch and the sample was mixed end-over-end overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and the DNA Engine Opticon 2 system (BioRad). Q-PCR primer sequences as follows ...
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bioRxiv - Cell Biology 2020Quote: ... 2% v/v β-mercaptoethanol (Bio-Rad, 1610710) and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2-mercaptoethanol (Bio-Rad Laboratories B.V.) and incubated at 100°C for 6 min ...
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bioRxiv - Genomics 2021Quote: ... 2% low melting agarose (CleanCut agarose, Bio-Rad) was melted at 70 °C followed by incubation at 43°C for 10 min ...
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bioRxiv - Genomics 2021Quote: ... 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... Damp Bio-Beads SM-2 resin (Bio-Rad) was added to the mixture for 3 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and 2% bis-acrylamide solution (Bio-Rad, 1610142) and Sigma molecular biology grade water to achieve different final stiffness ...